Chain conformation and biological activities of hyperbranched fucoidan derived from brown algae and its desulfated derivative

Wei, Xueqin; Cai, Liqin; Liu, Hailing; Tu, Honglei; Xu, Xiaojuan; Zhou, Fuling; Zhang, Lina

doi:10.1016/j.carbpol.2018.12.060

Cited by 50 publications

(17 citation statements)

References 53 publications

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“…37 The different chain conformations of polysaccharides usually exhibit diverse bioactivities. 38,39 AFM and DLS were employed to investigate the chain conformation of CA in the aqueous solution. The AFM image showed that CA exhibited a spherelike chain conformation in the aqueous solution with an average particle size of 286.44 nm (Figure 5D).…”

Section: Optimization Of the Submerged Fermentationmentioning

confidence: 99%

Colanic Acid: Biosynthetic Overproduction by Engineering Escherichia coli and Physical Property Characterization

Qiao

Zhan

Tan

et al. 2021

J. Agric. Food Chem.

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Colanic acid has promising applications in food, cosmetic, and healthcare fields. In this study, a recombinant WQM003/pRAU was constructed by deleting genes lon and hns and overexpressing genes rcsA and galU in E. coli MG1655Δ(L-Q). After systematic optimization of fermentation conditions, colanic acid yield in WQM003/pRAU reached 19.79 g/L, the highest yield reported so far. The colanic acid produced by WQM003/pRAU was purified and its structure and physical properties were determined. This colanic acid shows a triple-helical structure and is stable up to 102 °C, and its melting temperature is 253.9 °C. This colanic acid shows a sphere-like chain conformation in aqueous solution. The viscosity of this colanic acid solution is related to concentration, shear rate, salt, temperature, and pH. At high concentrations, this colanic acid shows both viscous and elastic behaviors. These results suggest that the colanic acid produced by WQM003/pRAU has broad application prospects.

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Section: Optimization Of the Submerged Fermentationmentioning

confidence: 99%

Colanic Acid: Biosynthetic Overproduction by Engineering Escherichia coli and Physical Property Characterization

Qiao

Zhan

Tan

et al. 2021

J. Agric. Food Chem.

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“…Sulfate esters do have a noticeable effect on several properties, including physicochemical ones. Wei et al, found that decreasing the amount of ester sulfate groups in L. Japonica fucoidan increased aggregate formation, due to an abundance of inter-chain hydrogen bonds [ 10 ]. Such conformational changes are less studied, even though they may affect polymer activity in vivo .…”

Section: Introductionmentioning

confidence: 99%

Complementarity of Raman and Infrared spectroscopy for rapid characterization of fucoidan extracts

et al. 2021

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Background Fucoidans are sulfated polysaccharides from the cell-wall of brown algae. They have a wide range of applications in medicine, including regenerative medicine, ophthalmology, cancer, and autoimmune disease. Biological activity of fucoidans directly depends on their structure, which remains poorly understood. This is primarily because the polymeric nature of these molecules limits the use of nuclear magnetic resonance and mass spectrometry, classical tools of structural biology for their structural characterization. Raman and Infrared spectroscopies are non-invasive and non-destructive techniques that can be used to probe the structural organization of biological specimens. In this study, we investigate the potential of Raman and Infrared spectroscopy for structural analysis of several fucoidan extracts. Results Our results show that Infrared and Raman provide different but complimentary information about the structure of crude extracts of fucoidans, revealing the presence of minor impurities from co-extractants. We also found that at high extraction temperatures acidic conditions limit formation of melanoidins, while also yielding relatively high sulfate ester fucoidan. However, at high temperatures, water extraction may potentially result in formation of advanced glycation end products. Their presence could be problematic for fucoidan extracts intended for medicinal use, as advanced glycation end products have been linked to endocrine interruption mechanisms in vivo by crosslinking to and permanently altering extracellular matrix proteins. Conclusion Raman and Infrared can be used as complementary tools for rapid screening of crude fucoidan extracts, which can be a valuable tool for assessing impurities that remain after extraction.

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“…It was also supported by the an inhibition of tubulogenesis in rabbit aorta endothelial cells [21]. These results are confirmed by the antiangiogenic properties of SPs from Laminaria japonica [22] and Sargassum fusiforme [23] on the in vitro angiogenesis model where formation of tubules were inhibited in both species. Angiogenesis plays an important role in tumor growth, metastasis and inhibiting vascular growth as these abnormalities can lead to a positive impact in people with tumors.…”

Section: Pharmacologic Potential Activities Of Spsmentioning

confidence: 65%

Pharmacologic Application Potentials of Sulfated Polysaccharide from Marine Algae

Manlusoc

Hsieh

et al. 2019

Polymers

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With the advent of exploration in finding new sources for treating different diseases, one possible natural source is from marine algae. Having an array of potential benefits, researchers are interested in the components which comprise one of these activities. This can lead to the isolation of active compounds with biological activities, such as antioxidation of free radicals, anti-inflammation, antiproliferation of cancer cells, and anticoagulant to name a few. One of the compounds that are isolated from marine algae are sulfated polysaccharides (SPs). SPs are complex heterogenous natural polymers with an abundance found in different species of marine algae. Marine algae are known to be one of the most important sources of SPs, and depending on the species, its chemical structure varies. This variety has important physical and chemical components and functions which has gained the attention of researchers as this contributes to the many facets of its pharmacologic activity. In this review, recent pharmacologic application potentials and updates on the use of SPs from marine algae are discussed.

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Chain conformation and biological activities of hyperbranched fucoidan derived from brown algae and its desulfated derivative

Cited by 50 publications

References 53 publications

Colanic Acid: Biosynthetic Overproduction by Engineering Escherichia coli and Physical Property Characterization

Colanic Acid: Biosynthetic Overproduction by Engineering Escherichia coli and Physical Property Characterization

Complementarity of Raman and Infrared spectroscopy for rapid characterization of fucoidan extracts

Pharmacologic Application Potentials of Sulfated Polysaccharide from Marine Algae

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