“…Moreover, the in vitro reaction format of CFPS systems allows for full automation, miniaturization ( Ayoubi-Joshaghani et al, 2020 ), and working with large sample numbers ( Zhu et al, 2015 ). This advantage has been utilized in large-scale screening experiments ( Khnouf et al, 2010 ; Kim et al, 2015 ), searches for malaria vaccine candidates ( Kanoi et al, 2017 ; Morita et al, 2017 ; Kanoi et al, 2020 ), identifying interactions between E3 ligases and their substrates ( Takahashi et al, 2016 ), building a protein array holding human Deubiquitinating Enzymes (DUBs) ( Takahashi et al, 2020 ), or the development of protein array platforms ( Romanov et al, 2014 ; Zarate and Galbraith, 2014 ; Morishita et al, 2019 ). Other promising developments make use of the stability of the reagents, where the extracts and buffers can be lyophilized for long-term storage at room temperature ( Smith et al, 2014 ).…”