Chagas’ disease: application of TESA‐blot in inconclusive sera from a Brazilian blood bank

Silveira-Lacerda, Elisângela de Paula; Silva, A. G.; Junior, S. F.; Souza, Maria Aparecida de; Kesper, Norival; Botelho-Filho, A.; Umezawa, Eufrosina Setsu

doi:10.1111/j.1423-0410.2004.00571.x

Cited by 37 publications

(39 citation statements)

References 12 publications

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“…In immunosuppressed patients posttransplantation, discordant results using different serological techniques have been reported (5,104,310), and this has also been noted in patients with viral infections and AIDS. Although not specifically used in immunocompromised patients, new techniques using T. cruzi excretedsecreted antigens are under development (416) Tests based on T. cruzi excreted-secreted antigens (ELISA and WB) have shown high sensitivity and specificity in the diagnosis of acute and chronic infection by T. cruzi (373), without cross-reactivity with other diseases (341). In order to confirm infection with T. cruzi, the WHO recommends a minimum of two positive serological tests using different methods and different antigens.…”

Section: Diagnostic Methods (I) Parasitological Methodsmentioning

confidence: 99%

Transmission of Tropical and Geographically Restricted Infections during Solid-Organ Transplantation

et al. 2008

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SUMMARY In recent years, the increasing number of donors from different regions of the world is providing a new challenge for the management and selection of suitable donors. This is a worldwide problem in most countries with transplantation programs, especially due to the increase in immigration and international travel. This paper elaborates recommendations regarding the selection criteria for donors from foreign countries who could potentially transmit tropical or geographically restricted infections to solid-organ transplant recipients. For this purpose, an extensive review of the medical literature focusing on viral, fungal, and parasitic infections that could be transmitted during transplantation from donors who have lived or traveled in countries where these infections are endemic has been performed, with special emphasis on tropical and imported infections. The review also includes cases described in the literature as well as risks of transmission during transplantation, microbiological tests available, and recommendations for each infection. A table listing different infectious agents with their geographic distributions and specific recommendations is included.

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Section: Diagnostic Methods (I) Parasitological Methodsmentioning

confidence: 99%

Transmission of Tropical and Geographically Restricted Infections during Solid-Organ Transplantation

et al. 2008

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“…The IHA is also used for screening and, on occasions, as a second test for confirmation. However, sensitivity and specificity of commercial reagents available varies widely (Saez-Alquezar et al 1997) other non conventional tests that has been used for confirmation are an Immunoblot (Umezawa et al 1996), the TESA (Silveira-Lacerda et al 2004), the INNOLIA Chagas (Saez-Alquezar et al 2000), and the RIPA (Leiby et al 2000), all aimed at replacing the IIF as the gold standard.…”

Section: Preventionmentioning

confidence: 99%

Epidemiology of Chagas disease in non endemic countries: the role of international migration

Schmuñis

2007

Mem. Inst. Oswaldo Cruz

473

290

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“…The status of some individuals remains difficult to resolve even after a third test, because there is no true gold standard assay for chronic T. cruzi infection (283). Assays such as the radioimmunoprecipitation assay (RIPA) and trypomastigote excreted-secreted antigen immunoblot (TESA-blot) are promoted as reference tests, but even these do not have perfect sensitivity and specificity and may not be capable of resolving the diagnosis (168,272).…”

Section: Diagnosis Of Chronic T Cruzi Infectionmentioning

confidence: 99%

Trypanosoma cruzi and Chagas' Disease in the United States

et al. 2011

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SUMMARY Chagas' disease is caused by the protozoan parasite Trypanosoma cruzi and causes potentially life-threatening disease of the heart and gastrointestinal tract. The southern half of the United States contains enzootic cycles of T. cruzi , involving 11 recognized triatomine vector species. The greatest vector diversity and density occur in the western United States, where woodrats are the most common reservoir; other rodents, raccoons, skunks, and coyotes are also infected with T. cruzi. In the eastern United States, the prevalence of T. cruzi is highest in raccoons, opossums, armadillos, and skunks. A total of 7 autochthonous vector-borne human infections have been reported in Texas, California, Tennessee, and Louisiana; many others are thought to go unrecognized. Nevertheless, most T. cruzi -infected individuals in the United States are immigrants from areas of endemicity in Latin America. Seven transfusion-associated and 6 organ donor-derived T. cruzi infections have been documented in the United States and Canada. As improved control of vector- and blood-borne T. cruzi transmission decreases the burden in countries where the disease is historically endemic and imported Chagas' disease is increasingly recognized outside Latin America, the United States can play an important role in addressing the altered epidemiology of Chagas' disease in the 21st century.

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Chagas’ disease: application of TESA‐blot in inconclusive sera from a Brazilian blood bank

Cited by 37 publications

References 12 publications

Transmission of Tropical and Geographically Restricted Infections during Solid-Organ Transplantation

Transmission of Tropical and Geographically Restricted Infections during Solid-Organ Transplantation

Epidemiology of Chagas disease in non endemic countries: the role of international migration

Trypanosoma cruzi and Chagas' Disease in the United States

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