Chagas' Disease: IgG Isotypes against Trypanosoma cruzi Cytosol Acidic Antigens in Patients with Different Degrees of Heart Damage

Immunological imbalance between IFN-³ and IL-10 levels in the sera of patients with the cardiac form of Chagas disease

“…The current results, using total epimastigote antigen, showed high levels of IgG1 in chagasic patients compared to non-chagasic individuals and similar IgG1 levels in with the IND versus the CARD clinical forms. These results are in agreement with data that demonstrated high IgG1 concentrations against epimastigote antigen in sera from all groups of chagasic pa- tients by ELISA, regardless of the clinical form (Cerban et al 1993). High IgG1 and similar IgG3 production in patients presenting different clinical forms of the disease were described using different antigenic preparations, such as paraflagellar rod protein (Michailowsky et al 2003).…”

“…High IgG1 and similar IgG3 production in patients presenting different clinical forms of the disease were described using different antigenic preparations, such as paraflagellar rod protein (Michailowsky et al 2003). Cerban et al (1993) observed no significant differences regarding IgG isotype production in chronic chagasic patients with different clinical forms. However, a different antigenic recognition pattern by IgG1 among different clinical groups by immunoblotting using acidic antigenic fraction (AAF) separated from T. cruzi cytosol was observed.…”

“…Correlation between the different clinical forms of Chagas disease and the levels of IgG isotypes has not been clearly defined. Several authors have tried to demonstrate the correlation between IgG isotypes and the severity of the different clinical forms of Chagas disease both in patient groups (Cerban et al 1993, Morgan et al 1996, Michailowsky et al 2003 and in experimental models (Giordanengo et al 2000, Guedes et al 2008. While some studies detected no differences between the levels of these immunoglobulins (IgG) among individuals with different clinical manifestations (Cerban et al 1993, Michailowsky et al 2003, others demonstrated higher levels of IgG2 antibodies in the sera of patients with cardiac and digestive manifestations of the disease (Morgan et al 1996, Cordeiro et al 2001.…”

Electrocardiographic findings in Mexican chagasic subjects living in high and low endemic regions of Trypanosoma cruzi infection

“…It is also known that chronic chagasic cardiomyopathy patients display high anti-T. cruzi antibodies even in those infected cases that lived in endemic zones for only short time of their life (Cerban et al 1993, Umezawa et al 1996). This conflicting point deserves further analysis to explain why antibody levels could be different between resident from high and low endemic regions in spite of parasite persistence in both groups.…”

In-house ELISA method to analyze anti-Trypanosoma cruzi IgG reactivity for differential diagnosis and evaluation of Chagas disease morbidity

“…Several authors have already studied the relationship between the intensity of serological reactivity of different IgG subclasses and Chagas disease morbidity [7][8][9][10][11][12] . Morgan et al detected increased levels of IgG2 in CARD patients using an ELISA test based on epimastigotes of the Brazil strain.…”