Chain conformation of an acidic polysaccharide from green tea and related mechanism of α-amylase inhibitory activity

Yin, Lin; Fu, Shanshan; Wu, Roujun; Wei, Shuyue; Yi, Ju-Zhen; Zhang, Liming; Yang, Liqun

doi:10.1016/j.ijbiomac.2020.07.125

Cited by 28 publications

(9 citation statements)

References 53 publications

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“…Macromolecular characteristics of pectic-polysaccharides, such as molecular mass, molecular mass distribution, and particle size, have significant impacts on their biological properties and applications in the functional food industry ( 9 , 30 , 31 ). Consequently, macromolecular characteristics of OPP with various DE values were measured and compared.…”

Section: Resultsmentioning

confidence: 99%

Effects of various degrees of esterification on antioxidant and immunostimulatory activities of okra pectic-polysaccharides

Wang

et al. 2022

Front. Nutr.

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Pectic-polysaccharides are considered as one of the most abundant bioactive components in okra, which possess various promising health-promoting effects. However, the knowledge regarding the structure-bioactivity relationship of okra pectic-polysaccharides (OPP) is still limited. In this study, effects of various degrees of esterification (DEs) on in vitro antioxidant and immunostimulatory activities of OPP were analyzed. Results displayed that OPP with high (42.13%), middle (25.88%), and low (4.77%) DE values were successfully prepared by mild alkaline de-esterification, and their primary chemical structures (compositional monosaccharide and glycosidic linkage) and molecular characteristics (molecular weight distribution, particle size, and rheological property) were overall stable. Additionally, results showed that the notable decrease of DE value did not significantly affect antioxidant activities [2,2’-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) and nitric oxide (NO) radical scavenging abilities as well as ferric reducing antioxidant power (FRAP)] of OPP, suggesting that the DE was not closely related to its antioxidant activity. In fact, the slight decrease of antioxidant activity of OPP after the alkaline de-esterification might be attributed to the slight decrease of uronic acid content. Nevertheless, the immunostimulatory effect of OPP was closely related to its DE, and a suitable degree of acetylation was beneficial to its in vitro immunostimulatory effect. Besides, the complete de-acetylation resulted in a remarkable reduction of immune response. The findings are beneficial to better understanding the effect of DE value on antioxidant and immunomodulatory activities of OPP, which also provide theoretical foundations for developing OPP as functional foods or health products.

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Section: Resultsmentioning

confidence: 99%

Effects of various degrees of esterification on antioxidant and immunostimulatory activities of okra pectic-polysaccharides

Wang

et al. 2022

Front. Nutr.

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“…To test whether polysaccharides EPS-2 can interact with Aβ 1-42 , molecular docking simulation was applied to simulate, identify, and predict to construct polysaccharide-protein interaction systems, mainly using polysaccharide lower repeat units (Makshakova et al, 2021;L. Yin et al, 2020).…”

Section: Molecular Simulation Of Interactions Between Eps-2 and Aβ 1-42mentioning

confidence: 99%

Mannose‐rich exopolysaccharide from Agaricus sinodeliciosus var. Chaidam: Purification, chemical characterization, and neuroprotective activity

Chen

Shi

et al. 2023

Food Frontiers

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Amyloid β (Aβ) aggregation is a hallmark of Alzheimer's disease (AD). Searching for compounds that attenuate Aβ aggregation could uncover novel prevention for AD. Herein, an exopolysaccharide (EPS)‐2 was purified from the fermentation broth of Agaricus sinodeliciosus var. Chaidam. Morphology characterization, circular dichroism spectroscopy, and molecular docking demonstrated that EPS‐2 inhibited oligomer formation and remodeled the conformational conversion of Aβ1‐42, thus reducing the cytotoxicity and oxidative stress induced by Aβ1‐42 oligomers in vitro. Structural characterization revealed that the neuroprotective EPS‐2 with an average molecular weight of 18 kDa consisted of mannose, galactose, and a small proportion of rhamnose, glucose, fucose, ribose, glucuronic acid, and galactosamine. Glycosidic linkage and nuclear magnetic resonance analysis suggested that EPS‐2 has a backbone of →1)‐D‐Galp‐(6→, →1)‐D‐Manp‐(2, 6→, →1)‐D‐Galp‐(2→ and →1)‐t‐Manp with branched chains. Therefore, EPS‐2 was able to block Aβ1‐42 aggregation and reduced its neurotoxicity, and thus can be developed into a potential functional food ingredient for AD patients.

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“…Tea polysaccharide is a bioactive component in tea. The most important function of tea polysaccharide is hypoglycemic activity, which has been proved both in vitro ( 7 , 8 ) and in vivo ( 5 , 9 ). Additionally, tea polysaccharide has been found to exhibit numerous bioactivities, including anti-oxidation, anti-diabetic, anti-obesity, anti-tumor, and gut microbiota regulation effects ( 10 ).…”

Section: Introductionmentioning

confidence: 99%

Structural Characterization of a Low Molecular Weight HG-Type Pectin From Gougunao Green Tea

et al. 2022

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Tea is a popular beverage with a long history of safe and healthy use. Tea polysaccharide is a bioactive component extracted from tea, which has attracted more and more attention in recent decades. In this article, an acidic polysaccharide Gougunao tea polysaccharide (GPS) was isolated from Gougunao green tea by hot water extraction and ethanol precipitation. After purification by a diethylaminoethyl (DEAE) Sepharose Fast Flow column and a Sephacryl S-400 column, several homogalacturonan (HG) and rhamnogalacturonan-I (RG-I) fractions were obtained. Fraction GPS2b with the highest yield was selected for structural characterization by methylation and nuclear magnetic resonance (NMR) analysis. GPS2b was found to be an HG-type pectic polysaccharide (degree of methyl esterification [DE], 51.6%) with low molecular weight (Mw, 36.8 kDa). It was mainly composed of →4)-α-GalpA- (1→ and →4)-α-GalpA-6-OMe-(1→. In addition, a minor highly branched RG-I domain was identified in this fraction. The investigation of structural features of tea polysaccharides can provide insights to understand their structure-bioactivity relationship.

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Chain conformation of an acidic polysaccharide from green tea and related mechanism of α-amylase inhibitory activity

Cited by 28 publications

References 53 publications

Effects of various degrees of esterification on antioxidant and immunostimulatory activities of okra pectic-polysaccharides

Effects of various degrees of esterification on antioxidant and immunostimulatory activities of okra pectic-polysaccharides

Mannose‐rich exopolysaccharide from Agaricus sinodeliciosus var. Chaidam: Purification, chemical characterization, and neuroprotective activity

Structural Characterization of a Low Molecular Weight HG-Type Pectin From Gougunao Green Tea

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