CgGH insertion functional domain analysis in transgenic G1 and G2 and G3 mutiara catfish (Clarias gariepinus) broodstock

Abstract

Catfish is one of the most important freshwater fish farming commodities in Indonesia. Higher catfish production can be achieved by cultivating transgenic catfish carrying the growth hormone (GH) gene of African catfish (Clarias gariepinus GH, CgGH). This research focuses on analysis of the presence of the CgGH gene in transgenic G 1 , G 2 , and G 3 mutiara catfish broodstock, as an indication of stable CgGH inheritance. CgGH gene was isolated using the RNeasy mini kit and RT-PCR. RT-PCR revealed amplicons measuring approximately 600 bp in transgenic G 0 , G 1 , G 2 , and G 3 mutiara catfish. The CgGH consensus sequence similarities ranged from 93.76% to 97.06%, with four functional domain sites (somatotropin-1, somatotropin-2, four α-helix, N-glycosylation, four cysteine residues) of fish GH proteins. The functional domains of fish GH proteins are conserved in G 1 , G 2 , and G 3 and indicate stable exogenous GH inheritance to produce transgenic catfish strains in each generation.

Perbaikan Sistem Budikdamber Pemeliharaan Benih Ikan Lele Mutiara Padjadjaran

“…Penggunaan benih ikan unggul sangat diperlukan dalam pemeliharaan ikan dalam sistem tertutup ini (close system), mengingat kualitas genetik ikan dalam lingkungan pemeliharaan ekstrem menjadi kunci keberlanjutan budidaya. Keunggulan genetis ikan lele mutiara Padjadjaran terletak pada karakter pertumbuhan ikan dapat mencapai 2 kali lipat pertumbuhan ikan lele normal karena mengandung sisipan gen hormon pertumbuhan ikan lele dumbo (Clarias gariepinus growth hormone, CgGH) yang ditransfer ke dalam genom ikan lele mutiara (Buwono et al, 2019a, Buwono et al, 2022. Stabilitas peningkatan pertumbuhan dari generasi ke generasi pada ikan lele mutiara transgenik-GH (lele mutiara Padjadjaran) ini konsisten dengan peningkatan transmisi gen GH sisipan (CgGH) masingmasing 42% (G1), 50% (G2), 70% (G3) dan 80% (G4) (Gambar 1).…”