The paper presents the research results of the injector construction with the modified injection nozzle. The injector is designed for a prototype opposed-piston aircraft diesel engine. The measurements were based on the Mie scattering technique. The conditions of the experiment corresponded to maximum loads similar to those occurring at the start. The measuring point was selected in line with the analysis of engine operating conditions: combustion chamber pressure at the moment of fuel delivery (6 MPa) and fuel pressure in the injection rail (140 MPa). The analysis focused on the average spray range and distribution, taking into account the differences between holes in the nozzle. As a result of the conducted research, the fuel spray range was defined with the determined parameters of injection. The fuel spray ranges inside the constant volume chamber at specific injection pressures and in the chamber were examined, and the obtained results were used to verify and optimize the combustion process in the designed opposed-piston two-stroke engine.