Chaetoglobosins and azaphilones from Chaetomium globosum associated with Apostichopus japonicus

Qi, Jun; Jiang, Lan; Zhao, Peipei; Chen, Haiyan; Jia, Xiaopeng; Zhao, Liya; Dai, Huanqin; Hu, Jiansen; Liu, Changheng; Shim, Sang Hee; Xia, Xuekui; Zhang, Lixin

doi:10.1007/s00253-019-10308-0

Cited by 17 publications

(27 citation statements)

References 25 publications

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“…The involvement of the transposase in chaetoglobosin biosynthesis has not been demonstrated to date. However, the absence of the gene CHGG-01238 in another chaetoglobosin-producing strain of C. globosum suggests that it is not essential for the synthesis of these compounds [ 94 ]. Our transcription analyses (microarray and qPCR) showed that all genes of this putative gene cluster were over-expressed in PeΔ brlA .…”

Section: Discussionmentioning

confidence: 99%

The brlA Gene Deletion Reveals That Patulin Biosynthesis Is Not Related to Conidiation in Penicillium expansum

Zetina-Serrano

Rocher

Naylies

et al. 2020

IJMS

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Dissemination and survival of ascomycetes is through asexual spores. The brlA gene encodes a C2H2-type zinc-finger transcription factor, which is essential for asexual development. Penicillium expansum causes blue mold disease and is the main source of patulin, a mycotoxin that contaminates apple-based food. A P. expansum PeΔbrlA deficient strain was generated by homologous recombination. In vivo, suppression of brlA completely blocked the development of conidiophores that takes place after the formation of coremia/synnemata, a required step for the perforation of the apple epicarp. Metabolome analysis displayed that patulin production was enhanced by brlA suppression, explaining a higher in vivo aggressiveness compared to the wild type (WT) strain. No patulin was detected in the synnemata, suggesting that patulin biosynthesis stopped when the fungus exited the apple. In vitro transcriptome analysis of PeΔbrlA unveiled an up-regulated biosynthetic gene cluster (PEXP_073960-PEXP_074060) that shares high similarity with the chaetoglobosin gene cluster of Chaetomium globosum. Metabolome analysis of PeΔbrlA confirmed these observations by unveiling a greater diversity of chaetoglobosin derivatives. We observed that chaetoglobosins A and C were found only in the synnemata, located outside of the apple, whereas other chaetoglobosins were detected in apple flesh, suggesting a spatial-temporal organization of the chaetoglobosin biosynthesis pathway.

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Section: Discussionmentioning

confidence: 99%

The brlA Gene Deletion Reveals That Patulin Biosynthesis Is Not Related to Conidiation in Penicillium expansum

Zetina-Serrano

Rocher

Naylies

et al. 2020

IJMS

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“…In studies on microorganisms associated with sea cucumbers, samples are primarily obtained from the following body parts: the body wall [ 22 , 23 ], body surface [ 18 , 21 , 24 , 25 , 26 , 27 , 28 , 29 ], inner body tissue [ 30 ], coelomic fluid [ 24 , 31 ], stomach [ 30 ], intestines [ 4 , 6 , 17 , 19 , 25 , 32 , 33 , 34 , 35 ], brown gastrointestinal tissue [ 30 ], and feces [ 20 , 22 ].…”

Section: Microorganisms Associated With Sea Cucumbersmentioning

confidence: 99%

“…There are some papers on the diversity of culturable bacteria associated with sea cucumbers, which plays a very important role in understanding the digestion and diseases of sea cucumbers [ 4 , 6 , 17 , 25 , 33 ]. Because marine-derived fungi had shown potential to synthesize pharmaceutical compounds with bioactivities, researchers usually directly isolate fungi associated with sea cucumbers for the separation of active natural products [ 21 , 28 , 29 ], except one paper about the diversity and bioactivity of fungi associated with sea cucumbers [ 22 ].…”

Section: Microorganisms Associated With Sea Cucumbersmentioning

confidence: 99%

“…japonicus , on Chengshantou Island, China [ 29 ]. Compound 43 has some inhibitory effects against Staphylococcus aureus and methicillin-resistant Staphylococcus aureus (MRSA), with MIC values of 47.3 and 94.6 μM, respectively, and weak activity against Candida albicans SC5314, Candida albicans 17#, Pseudomonas aeruginosa , and Bacillus Calmette–Guérin (BCG), with MIC values >100 μg/mL for all organisms [ 29 ].…”

Section: Structures and Bioactivities Of Natural Productsmentioning

confidence: 99%

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Culturable Microorganisms Associated with Sea Cucumbers and Microbial Natural Products

et al. 2021

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Sea cucumbers are a class of marine invertebrates and a source of food and drug. Numerous microorganisms are associated with sea cucumbers. Seventy-eight genera of bacteria belonging to 47 families in four phyla, and 29 genera of fungi belonging to 24 families in the phylum Ascomycota have been cultured from sea cucumbers. Sea-cucumber-associated microorganisms produce diverse secondary metabolites with various biological activities, including cytotoxic, antimicrobial, enzyme-inhibiting, and antiangiogenic activities. In this review, we present the current list of the 145 natural products from microorganisms associated with sea cucumbers, which include primarily polyketides, as well as alkaloids and terpenoids. These results indicate the potential of the microorganisms associated with sea cucumbers as sources of bioactive natural products.

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“…Natural products from plant-associated fungi are a rich source of biologically promising lead compounds. The fungus genus Chaetomium is the rich source of cytochalasans, among which Chaetomium globosum can produce plenty of such secondary metabolites [ 1 , 2 , 3 , 4 , 5 ]. As the main secondary metabolites from C. globosum , cytochalasans are a class of compounds containing isoindolinone moieties fused to the tricyclic or tetracyclic ring systems, which have a variety of pharmacological activities, such as antitumor [ 6 ], antibacterial [ 7 ], immunomodulatory [ 8 ], and phytotoxicity [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

New Cytotoxic Cytochalasans from a Plant-Associated Fungus Chaetomium globosum kz-19

Wang

et al. 2021

Marine Drugs

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Four new cytochalasans, phychaetoglobins A–D (1–4), together with twelve known cytochalasans (5–16), were isolated from a mangrove-associated fungus Chaetomium globosum kz-19. The new structures were elucidated on the basis of extensive 1D and 2D NMR, HR ESIMS spectroscopic analyses, and electronic circular dichroism (ECD) calculations. The absolute configuration of 2 was established by application of Mosher’s method. Compounds 4–8 exhibited moderate cytotoxicities against A549 and HeLa cell lines with the IC50 values less than 20 μM.

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Chaetoglobosins and azaphilones from Chaetomium globosum associated with Apostichopus japonicus

Cited by 17 publications

References 25 publications

The brlA Gene Deletion Reveals That Patulin Biosynthesis Is Not Related to Conidiation in Penicillium expansum

The brlA Gene Deletion Reveals That Patulin Biosynthesis Is Not Related to Conidiation in Penicillium expansum

Culturable Microorganisms Associated with Sea Cucumbers and Microbial Natural Products

New Cytotoxic Cytochalasans from a Plant-Associated Fungus Chaetomium globosum kz-19

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