CFE-1, a Novel Plasmid-Encoded AmpC β-Lactamase with an<i>ampR</i>Gene Originating from<i>Citrobacter freundii</i>

Nakano, Ryuichi; Okamoto, Ryoichi; Nakano, Yumiko; Kaneko, Ken‐ichi; Okitsu, Naohiro; Hosaka, Yoshio; Inoue, Matsuhisa

doi:10.1128/aac.48.4.1151-1158.2004

Cited by 51 publications

(42 citation statements)

References 41 publications

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“…A multiplex PCR technique was used to detect plasmid-mediated ampC genes, including bla CMY , bla MOX , bla DHA , bla ACC , bla ACT , bla FOX , and bla CFE (26,27). The PCR-NheI method was used to discriminate between bla SHV-ESBL and bla SHV-non-ESBL genes (28), and nucleotide sequencing of bla SHV was performed for isolates with inconsistent results of genotyping and phenotyping.…”

Section: Methodsmentioning

confidence: 99%

Allocation of Klebsiella pneumoniae Bloodstream Isolates into Four Distinct Groups byompK36Typing in a Taiwanese University Hospital

et al. 2015

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The OmpK36 porin plays a role in carbapenem resistance and may contribute to bacterial virulence in Klebsiella pneumoniae. This study aimed to investigate the characteristics of different groups of K. pneumoniae separated by ompK36 typing. Among 226 nonduplicate K. pneumoniae bloodstream isolates collected at a Taiwanese hospital in 2011, four ompK36 types, designated types A, B, C, and D, were identified by PCR in 61, 28, 100, and 36 isolates, respectively; 1 isolate was untypeable. Statistical analysis showed significantly higher rates of antimicrobial resistance (all tested antibiotics except meropenem), extended-spectrum ␤-lactamases or DHA-1 (47.5% together), Qnr-type quinolone resistance determinants (50.8%), and IncFIIA-type plasmids (49.2%) in group A than in others. Seventeen isolates were identified as belonging to 3 international high-risk clones (4 sequence type 11 [ST11], 10 ST15, and 3 ST147 isolates); all isolates but 1 ST15 isolate were classified in group A. The significant characteristics of group C were hypermucoviscosity (62.0%) and a higher virulence gene content. This group included all serotype K1 (n ‫؍‬ 30), K2 (n ‫؍‬ 25), and K5 (n ‫؍‬ 3) isolates, 6 of 7 K57 isolates, all isolates of major clones associated with pyogenic liver abscesses (29 ST23, 11 ST65, 5 ST86, 7 ST373, and 1 ST375 isolates), and 16 (94.1%) of 17 isolates causing bacteremic liver abscesses. Twelve (42.9%) of the group B isolates were responsible for bacteremic biliary tract infections. Group D was predominant (83.3%) among 12 K20 isolates. This study suggests that most clinical K. pneumoniae isolates can be allocated into four groups with distinct characteristics based on ompK36 types. K lebsiella pneumoniae is an important human opportunistic pathogen that causes a wide variety of community-acquired and nosocomial infections (1-4). The organism is still one of the leading causes of community-acquired pneumonia (3) and has been described as the major cause of pyogenic liver abscesses (PLA) in some Asia-Pacific countries (2, 4). Serotypes K1 and K2 in K. pneumoniae have been linked to invasive infections (2-9). Using multilocus sequence typing (MLST), Brisse et al. (10) identified two virulent K1 clones of K. pneumoniae, clonal complex (CC) 23 and CC82, which were strongly associated with PLA and respiratory infection, respectively. K. pneumoniae is also an important reservoir of antibiotic resistance determinants (1). Several clones of K. pneumoniae with a high prevalence of carbapenem resistance or extended-spectrum ␤-lactamases (ESBLs) have been distributed worldwide (11-16). The epidemic drug-resistant clones, called "high-risk" clones (11), may play an important role in the dissemination of antimicrobial resistance. The major international high-risk clones identified by MLST belong to sequence type 11 (ST11), ST15, ST147,. ST11 and ST258 were included in clonal group (CG) 258 (11,14), while ST15 and ST147 were grouped in CG15 and CG147 (11, 13, 14), respectively.High-level carbapenem resistance in K. pneumoniae ma...

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Section: Methodsmentioning

confidence: 99%

Allocation of Klebsiella pneumoniae Bloodstream Isolates into Four Distinct Groups byompK36Typing in a Taiwanese University Hospital

et al. 2015

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“…Some bla genes have been revealed to associate with mobile insertion sequences such as IS26 and ISEcp1 (Saladin et al, 2002;Ford & Avison, 2004;Nakano et al, 2004). Detection of insertion sequences IS26 and ISEcp1 associated with b-lactamase genes was performed by PCR analysis (Eckert et al, 2006).…”

Section: Methodsmentioning

confidence: 99%

Regional outbreak of CTX-M-2 β-lactamase-producing Proteus mirabilis in Japan

Nakano

Abe

et al. 2012

Journal of Medical Microbiology

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Proteus mirabilis is a common cause of urinary tract infection. Wild-type P. mirabilis strains are usually susceptible to penicillins and cephalosporins, but occurrences of P. mirabilis producing extended-spectrum b-lactamases (ESBLs) have been recently reported. Here, we surveyed the prevalence of cefotaxime resistance among P. mirabilis strains at seven different hospitals in Kanagawa Prefecture, Japan, and investigated their molecular epidemiology to explain the mechanism of their spread. The prevalence of cefotaxime resistance among P. mirabilis increased annually, from 10.1 % in 1998 to 23.1 % in 2003, and increased drastically in 2004, exceeding 40 %. We collected 105 consecutive and non-duplicate cefotaxime-resistant P. mirabilis isolates (MIC 16 to .256 mg ml "1 ) from these hospitals from June 2004 to May 2005 and characterized their profile. PCR and sequence analysis revealed that all resistant strains produced exclusively CTX-M-2 b-lactamase. PFGE analysis identified 47 banding patterns with 83 % or greater similarity. These results indicated that a regional outbreak of P. mirabilis producing CTX-M-2 blactamase has occurred in Japan and suggest that the epidemic spread occurred within and across hospitals and communities by extended clonal strains. Plasmid analysis revealed that 44.8 % of plasmids harboured by bla CTX-M-2 isolates had common profiles, encoding ISEcp1, IS26 and Int1, and belonged to incompatibility group T. Spread of the resistant isolates in Japan resulted from dissemination of narrow-host-range plasmids of the IncT group encoding bla CTX-M-2 . These findings indicate the rapidly developing problem of treating the species to prevent dissemination of ESBL producers.

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“…In Korea (14,26), DHA-, CMY/MOX-, and ACT-1/MIR-1-type enzymes have also been identified, while in the United States (1,17), in addition to the types mentioned above, DHA-, ACT-1/MIR-1-, and FOX-type enzymes have been identified. To date, in Japan, MOX-1 (11), CMY-9 (9,28), , CFE-1 (19), CMY-2 (18), and DHA-1 (18) have been found in clinical isolates. Muratani et al (18) reported PABL producers among cephem-resistant K. pneumoniae isolates, but this report did not indicate the rate of occurrence of PABLs.…”

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confidence: 99%

Laboratory Surveillance for Prospective Plasmid-Mediated AmpC β-Lactamases in the Kinki Region of Japan

Yamasaki

Komatsu²,

Abe

et al. 2010

J Clin Microbiol

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Extended-spectrum ␤-lactamases, plasmid-mediated AmpC ␤-lactamases (PABLs), and plasmid-mediated metallo-␤-lactamases confer resistance to many ␤-lactams. In Japan, although several reports exist on the prevalence of extended-spectrum ␤-lactamases and metallo-␤-lactamases, the prevalence and characteristics of PABLs remain unknown. To investigate the production of PABLs, a total of 22,869 strains of 4 enterobacterial species, Escherichia coli, Klebsiella pneumoniae, Klebsiella oxytoca, and Proteus mirabilis, were collected during six 6-month periods from 17 clinical laboratories in the Kinki region of Japan. PABLs were detected in 29 (0.13%) of 22,869 isolates by the 3-dimensional test, PCR analysis, and DNA sequencing analysis. PABL-positive isolates were detected among isolates from 13 laboratories. Seventeen of 13,995 (0.12%) E. coli isolates, 8 of 5,970 (0.13%) K. pneumoniae isolates, 3 of 1,722 (0.17%) K. oxytoca isolates, and 1 of 1,182 (0.08%) P. mirabilis isolates were positive for PABLs. Of these 29 PABL-positive strains, 20 (69.0%), 6 (20.7%), 2 (6.9%), and 1 (3.4%) carried the genes for CMY-2, DHA-1, CMY-8, and MOX-1 PABLs, respectively. Pattern analysis of randomly amplified polymorphic DNA and pulsed-field gel electrophoretic analysis revealed that the prevalence of CMY-2-producing E. coli strains was not due to epidemic strains and that 3 DHA-1-producing K. pneumoniae strains were identical, suggesting their clonal relatedness. In conclusion, the DHA-1 PABLs were predominantly present in K. pneumoniae strains, but CMY-2 PABLs were predominantly present in E. coli strains. The present findings will provide significant information to assist in preventing the emergence and further spread of PABL-producing bacteria.␤-Lactamase production is the most important factor for ␤-lactam resistance in Gram-negative rods (16). Plasmid-mediated ␤-lactamases, such as extended-spectrum ␤-lactamases (ESBLs), plasmid-mediated AmpC ␤-lactamases (PABLs), and plasmid-mediated metallo-␤-lactamases (MBLs), hydrolyze broad-spectrum ␤-lactams. Detection of these plasmidmediated ␤-lactamase-producing isolates is important for epidemiological studies and hospital infection control, because plasmid-mediated genes can spread to other organisms.The Study of Bacterial Resistance in the Kinki Region of Japan (SBRK) Antimicrobial Surveillance Program was established in 1997 to monitor the predominant pathogens and antimicrobial resistance patterns of nosocomial and communityacquired infections via a broad network of clinical laboratories differing in geographic location and size. Our previously reported survey data from the Kinki region of Japan revealed the prevalence of ESBLs and plasmid-mediated MBLs (21, 30); however, the epidemiology of PABLs remains unknown. For this reason, a laboratory-based surveillance study was conducted to determine the presence and prevalence of PABLs among members of the family Enterobacteriaceae.

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CFE-1, a Novel Plasmid-Encoded AmpC β-Lactamase with anampRGene Originating fromCitrobacter freundii

Cited by 51 publications

References 41 publications

Allocation of Klebsiella pneumoniae Bloodstream Isolates into Four Distinct Groups byompK36Typing in a Taiwanese University Hospital

Allocation of Klebsiella pneumoniae Bloodstream Isolates into Four Distinct Groups byompK36Typing in a Taiwanese University Hospital

Regional outbreak of CTX-M-2 β-lactamase-producing Proteus mirabilis in Japan

Laboratory Surveillance for Prospective Plasmid-Mediated AmpC β-Lactamases in the Kinki Region of Japan

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