Chain-length dependent interfacial immunoreaction kinetics on self-assembled monolayers revealed by surface-enhanced infrared absorption spectroscopy

Bao, Wenjing; Li, Jian; Cao, Tianyuan; Li, Jin; Xia, Xing‐Hua

doi:10.1016/j.talanta.2017.08.017

Cited by 10 publications

(7 citation statements)

References 34 publications

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“…The association rate constant and balance time of antigen‐antibody interaction on the SAMs were calculated for SAMs of different chain lengths. For longer chain lengths ( n = 16), the association rate constant was 2.99 ± 0.36 × 10 6 m −1 s −1 , and the balance time was 15 min …”

Section: Biomedical Applications Of Functional Nanomaterials On 2d Sumentioning

confidence: 99%

Functional Nanomaterials on 2D Surfaces and in 3D Nanocomposite Hydrogels for Biomedical Applications

Tutar

Motealleh

Khademhosseini

et al. 2019

Adv Funct Materials

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An emerging approach to improve the physicobiochemical properties and the multifunctionality of biomaterials is to incorporate functional nanomaterials (NMs) onto 2D surfaces and into 3D hydrogel networks. This approach is starting to generate promising advanced functional materials such as self‐assembled monolayers (SAMs) and nanocomposite (NC) hydrogels of NMs with remarkable properties and tailored functionalities that are beneficial for a variety of biomedical applications, including tissue engineering, drug delivery, and developing biosensors. A wide range of NMs, such as carbon‐, metal‐, and silica‐based NMs, can be integrated into 2D and 3D biomaterial formulations due to their unique characteristics, such as magnetic properties, electrical properties, stimuli responsiveness, hydrophobicity/hydrophilicity, and chemical composition. The highly ordered nano‐ or microscale assemblies of NMs on surfaces alter the original properties of the NMs and add enhanced and/or synergetic and novel features to the final SAMs of the NM constructs. Furthermore, the incorporation of NMs into polymeric hydrogel networks reinforces the (soft) polymer matrix such that the formed NC hydrogels show extraordinary mechanical properties with superior biological properties.

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Section: Biomedical Applications Of Functional Nanomaterials On 2d Sumentioning

confidence: 99%

Functional Nanomaterials on 2D Surfaces and in 3D Nanocomposite Hydrogels for Biomedical Applications

Tutar

Motealleh

Khademhosseini

et al. 2019

Adv Funct Materials

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“…Attenuated total reflection–infrared absorption spectroscopy (ATR-IRAS) has been considered as a powerful tool for molecule-level study of interfacial structures and processes in aqueous solutions owing to the confined penetration electric field which significantly suppresses the interference from bulk water. − Recent studies have revealed that the analytical sensitivity of this technique can be significantly improved by introducing surface enhanced substrates with localized surface plasmon resonance wavenumber located in the mid-IR region, named as attenuated total reflection–surface enhanced infrared absorption spectroscopy (ATR-SEIRAS). This ATR-SEIRAS has been successfully used to study the interfacial behaviors, − the interfacial biomolecular recognition events, − the relationship between conformation and function of redox proteins under potential control, − and the influence of membrane potential on membrane proteins in situ and real time. , Although great progress has been achieved in this research area, , this new technique yet suffers from interference from the abundant existence of solvent water at the surface since water molecules are of the tremendous IR absorption cross-section . Thus, quantitative analysis in sub-nanomolar level or even lower levels of the targets at the IR enhanced interfaces with limited enhancement factor cannot be easily achieved only based on the IR features of the targets in aqueous solutions.…”

mentioning

confidence: 99%

Water as a Universal Infrared Probe for Bioanalysis in Aqueous Solution by Attenuated Total Reflection–Surface Enhanced Infrared Absorption Spectroscopy

et al. 2018

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Monitoring the properties and reactions of biomolecules at their interface has attracted ever-growing interest. Here, we propose an approach of infrared analysis technique that utilizes water molecule as a universal probe for in situ and label free monitoring of interfacial bioevents in aqueous solution with high sensitivity. The strong infrared (IR) signal of O−H stretching vibrations from the repelled water is used to sensitively reveal the kinetics of interfacial bioevents at molecular level based on the steric displacement of water using an attenuated total reflection−surface enhanced infrared absorption spectroscopy. Using interfacial immuno-recognition and DNA hybridization as demonstrations, water IR probe offers 26 and 34 times higher sensitivity and even 200 and 86 times lower detection limit for immunosensing and DNA sensing, respectively, as compared to the traditional IR molecular fingerprints.

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“…Generally, the characteristics of PiFM spectra are in good agreement with the macroscopic spectra. For MDHA, the absorption band at around 1710 cm -1 corresponds to the carbonyl stretch C=O 22 . For MBA, two characteristic peaks are observed with PiFM.…”

Section: Figures 3a and 3bmentioning

confidence: 99%

Tip-Enhanced Infrared Imaging with Sub-10 nm Resolution and Hypersensitivity

Jahng

Pang

et al. 2020

J. Phys. Chem. Lett.

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IR spectroscopy has been widely used for chemical identification and quantitative analysis of reactions occurring in a specific time and space domains by measuring an average signal of the entire system 1 . Achieving IR measurements with nanometer-scale spatial resolution is highly desirable to obtain a detailed understanding of the composition, structure and function of interfaces 2-5 . The challenges in IR nanoscopy yet exist owing to the small molecular cross section and pristine optical diffraction limit. Although atomic force microscopy (AFM) based techniques, such as scattering-type scanning near-field optical microscopy and photothermal-induced resonance microscopy (PTIR), can acquire IR spectroscopy in a few tens of nanometer scale resolution 6-9 , IR measurements with monolayer level sensitivity remains elusive and can only be realized under critical conditions 10,11 . Herein, we demonstrate sub-10 nm spatial resolution sampling a volume of ~360 molecules with a strong field enhancement at the sample-tip junction by implementing noble metal substrates (Au, Ag, Pt) in photo-induced force microscopy (PiFM). This technique shows versatility and robustness of PiFM, and is promising for application in interfacial studies with hypersensitivity and super spatial resolution.

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Chain-length dependent interfacial immunoreaction kinetics on self-assembled monolayers revealed by surface-enhanced infrared absorption spectroscopy

Cited by 10 publications

References 34 publications

Functional Nanomaterials on 2D Surfaces and in 3D Nanocomposite Hydrogels for Biomedical Applications

Functional Nanomaterials on 2D Surfaces and in 3D Nanocomposite Hydrogels for Biomedical Applications

Water as a Universal Infrared Probe for Bioanalysis in Aqueous Solution by Attenuated Total Reflection–Surface Enhanced Infrared Absorption Spectroscopy

Tip-Enhanced Infrared Imaging with Sub-10 nm Resolution and Hypersensitivity

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