Challenges &amp; Solutions for Recent Advancements in Multi-Drugs Resistance Tuberculosis: A Review

Yadav, Pramod

doi:10.1177/11786361231152438

Cited by 12 publications

(6 citation statements)

References 46 publications

(68 reference statements)

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“…Within the phagolysosome, a potent arsenal of reactive oxygen species (ROS) unleashes oxidative mayhem upon invaders. 25 This initial skirmish triggers the recruitment of mononuclear leukocytes, bolstering the immune response. These professional phagocytes constitute the first line of defense, actively engaging and eliminating mycobacterial threats.…”

Section: Macrophages In Tuberculosis (Tb)mentioning

confidence: 99%

From Bronchial Asthma to Tuberculosis: Unravelling the Cytokine-Macrophage Axis in Diverse Diseases

Yadav

2024

SOJMCCR

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Cytokines, tiny proteins secreted by cells, play a critical role in mediating communication and interactions between cells. They also function as immunomodulating agents, adjusting immune responses. When released into the bloodstream or tissues, cytokines bind to specific receptors on target immune cells, triggering cellular responses. Cytokines are implicated in various diseases, including asthma, COPD, HIV infection, and multiple sclerosis. This review delves into the intricate interplay between cytokines and macrophages, focusing on their roles in inflammation and the immune response. Macrophages, which are scavenger cells of the immune system, exhibit remarkable heterogeneity, reside in diverse tissues, and play crucial roles in both innate and acquired immunity. They can be activated for proinflammatory or anti-inflammatory functions, contributing to tissue destruction or regeneration. Cytokines influence macrophage activation and polarization, impacting the inflammatory process. Dysregulated cytokine production and macrophage activation are observed in various diseases. For example, in asthma, an imbalance in macrophage phenotypes contributes to airway hyperresponsiveness. Understanding the complex interplay between cytokines and macrophages is crucial for developing novel therapeutic strategies for inflammatory diseases. Future research directions include utilizing humanized animal models, single-cell sequencing, and immunomodulatory therapies to further decipher the intricate roles of cytokines and macrophages in health and disease. Ultimately, elucidating these interactions holds immense potential for improving human health outcomes.

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Section: Macrophages In Tuberculosis (Tb)mentioning

confidence: 99%

From Bronchial Asthma to Tuberculosis: Unravelling the Cytokine-Macrophage Axis in Diverse Diseases

Yadav

2024

SOJMCCR

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“…The fractions were collected and reapplied to a Sephadex G-200 column equilibrated with Tris-Cl buffer (50 mM and pH 7.4) and NaCl (100 mM) at a 45 ml/h flow rate. The eluted proteins were precipitated with ammonium sulfate, dissolved in a buffer with protease inhibitors, and used for SDS-PAGE and 2D-PAGE [13][14][15][16]. For the first dimension in 2D-PAGE, the protein concentration was determined by the Bradford method.…”

Section: Synovial Fluid Protein Analysismentioning

confidence: 99%

Synovial Fluid Proteomics and Serum Metabolomics Reveal Molecular and Metabolic Changes in Osteoarthritis

Yadav

2024

CRCT

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Background: Osteoarthritis (OA) is a common joint disorder with a complex and multifactorial pathogenesis. Proteomics analysis using two-dimensional gel electrophoresis (2DE) and mass spectrometry (MS) enables high-throughput identification of differentially expressed proteins related to OA. However, the etiology, pathophysiology, and early diagnostic markers of OA are still poorly understood. Methods: Synovial fluid protein biomarkers were compared between OA patients and healthy controls. It was fractionated using DEAE cellulose and Sephadex G-200 columns, followed by SDS‒PAGE and 2D-PAGE for visualization and identification. Mass spectrometry and Mascot were used for protein analysis, and serum metabolite profiles were also investigated using 1D 1H CPMG NMR spectra. Multivariate data analysis, including PCA and PLS-DA, was performed to detect metabolic differences between groups. Results: Proteomics analysis revealed differential expression of synovial fluid proteins, such as serine protease inhibitors, complement components, and apolipoproteins, which may be involved in inflammation and cartilage breakdown. Additionally, serum metabolite profiles differed significantly between OA patients and controls, involving amino acid, lipid, glucose, and energy metabolism. The pathway analysis indicated disruption of the metabolic pathways associated with these metabolites. Conclusions: This study provides insights into the molecular and metabolic changes in OA. Protein biomarkers and serum metabolite alterations enhance the understanding of OA pathogenesis and offer potential opportunities for early diagnosis and disease management. Further validation and translation of these findings into clinical applications are needed for improved OA detection and intervention strategies.

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Section: Synovial Fluid Protein Analysismentioning

confidence: 99%

Synovial fluid proteomics and serum metabolomics reveal molecular and metabolic changes in osteoarthritis

Yadav

2023

Open J Clin Med Images

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Challenges & Solutions for Recent Advancements in Multi-Drugs Resistance Tuberculosis: A Review

Cited by 12 publications

References 46 publications

From Bronchial Asthma to Tuberculosis: Unravelling the Cytokine-Macrophage Axis in Diverse Diseases

From Bronchial Asthma to Tuberculosis: Unravelling the Cytokine-Macrophage Axis in Diverse Diseases

Synovial Fluid Proteomics and Serum Metabolomics Reveal Molecular and Metabolic Changes in Osteoarthritis

Synovial fluid proteomics and serum metabolomics reveal molecular and metabolic changes in osteoarthritis

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