Chain-length dependence of the protein and cell resistance of oligo(ethylene glycol)-terminated self-assembled monolayers on gold

Abstract

Oligo(ethylene glycol) (O-EG(n))-terminated alkanethiol surface-assembled monolayers (SAMs) have been reported to demonstrate protein-resistant properties similar to those of poly(ethylene glycol) (PEG). In this study, we compared the relative protein resistance of short and long ethylene oxide chains, SAMs of PEG 5000, PEG 2000, O-EG(3) (molecular weight = 120), and O-EG(6) (molecular weight = 240), on gold surfaces. Surface plasmon resonance showed that these monolayers were all protein-resistant within the uncertainty of the measurement. However, they exhibited different adhesive properties toward 3T3 mouse fibroblast adhesion in supplemented Dulbecco's modified Eagles medium. The results show that the cell adhesion was sensitive to the concentration of proteins supplemented in the culture medium and to the length of PEG chains.

Development of peristaltic antithrombogenic micropumps for in vitro and ex vivo blood transportation tests

“…The cleaned chip surface is hydrophilic, with a contact angle of 16°-22°. Following the coating process, the PEG and PEOU surfaces exhibited contact angles of 42°-46°and 48°-56°, respectively (not shown here), revealing results similar to those reported elsewhere (Lee and Laibinis 1998;Zhu et al 2001). The same terminal groups (OH for both PEG and PEOU) are associated with similar contact angles.…”

Steric Stabilizers for Cubic Phase Lyotropic Liquid Crystal Nanodispersions (Cubosomes)

“…PEG has been shown to be able to form a stealth corona around liposomes, significantly reducing the rapid uptake of intravenously injected particulate drug carriers by cells of the mononuclear phagocyte system (MPS) [132,133]. It has been demonstrated theoretically [134][135][136][137] and experimentally [138][139][140][141][142][143] that protein repellence of PEG coatings depends on both chain length and chain density, which jointly determine the thickness of the adlayer [127].…”

Polymer brushes and self-assembled monolayers: Versatile platforms to control cell adhesion to biomaterials (Review)

“…These studies showed that proteins are reversibly adsorbed; they are removed upon rinsing the substrate with water. 120 107 Since FN mediates cell adhesion to surfaces, quantifying the amount of FN on the surface and determining conditions under which this can be controlled provides a method to tailor the amount of cell adhesion to the surface. After incubation of mixed-alkanethiol SAMs on gold substrates in radiolabeled FN for 1 h the substrates were incubated in solutions of either phosphate buffered saline ͑PBS͒ or 10% newborn calf serum in Dulbecco's modified eagle's medium for 1 or 16 h, and the amount of FN on the surface was quantified.…”