CFP10 and ESAT6 aptamers as effective Mycobacterial antigen diagnostic reagents

Tang, Xiaolei; Zhou, Ya-Xiong; Wu, Simin; Pan, Qin; Xia, Bing; Zhang, Xiaolian

doi:10.1016/j.jinf.2014.05.015

Cited by 62 publications

(36 citation statements)

References 49 publications

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“…Similarly, Tang et al (2014) selected aptamers for CFP-10 and ESAT-6, which were able to detect these proteins in serum. The authors suggest that these aptamers have a broad range of application, as they were able to detect CFP10 and ESAT6 in active pulmonary TB patients, extrapulmonary TB patients and healthy donors by using an enzyme-linked oligonucleotide assay (Tang et al 2014).…”

Section: Bacteriamentioning

confidence: 99%

Application of aptamers in diagnostics, drug-delivery and imaging

et al. 2016

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Aptamers are small, single-stranded oligonucleotides (DNA or RNA) that bind to their target with high specificity and affinity. Although aptamers are analogous to antibodies for a wide range of target recognition and variety of applications, they have significant advantages over antibodies. Since aptamers have recently emerged as a class of biomolecules with an application in a wide array of fields, we need to summarize the latest developments herein. In this review we will discuss about the latest developments in using aptamers in diagnostics, drug delivery and imaging. We begin with diagnostics, discussing the application of aptamers for the detection of infective agents itself, antigens/ toxins (bacteria), biomarkers (cancer), or a combination. The ease of conjugation and labelling of aptamers makes them a potential tool for diagnostics. Also, due to the reduced off-target effects of aptamers, their use as a potential drug delivery tool is emerging rapidly. Hence, we discuss their use in targeted delivery in conjugation with siRNAs, nanoparticles, liposomes, drugs and antibodies. Finally, we discuss about the conjugation strategies applicable for RNA and DNA aptamers for imaging. Their stability and self-assembly after heating makes them superior over protein-based binding molecules in terms of labelling and conjugation strategies.

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Section: Bacteriamentioning

confidence: 99%

Application of aptamers in diagnostics, drug-delivery and imaging

et al. 2016

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“…11 M. tuberculosis secreted protein CFP10 and ESAT-6 also confer strong cell-mediated immunity through T-cell proliferation and IFN-g production, which has been considered to be effective mycobacterial antigen diagnostic reagents. 22 Meanwhile, CFP10 and ESAT-6 are defective in BCG, and the virulence and immunogenicity of BCG could be enhanced by incorporation of RD1 region to restore the expression of CFP10 and ESAT6. 23 Besides, many immune regulatory cytokines also has been used in establishment of novel rBCGs due to their ability on improvement of T cell immunity.…”

Section: Discussionmentioning

confidence: 99%

Immunogenicity and protective efficacy of recombinant Bacille Calmette-Guerin strains expressing mycobacterium antigens Ag85A, CFP10, ESAT-6, GM-CSF and IL-12p70

Dai

Wang

Gong

et al. 2017

Human Vaccines & Immunotherapeutics

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“…Early secretory antigenic target 6 (ESAT-6) and culture filtrate protein 10 (CFP-10) are low-molecularweight proteins that are respectively encoded by the Rv3874 and Rv3875 genes located in the region of difference-1 in the genome of virulent MTB strains [11,12]. The ESAT-6 and the CFP-10 proteins can induce the malignant spread of MTB by participating in the cytolysis of alveolar epithelial cells and macrophages [13].…”

Section: Introductionmentioning

confidence: 99%

Early secretory antigenic target 6 and culture filtrate protein 10 as diagnostic indicators in IgA nephropathy associated with renal tuberculosis

Fang¹,

You²,

Wang³

et al. 2020

Preprint

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Background: This study evaluated the diagnostic value of early secretory antigenic target 6 (ESAT-6) and culture filtrate protein 10 (CFP-10) in immunoglobulin A nephropathy (IgAN) associated with renal tuberculosis (RT).Methods: Between January 2013 and January 2016, 40 patients with IgAN (IgAN group), 32 patients with RT (RT group), and 52 patients with IgAN associated with RT (IgAN + RT group) were selected for this study. A Tuberculin skin test (TST) was conducted, and serum Mycobacterium tuberculosis (MTB) antibody levels were measured. Urine samples were collected to culture MTB. Immunohistochemistry and western blotting were used to determine the expression of ESAT-6 and CFP-10 in renal tissues. Receiver operating characteristic (ROC) analysis was used to evaluate the diagnostic values of ESAT-6 and CFP-10 in IgAN associated with RT.Results: TST, serum MTB antibody, and urine MTB assessments were negative in the IgAN group. The positive rates of the TST and serum MTB antibody and urine MTB testing were higher in the RT group than in the IgAN + RT group. Among the three groups, expression levels of ESAT-6 and CFP-10 were found to be the highest in the IgAN + RT group and were found to be the lowest in the IgAN group. The ROC curves indicated that the area under curve (AUC) value of ESAT-6 protein for IgAN + RT diagnosis was 0.907 with a cut-off of 26.72 as the critical value. Detection by ESAT-6 protein levels achieved 75.0% sensitivity and 94.2% specificity. The AUC value of the CFP-10 protein for diagnosis of IgAN + RT was 0.800, with a cut-off of 25.665 as the critical value. Detection by the protein levels of CFP-10 showed 63.9% sensitivity and 84.6% specificity.Conclusions: Our study provides evidence for the potential of the proteins ESAT-6 and CFP-10 as candidate markers for the diagnosis of IgAN associated with RT.

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CFP10 and ESAT6 aptamers as effective Mycobacterial antigen diagnostic reagents

Cited by 62 publications

References 49 publications

Application of aptamers in diagnostics, drug-delivery and imaging

Application of aptamers in diagnostics, drug-delivery and imaging

Immunogenicity and protective efficacy of recombinant Bacille Calmette-Guerin strains expressing mycobacterium antigens Ag85A, CFP10, ESAT-6, GM-CSF and IL-12p70

Early secretory antigenic target 6 and culture filtrate protein 10 as diagnostic indicators in IgA nephropathy associated with renal tuberculosis

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