Chagas Disease After Organ Transplantation - Los Angeles, California, 2006

Mascola, Laurene; Kubak, B.; Radhakrishna, Suman; Mone, Tom; Hunter, Robert N.; Leiby, David A.; Kuehnert, Matthew J.; Moore, Anne; Steurer, Frank; Lawrence, Gena G.; Kun, Heather

doi:10.1037/e523792006-003

Cited by 6 publications

(6 citation statements)

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“…Another two patients who received heart transplant were also infected, but in both cases recipients of other organs from the same donor were apparently not infected (Mascola et al 2006).…”

Section: Discussionmentioning

confidence: 99%

Epidemiology of Chagas disease in non endemic countries: the role of international migration

Schmuñis

2007

Mem. Inst. Oswaldo Cruz

481

297

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“…Another two patients who received heart transplant were also infected, but in both cases recipients of other organs from the same donor were apparently not infected (Mascola et al 2006).…”

Section: Discussionmentioning

confidence: 99%

Epidemiology of Chagas disease in non endemic countries: the role of international migration

Schmuñis

2007

Mem. Inst. Oswaldo Cruz

481

297

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“…Also important to note is that rural areas of Mexico and Central and South America represent the greatest risk of infection acquisition and travel to urban or metropolitan areas in countries in this region does not confer risk of acquiring T. cruzi infection. Attention is now focused on human‐to‐human transmission by transfusion, 7 organ transplant, 8,9 or vertically, from mother to child 10 . Enzootic evidence of T. cruzi in the United States coupled with immigration from endemic regions highlights the need to further characterize the prevalence of T. cruzi infection and particularly the threat to the blood supply 11,12 .…”

mentioning

confidence: 99%

Epidemiologic and laboratory findings from 3 years of testing United States blood donors for Trypanosoma cruzi

et al. 2012

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“…T. cruzi can also be transmitted by transplantation of organs obtained from chronically infected persons. Numerous instances of such transmission have been reported in the countries in which the parasite is endemic, and five cases have occurred in the United States (18,29).…”

mentioning

confidence: 99%

Immunoblot Assay Using Recombinant Antigens as a Supplemental Test To Confirm the Presence of Antibodies to Trypanosoma cruzi

Cheng

Chang

Salbilla

et al. 2007

Clin Vaccine Immunol

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The diagnosis of chronic Chagas' disease is generally made by detecting antibodies to Trypanosoma cruzi. Most conventional serological tests are based on lysates of whole parasites or semipurified antigen fractions from T. cruzi epimastigotes grown in culture. The occurrence of inconclusive and false-positive results has been a persistent problem with the conventional assays, and there is no universally accepted gold standard for confirmation of positive test results. We describe here an immunoblot assay for detecting antibodies to T. cruzi in which four chimeric recombinant antigens (rAgs), designated FP3, FP6, FP10, and TcF, are used as target antigens. Each of these rAgs is composed of several antigenically distinct regions and includes repetitive as well as nonrepetitive sequences. Each rAg is coated as a discrete line on a nitrocellulose strip. Assay sensitivity was assessed by testing 345 specimens known to be positive for antibodies to T. cruzi. All 345 of these samples showed two to four reactive test bands in addition to the three on-board control bands that are on each strip. Assay specificity was determined by testing 500 specimens from random U.S. blood donors, all of which gave negative results. Based on the results obtained in this study, we propose the following scheme for interpretation of test results: (i) no bands or a single test band ‫؍‬ a negative result; (ii) two or more test bands with at least one band showing intensity of 1؉ or higher ‫؍‬ a positive result; and (iii) multiple faint test bands (؎) ‫؍‬ indeterminate result. Based on this scheme, the prototype immunoblot assay showed sensitivity of 100% (n ‫؍‬ 345) and specificity of 100% (n ‫؍‬ 500). Additionally, all 269 potentially cross-reacting and T. cruzi antibodynegative specimens tested negative in our immunoblot assay. The rAg-based immunoblot assay has potential as a supplemental test for confirming the presence of antibodies to T. cruzi in blood specimens and for identifying false-positive results obtained with other assays.

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Chagas Disease After Organ Transplantation - Los Angeles, California, 2006

Cited by 6 publications

References 0 publications

Epidemiology of Chagas disease in non endemic countries: the role of international migration

Epidemiology of Chagas disease in non endemic countries: the role of international migration

Epidemiologic and laboratory findings from 3 years of testing United States blood donors for Trypanosoma cruzi

Immunoblot Assay Using Recombinant Antigens as a Supplemental Test To Confirm the Presence of Antibodies to Trypanosoma cruzi

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