CESTA, a positive regulator of brassinosteroid biosynthesis

Poppenberger, Brigitte; Rozhon, Wilfried; Khan, Mamoona; Husar, Sigrid; Adam, Gerhard; Luschnig, Christian; Fujioka, Shozo; Sieberer, Tobias

doi:10.1038/emboj.2011.35

Cited by 110 publications

(108 citation statements)

References 64 publications

(107 reference statements)

Supporting

Mentioning

104

Contrasting

Order By: Relevance

“…In Arabidopsis, the GSK3-like kinase BIN2 was reported to target both BES1/BZR1 and ARF2 to function in either BR responses or BR-mediated auxin responses Yin et al, 2002;Vert et al, 2008). In addition, it was found AIF1 and CESTA, two basic helix-loop-helix proteins involved in BR responses, can also be phosphorylated by BIN2 in vitro Poppenberger et al, 2011). Very recently, YDA, an MAPKKK, was reported to function as a substrate of BIN2 to mediate BR regulation of stomatal development (Kim et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

DWARF AND LOW-TILLERING Acts as a Direct Downstream Target of a GSK3/SHAGGY-Like Kinase to Mediate Brassinosteroid Responses in Rice

et al. 2012

View full text Add to dashboard Cite

In Arabidopsis thaliana, the GSK3/SHAGGY-like kinase BRASSINOSTEROID-INSENSITIVE2 (BIN2) plays a critical role in the brassinosteroid (BR) signaling pathway by negatively regulating the activities of bri1-EMS-SUPPRESSOR1/BRASSINAZOLE-RESISTANT1 family transcription factors that regulate the expression of downstream BR-responsive genes. In this study, we analyzed the function of a rice (Oryza sativa) GSK3/SHAGGY-like kinase (GSK2), which is one of the orthologs of BIN2. Overexpression of GSK2 (Go) led to plants with typical BR loss-of-function phenotypes, and suppression of GSK2 resulted in enhanced BR signaling phenotypes. DWARF AND LOW-TILLERING (DLT) is a positive regulator that mediates several BR responses in rice. Suppression of DLT can enhance the phenotypes of BR receptor mutant d61-1, and overexpression of DLT obviously suppressed the BR loss-of-function phenotypes of both d61-1 and Go, suggesting that DLT functions downstream of GSK2 to modulate BR responses. Indeed, GSK2 can interact with DLT and phosphorylate DLT. Moreover, brassinolide treatment can induce the dephosphorylation of DLT, leading to the accumulation of dephosphorylated DLT protein. In GSK2 transgenic plants, the DLT phosphorylation level is dictated by the GSK2 level. These results demonstrate that DLT is a GSK2 substrate, further reinforcing that the BIN2/GSK2 kinase has multiple substrates that carry out various BR responses.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Several atypical basic helix-loop-helix proteins were found to be involved in BR signaling either positively or negatively Zhang et al, 2009). At least three other transcription factors have been found to regulate BR biosynthesis or signaling components Je et al, 2010;Poppenberger et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

DWARF AND LOW-TILLERING Acts as a Direct Downstream Target of a GSK3/SHAGGY-Like Kinase to Mediate Brassinosteroid Responses in Rice

et al. 2012

View full text Add to dashboard Cite

show abstract

“…Two hours after treatment ;0.6 g plant material was harvested per sample. Cross-linking and chromatin preparation were performed as described previously (Poppenberger et al, 2011). For immunoprecipitation, anti-GFP VHH agarose beads (Chromotek) and agarose beads as control were used.…”

Section: Chip and Emsasmentioning

confidence: 99%

“…The DNA fragments used in EMSA as probes were PCR amplified and purified using the E.N.Z.A Gel Extraction Kit (OMEGA Bio-tek), and 1 ng DNA was used for adenosine 59-[g-33 P] triphosphate (American Radiolabeled Chemicals) labeling by PNK (Thermo Scientific) and again purified. The EMSA experiments were performed as described previously (Poppenberger et al, 2011).…”

Section: Chip and Emsasmentioning

confidence: 99%

Brassinosteroids Are Master Regulators of Gibberellin Biosynthesis in Arabidopsis

et al. 2015

Self Cite

View full text Add to dashboard Cite

“…24 Complementary patterns were observed, particularly at specific locations of the reproductive tract through which pollen tubes grow to reach the ovules. 11,12 In other plant tissues, auxins and cytokinins are closely related and together guide the development of different organs. [13][14][15][16][17] In the gynoecium, cytokinins promote the growth of the placenta and ovules.…”

Section: Hormones Talkingmentioning

confidence: 99%

Hormones talking

Marsch-Martínez

Reyes-Olalde

Ramos-Cruz

et al. 2012

Plant Signaling & Behavior

View full text Add to dashboard Cite

Addendum to: Marsch-Martínez N, Ramos-Cruz D, Irepan Reyes-Olalde J, Lozano-Sotomayor P, Zúñiga-Mayo VM, de Folter S. The role of cytokinin during Arabidopsis gynoecia and fruit morphogenesis and patterning. Plant J 2012; 72:222-234 T he proper development of fruits is important for the sexual reproduction and propagation of many plant species. The fruit of Arabidopsis derives from the fertilized gynoecium, which initiates at the center of the flower and obtains its final shape, size, and functional tissues through progressive stages of development. Hormones, specially auxins, play important roles in gynoecium and fruit patterning. Cytokinins, which act as counterparts to auxins in other plant tissues, have been studied more in the context of ovule formation and parthenocarpy. We recently studied the role of cytokinins in gynoecium and fruit patterning and found that they have more than one role during gynoecium and fruit patterning. We also compared the cytokinin response localization to the auxin response localization in these organs, and studied the effects of spraying cytokinins in young flowers of an auxin response line. In this addendum, we discuss further the implications of the observed results in the knowledge about the relationship between cytokinins and auxins at the gynoecium.Fruits are plant organs that nurture, protect, and facilitate seed dispersal and are therefore very important for successful plant propagation through sexual reproduction in many species. Most fruits originate from the female reproductive organ, the gynoecium, after ovules inside become fertilized by the pollen. From top to bottom, the Arabidopsis gynoecium is formed by a stigma, style, ovary,

show abstract

CESTA, a positive regulator of brassinosteroid biosynthesis

Cited by 110 publications

References 64 publications

DWARF AND LOW-TILLERING Acts as a Direct Downstream Target of a GSK3/SHAGGY-Like Kinase to Mediate Brassinosteroid Responses in Rice

DWARF AND LOW-TILLERING Acts as a Direct Downstream Target of a GSK3/SHAGGY-Like Kinase to Mediate Brassinosteroid Responses in Rice

Brassinosteroids Are Master Regulators of Gibberellin Biosynthesis in Arabidopsis

Hormones talking

Contact Info

Product

Resources

About