Cervicovaginal microbiota isolated from healthy women exhibit probiotic properties and antimicrobial activity against pathogens isolated from cervical cancer patients

Kamble, Ashwini; Naik, Shilpa; Talathi, Manju; Jadhav, Deepali; Pingale, Samradni; Kaul-Ghanekar, Ruchika

doi:10.1007/s00203-022-03103-5

Cited by 6 publications

(9 citation statements)

References 98 publications

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“…Government Medical College and Sassoon General Hospitals (BJGMCSGH), Pune (Ref No. BJGMC/IEC/Pharmac/ND-Dept 0,119,007-007) as mentioned in our previous study [4].…”

Section: Acknowledgementmentioning

confidence: 69%

“…Government Medical College and Sassoon General Hospitals (BJGMCSGH), Pune (Ref No. BJGMC/ IEC/Pharmac/ND-Dept 0,119,007-007) as mentioned in our previous study [4]. All the participants provided informed consent forms and were categorized as described earlier [4].…”

Section: Selection Of Subjectsmentioning

confidence: 99%

“…The study was performed as per guidelines of 1964 Helsinki declaration for collecting specimen from human patients [4]. All the procedures involving human patients were approved by Ethics Committees of Bharati Vidyapeeth (Deemed to be) University Medical College (BVMC) (Ref: BVDU/MC/57), and B. J.…”

Section: Acknowledgementmentioning

confidence: 99%

“…The vaginal microbiota of healthy women is mostly dominated by lactic acid bacteria (LAB), such as Lactobacillus that produce various short-chain fatty acids and organic acids [3]. We recently reported dominance of LAB in healthy Indian women [4] and their antimicrobial activity against vaginal pathogens, isolated from ICC patients. The cell-free supernatant (CFS) of probiotic LAB revealed the presence of metabolites with reported antimicrobial activity.…”

Section: Introductionmentioning

confidence: 99%

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Exploring the diversity of vaginal microbiota between healthy women and cervical cancer patients in India

Kamble,

Naik,

Talathi

et al. 2024

Journal of Medical Microbiology

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Introduction. Cervicovaginal diversity has been reported as a predictive biomarker for cervical cancer risk. We recently reported the bio-therapeutic potential of vaginal probiotics from healthy Indian women against vaginal pathogens, isolated from the invasive cervical cancer (ICC) patients. Gap Statement. The cervicovaginal microflora from cervical cancer patients has not yet been reported from Indian population. Aim. The present study aimed at comparing the cervicovaginal microbiome between healthy controls (HC) and ICC patients from the Indian population. Methodology. In total, 30 vaginal swabs (15 from HC and 15 from ICC) were subjected to 16S rRNA gene sequencing. Alpha diversity was evaluated by Shannon and Chao1 index; and beta diversity by principle coordinate analysis (PCoA) of weighted and unweighted UniFrac distances. The relative abundance of the microbial taxa was done according to linear discriminant analysis effect size (LEfSe). Results. Predominance of Staphylococcus spp. in ICC and Lactobacillus gasseri in HC groups was observed. Alpha-diversity was found to be higher in ICC as compared to HC but was statistically non-significant. LEfSe analysis revealed Bacteroides fragilis and Escherichia coli as the marker genera in ICC with a marked decrease in Lactobacillus sp. Contrarily, in HC, L. gasseri, L. iners and L. fermentum were found to be abundant. Conclusion. Differences in the vaginal microbiome between healthy and ICC women could help in the early prediction of cervical cancer risk and thus in designing prevention strategies.

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“…Government Medical College and Sassoon General Hospitals (BJGMCSGH), Pune (Ref No. BJGMC/IEC/Pharmac/ND-Dept 0,119,007-007) as mentioned in our previous study [4].…”

Section: Acknowledgementmentioning

confidence: 69%

Section: Selection Of Subjectsmentioning

confidence: 99%

Section: Acknowledgementmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Exploring the diversity of vaginal microbiota between healthy women and cervical cancer patients in India

Kamble,

Naik,

Talathi

et al. 2024

Journal of Medical Microbiology

Self Cite

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show abstract

“…Some authors have connected the expression of proinflammatory and chemotactic cytokines related to HPV-induced carcinogenesis with an increased presence of Sneathia or Gardnerella in the vaginal microenvironment ( 86 – 90 ). Furthermore, even though a certain level of inflammation has been described as potentially beneficial to decrease HPV dissemination, several studies have shown specific inflammation markers as related to the progression to a carcinogenic status that could be used as clinical markers to prevent high-grade squamous intraepithelial lesions ( 46 , 86 , 90 – 95 ) and specific metabolic profiles ( 96 – 98 ). However, most studies in the field are cross-sectional, so it is hard to assess whether the microbiota influences HPV infection or vice versa.…”

Section: Influence Of the Microbiota On Hiv And Hpv Infectionsmentioning

confidence: 99%

The microbiota as a modulator of mucosal inflammation and HIV/HPV pathogenesis: From association to causation

2023

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Although the microbiota has largely been associated with the pathogenesis of viral infections, most studies using omics techniques are correlational and hypothesis-generating. The mechanisms affecting the immune responses to viral infections are still being fully understood. Here we focus on the two most important sexually transmitted persistent viruses, HPV and HIV. Sophisticated omics techniques are boosting our ability to understand microbiota-pathogen-host interactions from a functional perspective by surveying the host and bacterial protein and metabolite production using systems biology approaches. However, while these strategies have allowed describing interaction networks to identify potential novel microbiota-associated biomarkers or therapeutic targets to prevent or treat infectious diseases, the analyses are typically based on highly dimensional datasets —thousands of features in small cohorts of patients—. As a result, we are far from getting to their clinical use. Here we provide a broad overview of how the microbiota influences the immune responses to HIV and HPV disease. Furthermore, we highlight experimental approaches to understand better the microbiota-host-virus interactions that might increase our potential to identify biomarkers and therapeutic agents with clinical applications.

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Cervicovaginal Bacillus velezensis Isolate: A Potential Probiotic and an Antagonist Against Candida and Staphylococcus

Sahal,

Donmez,

Beksac

2023

Curr Microbiol

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Cervicovaginal microbiota isolated from healthy women exhibit probiotic properties and antimicrobial activity against pathogens isolated from cervical cancer patients

Cited by 6 publications

References 98 publications

Exploring the diversity of vaginal microbiota between healthy women and cervical cancer patients in India

Exploring the diversity of vaginal microbiota between healthy women and cervical cancer patients in India

The microbiota as a modulator of mucosal inflammation and HIV/HPV pathogenesis: From association to causation

Cervicovaginal Bacillus velezensis Isolate: A Potential Probiotic and an Antagonist Against Candida and Staphylococcus

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