Chagas' Disease: Serodiagnosis with Purified Gp25 Antigen

Scharfstein, Júlio; Luquetti, Alejandro Ostermayer; Murta, A. C. M.; Senna, Mônica Carneiro Alves; Rezende, Joffre M.; Rassi, Anis; Mendonça-Previato, Lúcia

doi:10.4269/ajtmh.1985.34.1153

Cited by 47 publications

(27 citation statements)

References 6 publications

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“…This result agrees with data reported previously, indicating 100% reactivity with the same protein. 12 Another T. cruzi protein, gp 25,27 was recognized by 55.14% of the serum samples from Group I, and was not recognized by serum samples from Groups III or IV, which confirms its usefulness for the immunodiagnosis of Chagas' disease. Different results were obtained by Grijalva and others with ELISA-reactive samples from patients with Chagas' disease.…”

Section: Discussionmentioning

confidence: 90%

“…Although the World Health Organization recommends three different serologic methods to establish the presence of T. cruzi infection in humans, it is difficult to use this procedure for routine screening of blood donors. 27 Brazilian law requires the use of two serologic techniques, chosen from among available recommended tests such as the IFA, IHA, DA, CF, or ELISA, 4 for screening blood donors. When one considers the low prevalence of Chagas' disease among blood donors, the occurrence of a large number of dubious and false-positive results for Chagas' disease in the initial screening is expected.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of the western blot in the confirmatory serologic diagnosis of Chagas' disease.

Reiche

Cavazzana²,

Okamura³

et al. 1998

The American Journal of Tropical Medicine and Hygiene

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Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Evaluation of the western blot in the confirmatory serologic diagnosis of Chagas' disease.

Reiche

Cavazzana²,

Okamura³

et al. 1998

The American Journal of Tropical Medicine and Hygiene

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“…In the last years there have been intensive researches on more defined and purified antigens for the diagnosis of T. cruzi infection 1,62,67,71 . Due to the high complexity of the molecular constitution and antigenic composition of that parasite, it became clear that single antigens lack the required sensitivity when compared with conventional tests 71 .…”

Section: Discussionmentioning

confidence: 99%

Long term evaluation of etiological treatment of Chagas disease with benznidazole

Cançado

2002

Rev. Inst. Med. trop. S. Paulo

273

175

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The aim of this article is to present an investigation of cure rate, after long follow up, of specific chemotherapy with benznidazole in patients with both acute and chronic Chagas disease, applying quantitative conventional serological tests as the base of the criterion of cure. Twenty one patients with the acute form and 113 with one or other of the various chronic clinical forms of the disease were evaluated, after a follow up period of 13 to 21 years, for the acute, and 6 to 18 years, for the chronic patients. The duration of the acute as well as the chronic disease, a condition which influences the results of the treatment, was determined. The therapeutic schedule was presented, with emphasis on the correlation between adverse reactions and the total dose of 18 grams, approximately, as well as taking into consideration precautions to assure the safety of the treatment. Quantitative serological reactions consisting of complement fixation, indirect immunofluorescence, indirect hemagglutination, and, occasionally, ELISA, were used. Cure was found in 76 per cent of the acute patients but only in 8 per cent of those with chronic forms of the disease. In the light of such contrasting results, fundamentals of the etiological therapy of Chagas disease were discussed, like the criterion of cure, the pathogenesis and the role of immunosuppression showing tissue parasitism in long standing chronic disease, in support of the concept that post-therapeutic consistently positive serological reactions mean the presence of the parasite in the patient's tissues. In relation to the life-cycle of T. cruzi in vertebrate host, there are still some obscure and controversial points, though there is no proof of the existence of resistant or latent forms. However, the finding over the last 15 years, that immunosuppression brings about the reappearance of acute disease in long stand chronic patients justifies a revision of the matter. Facts were quoted in favor of the treatment of chronic patients.

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“…The enzyme is found in all developmental forms of different T. cruzi isolates, with levels 10-fold higher in epimastigotes and is active in the pH range of 5.0 to 7.5 [58][59][60]. Cruzipain has an N-terminal catalytic domain linked to an antigenic C-terminal extension, the gp25 [61,62]. Structural characterization of the oligosaccharide chains revealed the presence of sulfated residues that are essential for cruzipain recognition by IgG antibodies from the serum of chagasic patients [63,64].…”

Section: Family C1 (Cruzipain and Others)mentioning

confidence: 99%

Biological Roles of Peptidases in Trypanosomatids~!2009-11-26~!2010-02-15~!2010-03-18~!

Vermelho

Branquinha²,

d’Avila-Levy³

et al. 2010

TOPARAJ

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Abstract:In this review, we report the recent developments in the characterization of peptidases and their possible biological functions in the Trypanosomatidae family. The focus will be on peptidases from Trypanosoma cruzi, Leishmania spp., African trypanosomes and plant and insect trypanosomatids. There are numerous events in parasite development where the involvement of peptidases has been established, and they will be approached in the present review. Also in this review we will discuss the central roles have been proposed for peptidases in diverse processes such as virulence, host cell interaction and invasion, catabolism of host proteins, differentiation, cell cycle progression and both stimulation and evasion of host immune responses.

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Chagas' Disease: Serodiagnosis with Purified Gp25 Antigen

Cited by 47 publications

References 6 publications

Evaluation of the western blot in the confirmatory serologic diagnosis of Chagas' disease.

Evaluation of the western blot in the confirmatory serologic diagnosis of Chagas' disease.

Long term evaluation of etiological treatment of Chagas disease with benznidazole

Biological Roles of Peptidases in Trypanosomatids~!2009-11-26~!2010-02-15~!2010-03-18~!

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