Challenges and opportunity of recent genome editing and multi-omics in cyanobacteria and microalgae for biorefinery

Lin, Way-Rong; Tan, Shih‐I; Hsiang, Chuan-Chieh; Sung, Po-Kuei; Ng, I‐Son

doi:10.1016/j.biortech.2019.121932

Cited by 80 publications

(35 citation statements)

References 148 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…From a biorefinery point of view, improving TAG content and yield has great potential to bring down the production cost of algal biodiesel. Besides the screening of algal strains and manipulation of culture conditions, genetic engineering represents a feasible strategy to break the inherent limit of algae for trait improvements including TAG content and/or yield (Lin et al, 2019 Enhancing TAG production by algae via genetic engineering can be facilitated by the understanding of pathways and regulatory mechanisms for lipid metabolism (Goncalves et al, 2016;Lenka et al, 2016;Li-Beisson et al, 2019). It is believed in algae that a series of coordinated biological pathways that provide carbon precursors, energy and reductant molecules, pushing and pulling power, and storage sink are stimulated to contribute to TAG synthesis and accumulation (Li et al, 2014;Liu et al, 2019;Park et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Lipid production is more than doubled by manipulating a diacylglycerol acyltransferase in algae

Pan

Ding

et al. 2020

GCB Bioenergy

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Lipid production is more than doubled by manipulating a diacylglycerol acyltransferase in algae

Pan

Ding

et al. 2020

GCB Bioenergy

View full text Add to dashboard Cite

show abstract

“…In recent years, due to the unique characteristics such as efficient photosynthesis, rapid growth, simple structure, and convenient genetic manipulations, cyanobacteria serve as promising microbial platforms for artificially designing, constructing, and controlling photosynthesis-driven routes for directional conversion of energy and materials ( Lu, 2010 ; Desai and Atsumi, 2013 ). Based on the development and utilization of system biology technologies (multiple -omics approaches), massive information about the metabolic profiles and dynamics of cyanobacteria cells under stable and changing environments have been acquired ( Aikawa et al, 2019 ; Lin et al, 2019 ). Notable improvements in developing efficient tools of synthetic biology and metabolic engineering over the last decade have permitted effective regulation and expansion of the photosynthetic metabolism network ( Sun et al, 2018b ; Santos-Merino et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Systematic Identification of Target Genes for Cellular Morphology Engineering in Synechococcus elongatus PCC7942

et al. 2020

View full text Add to dashboard Cite

Cyanobacteria are serving as promising microbial platforms for development of photosynthetic cell factories. For enhancing the economic competitiveness of the photosynthetic biomanufacturing technology, comprehensive improvements on industrial properties of the cyanobacteria chassis cells and engineered strains are required. Cellular morphology engineering is an up-and-coming strategy for development of microbial cell factories fitting the requirements of industrial application. In this work, we performed systematic evaluation of potential genes for cyanobacterial cellular morphology engineering. Twelve candidate genes participating in cell morphogenesis of an important model cyanobacteria strain, Synechococcus elongatus PCC7942, were knocked out/down and overexpressed, respectively, and the influences on cell sizes and cell shapes were imaged and calculated. Targeting the selected genes with potentials for cellular morphology engineering, the controllable cell lengthening machinery was also explored based on the application of sRNA approaches. The findings in this work not only provided many new targets for cellular morphology engineering in cyanobacteria, but also helped to further understand the cell division process and cell elongation process of Synechococcus elongatus PCC7942.

show abstract

“…Researchers have discovered biological information of a few microalgae species such as Chlamydomonas reinhardtii , Chlorella variabilis , and Chlorella sorokiniana 23‐25 . These species are still in the stage of genetic modification 26 …”

Section: Introductionmentioning

confidence: 99%

Sustainability of the four generations of biofuels – A review

et al. 2020

View full text Add to dashboard Cite

Biofuel has emerged as an alternative source of energy to reduce the emissions of greenhouse gases in the atmosphere and combat global warming. Biofuels are classified into first, second, third and fourth generations. Each of the biofuel generations aims to meet the global energy demand while minimizing environmental impacts. Sustainability is defined as meeting the needs of the current generations without jeopardizing the needs of future generations. The aim of sustainability is to ensure continuous growth of the economy while protecting the environment and societal needs. Thus, this paper aims to evaluate the sustainability of these four generations of biofuels. The objectives are to compare the production of biofuel, the net greenhouse gases emissions, and energy efficiency. This study is important in providing information for the policymakers and researchers in the decision-making for the future development of green energy. Each of the biofuel generations shows different benefits and drawbacks. From this study, we conclude that the first generation biofuel has the highest biofuel production and energy efficiency, but is less effective in meeting the goal of reducing the greenhouse gases emission. The third generation biofuel shows the lowest net greenhouse gases emissions, allowing the reduction of greenhouse gases in the atmosphere. However, the energy required for the processing of the third generation biofuel is higher and, this makes it less environmentally friendly as fossil fuels are used to generate electricity. The third and fourth generation feedstocks are the potential sustainable source for the future production of biofuel. However, more studies need to be done to find an alternative low cost for biofuel production while increasing energy efficiency.

show abstract

Challenges and opportunity of recent genome editing and multi-omics in cyanobacteria and microalgae for biorefinery

Cited by 80 publications

References 148 publications

Lipid production is more than doubled by manipulating a diacylglycerol acyltransferase in algae

Lipid production is more than doubled by manipulating a diacylglycerol acyltransferase in algae

Systematic Identification of Target Genes for Cellular Morphology Engineering in Synechococcus elongatus PCC7942

Sustainability of the four generations of biofuels – A review

Contact Info

Product

Resources

About