Challenges and perspectives in porcine model of acute lung injury using oleic acid

Borges, Aline Morás; Ferrari, Renata S.; Thomaz, Leonardo Dalla Giacomassa Rocha; Ulbrich, Jane Maria; Félix, Elaine Aparecida; Silvello, Daiane; Andrade, Cristiano Feijó

doi:10.1016/j.pupt.2019.101837

Cited by 12 publications

(17 citation statements)

References 22 publications

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“…To better control the experimental conditions, we chose oleic acid to establish the ARDS model. The model has similarities regarding the basic clinical features of ARDS patients [ 21 , 24 ]. There are many methods of mechanical ventilation for ARDS, and the reasons why we choose the BIPAP ventilation mode are as follows: First, the BIPAP ventilation mode is different from the A/C-V mode in that it is an unassisted SB ventilation mode, which can occur at any stage of the mechanical ventilation cycle and is only related to the respiratory drive of the experimental animals themselves, The main feature of BIPAP is that SB can be allowed during any phase of the mechanical cycle, so it is easy to maintain comparable levels of ventilator support between BIPAP with SB and without SB.…”

Section: Discussionmentioning

confidence: 99%

“…Mechanical ventilation can lead to VILI through a series of mechanisms [ 27 ]. Some animal experiments have shown that severe SB in ARDS patients may aggravate a lung injury, and neuromuscular blockers can alleviate a lung injury [ 21 , 25 ]. However, it is worth noting that both studies used A/C-V and A/C-P ventilation modes, which are more likely to cause human–machine asynchrony and respiratory superposition, which produces gas traps.…”

Section: Discussionmentioning

confidence: 99%

“…When OI < 100 mmHg and stabilized for 30 minutes. It shows that the serious ARDS model is manufactured successfully [ 20 , 21 ]. After inducing lung injury, the lung was reopened, using BIPAP mode.…”

Section: Methodsmentioning

confidence: 99%

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Biphasic positive airway pressure spontaneous breathing attenuates lung injury in an animal model of severe acute respiratory distress syndrome

et al. 2022

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Objective To compare the effects of unassisted spontaneous breathing (SB) and complete muscle paralysis (PC) on early severe acute respiratory distress syndrome (ARDS) in an animal model, and to explore the possibility of biphasic positive airway pressure (BIPAP) as lung protective ventilation support for patients in the early stage of severe ARDS. Methods Twelve healthy beagle dogs between the ages of 10 and 15 months were randomly divided into two groups: the SB group (BIPAPSB) and the PC group (BIPAPPC). Arterial blood samples were drawn before modelling. Arterial blood gas analysis and mechanical tests were conducted. The animal model of severe ARDS was established using a deep intravenous injection of oleic acid, and BIPAP ventilation was performed for 8 hours. Lung tissue and blood were taken to detect lung function, inflammatory reactions and degree of pathological damage. Results At the beginning of the experiment, there was no significant difference in the arterial blood gas analysis between the two groups (p > 0.05). After successful modelling, the oxygenation index and the end-expiratory lung volume in the SB group were significantly higher than those in the PC group 8 hours after MV. Pathologically, the wet-dry ratio and pathological score of the PC group were higher than those of the SB group; the lung injury in the gravity-dependent area in the SB group was less than that in the PC group (p< 0.05). Conclusions In the early stage of severe ARDS induced by oleic acid, compared with PC, retention of the BIPAP mode of SB can reduce the risk of lung injury and improve respiratory function.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Biphasic positive airway pressure spontaneous breathing attenuates lung injury in an animal model of severe acute respiratory distress syndrome

et al. 2022

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“…Acute lung injury (ALI) is a life-threatening condition characterized by high incidence and mortality rates (Borges et al. 2019 ). Traumatic injuries may trigger the occurrence of ALI, and they are grossly divided into two categories: direct and indirect injury (Chimenti et al.…”

Section: Introductionmentioning

confidence: 99%

Pterostilbene pre-treatment reduces LPS-induced acute lung injury through activating NR4A1

Liu

Wang

et al. 2022

Pharmaceutical Biology

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Context Pterostilbene (PTE), a common polyphenol compound, exerts an anti-inflammatory effect in many diseases, including acute lung injury (ALI). Objective This study explores the potential mechanism of PTE pre-treatment against lipopolysaccharide (LPS)-induced ALI. Materials and methods Sixty Sprague-Dawley rats were divided into control, ALI, 10 mg/kg PTE + LPS, 20 mg/kg PTE + LPS, and 40 mg/kg PTE + LPS groups. At 24 h before LPS instillation, PTE was administered orally. At 2 h before LPS instillation, PTE was again administered orally. After 24 h of LPS treatment, the rats were euthanized. The levels of inflammatory cells and inflammatory factors in the bronchoalveolar lavage fluid (BALF), the expression of nuclear receptor subfamily 4 group A member 1 (NR4A1), and the nuclear factor (NF)-κB pathway-related protein levels were detected. NR4A1 agonist was used to further investigate the mechanism of PTE pre-treatment. Results After PTE pre-treatment, the LPS induced inflammation was controlled and the survival rate was increased to 100% from 70% after LPS treatment 24 h. For lung injury score, it decreased to 1.5 from 3.5 after treating 40 mg/kg PTE. Compared with the control group, the expression of NR4A1 in the ALI group was decreased by 20–40%. However, the 40 mg/kg PTE pre-treatment increased the NR4A1 expression by 20–40% in the lung tissue. The results obtained with pre-treatment NR4A1 agonist were similar to those obtained by pre-treatment 40 mg/kg PTE. Conclusions PTE pre-treatment might represent an appropriate therapeutic target and strategy for preventing ALI induced by LPS.

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“…El protocolo que a continuación se presenta es la síntesis de la adopción, modificación y creación de protocolos similares, descritos en la literatura científica, que permite la confiabilidad de los datos obtenidos pero que preservarán la seguridad del equipo de trabajo en el marco normativo bioético que regulan la investigación en animales (16)(17)(18)(19)(20) . Además, representa el esfuerzo de la academia, la empresa y el estado por ofrecer una solución a la región y al país, en medio de una crisis de salud mundial que debe hacer surgir lo mejor de las instituciones y su intento de preservar la salud y la vida.…”

Section: Introductionunclassified

Protocolo de prueba de ventilador mecánico para atención de pacientes con COVID-19 en modelo porcino

García-Castro

Latorre-Galeano

Giraldo-Montoya

et al. 2021

Rev Peru Med Exp Salud Publica

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A raíz de la pandemia generada por el SARS-CoV-2, los sistemas de salud de los distintos países han experimentado un dramático aumento en la ocupación de las unidades de cuidado intensivo y, por ende, una alta demanda de ventiladores mecánicos. En este artículo se propone un protocolo de pruebas de ventiladores mecánicos de bajo costo en modelos porcinos, como parte del desarrollo de equipos biomédicos que pueden apoyar la crisis sanitaria suscitada por la pandemia. El protocolo aborda aspectos que incluyen la fase previa a la prueba, el manejo anestésico, de vía aérea, seguimiento de laboratorio, recuperación y seguimiento del animal. Como resultado, se presenta a la comunidad académica un protocolo de validación de prototipo de ventilador mecánico en espécimen porcino como un referente para su aplicación y revalidación por parte de grupos interesados en el desarrollo de tecnologías locales de bajo costo.

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Challenges and perspectives in porcine model of acute lung injury using oleic acid

Cited by 12 publications

References 22 publications

Biphasic positive airway pressure spontaneous breathing attenuates lung injury in an animal model of severe acute respiratory distress syndrome

Biphasic positive airway pressure spontaneous breathing attenuates lung injury in an animal model of severe acute respiratory distress syndrome

Pterostilbene pre-treatment reduces LPS-induced acute lung injury through activating NR4A1

Protocolo de prueba de ventilador mecánico para atención de pacientes con COVID-19 en modelo porcino

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