Cervicovaginal Lamina Propria Lymphocytes: Phenotypic Characterization and Their Importance in Cytotoxic T-Lymphocyte Responses to Simian Immunodeficiency Virus SIV<sub>mac251</sub>

Stevceva, Liljana; Kelsall, Brian L.; Nacsa, János; Moniuszko, Marcin; Hel, Zdeněk; Tryniszewska, Elżbieta; Franchini, Genoveffa

doi:10.1128/jvi.76.1.9-18.2002

Cited by 46 publications

(37 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The increased expression of adhesion molecules and chemokine receptors on HIV-specific CD8 ϩ T cells in CSF during HIV infection also suggests that the trafficking of antigen-specific T cells to the brain may be due to a combination of the systemic activation of T cells and response to intrathecal inflammation (69). Our finding may not be unique to the brain, since in SIV-infected macaques high frequencies of virus-specific T cells have been demonstrated in the gastrointestinal tract, urogenital tissue, spleen, bone marrow, and liver compared to what is seen for peripheral blood (32,46,68,73). Recent data from a rodent model suggest that the expression of viral antigens in the brain may upregulate endothelial cell major histocompatibility complex class I expression, which in turn favors CD8 ϩ T-cell migration into the brain (27).…”

Section: Discussionmentioning

confidence: 61%

Enhancement of Human Immunodeficiency Virus (HIV)-Specific CD8⁺T Cells in Cerebrospinal Fluid Compared to Those in Blood among Antiretroviral Therapy-Naïve HIV-Positive Subjects

Sadagopal

Lorey

Barnett

et al. 2008

J Virol

View full text Add to dashboard Cite

During untreated human immunodeficiency virus type 1 (HIV-1) infection, virus-specific CD8؉ T cells partially control HIV replication in peripheral lymphoid tissues, but host mechanisms of HIV control in the central nervous system (CNS) are incompletely understood. We characterized HIV-specific CD8 ؉ T cells in cerebrospinal fluid (CSF) and peripheral blood among seven HIV-positive antiretroviral therapy-naïve subjects. All had grossly normal brain magnetic resonance imaging and spectroscopy and normal neuropsychometric testing. Frequencies of epitope-specific CD8 ؉ T cells by direct tetramer staining were on average 2.4-fold higher in CSF than in blood (P ‫؍‬ 0.0004), while HIV RNA concentrations were lower. Cells from CSF were readily expanded ex vivo and responded to a broader range of HIV-specific human leukocyte antigen class I restricted optimal peptides than did expanded cells from blood. HIV-specific CD8 ؉ T cells, in contrast to total CD8 ؉ T cells, in CSF and blood were at comparable maturation states, as assessed by CD45RO and CCR7 staining. The strong relationship between higher T-cell frequencies and lower levels of viral antigen in CSF could be the result of increased migration to and/or preferential expansion of HIV-specific T cells within the CNS. This suggests an important role for HIV-specific CD8 ؉ T cells in control of intrathecal viral replication.

show abstract

Section: Discussionmentioning

confidence: 61%

Enhancement of Human Immunodeficiency Virus (HIV)-Specific CD8⁺T Cells in Cerebrospinal Fluid Compared to Those in Blood among Antiretroviral Therapy-Naïve HIV-Positive Subjects

Sadagopal

Lorey

Barnett

et al. 2008

J Virol

View full text Add to dashboard Cite

show abstract

“…In previous studies, no determination was made about whether the observed decrease in CD4 + T cells in the FRT was due to a reduction in the number of CD4 + T cells and/or an increase in the number of CD8 + T cells (33,49,50). To address this important question, we performed a longitudinal analysis of CD4 + and CD8 + T cell numbers in CVS following HIV infection.…”

Section: Reconstitution Of the Frt Of Blt Mice With Human Cd4mentioning

confidence: 99%

ART influences HIV persistence in the female reproductive tract and cervicovaginal secretions

Olesen

Swanson

Kovářová

et al. 2016

Journal of Clinical Investigation

View full text Add to dashboard Cite

“…(iii) The emergence of escape variants indicates selective pressure exerted by the CD8 ϩ T lymphocytes (3,22). We specifically investigated the Mamu-A*01-restricted immunodominant epitopes, Gag 181-189 CM9 and Tat [28][29][30][31][32][33][34][35] SL8, because the response to these two epitopes accounts for the majority of detectable CD8…”

Section: ؉ T Lymphocytes In Gut-associated Lymphatic Tissue (Galt) Wmentioning

confidence: 99%

CD8⁺T-Lymphocyte Response to Major Immunodominant Epitopes after Vaginal Exposure to Simian Immunodeficiency Virus: Too Late and Too Little

Reynolds

Rakasz²,

Skinner

et al. 2005

J Virol

152

154

View full text Add to dashboard Cite

In the acute stage of infection following sexual transmission of human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV), virus-specific CD8؉ T-lymphocyte responses partially control but do not eradicate infection from the lymphatic tissues (LTs) or prevent the particularly massive depletion of CD4 ؉ T lymphocytes in gut-associated lymphatic tissue (GALT). We explored hypothetical explanations for this failure to clear infection and prevent CD4 ؉ T-lymphocyte loss in the SIV/rhesus macaque model of intravaginal transmission. We examined the relationship between the timing and magnitude of the CD8 ؉ T-lymphocyte response to immunodominant SIV epitopes and viral replication, and we show first that the failure to contain infection is not because the female reproductive tract is a poor inductive site. We documented robust responses in cervicovaginal tissues and uterus, but only several days after the peak of virus production. Second, while we also documented a modest response in the draining genital and peripheral lymph nodes, the response at these sites also lagged behind peak virus production in these LT compartments. Third, we found that the response in GALT was surprisingly low or undetectable, possibly contributing to the severe and sustained depletion of CD4 ؉ T lymphocytes in the GALT. Thus, the virus-specific CD8 ؉ T-lymphocyte response is "too late and too little" to clear infection and prevent CD4 ؉ T-lymphocyte loss. However, the robust response in female reproductive tissues may be an encouraging sign that vaccines that rapidly induce high-frequency CD8 ؉

show abstract

Cervicovaginal Lamina Propria Lymphocytes: Phenotypic Characterization and Their Importance in Cytotoxic T-Lymphocyte Responses to Simian Immunodeficiency Virus SIV_mac251

Abstract: Most human immunodeficiency virus (HIV

Cited by 46 publications

References 62 publications

Enhancement of Human Immunodeficiency Virus (HIV)-Specific CD8⁺T Cells in Cerebrospinal Fluid Compared to Those in Blood among Antiretroviral Therapy-Naïve HIV-Positive Subjects

Enhancement of Human Immunodeficiency Virus (HIV)-Specific CD8⁺T Cells in Cerebrospinal Fluid Compared to Those in Blood among Antiretroviral Therapy-Naïve HIV-Positive Subjects

ART influences HIV persistence in the female reproductive tract and cervicovaginal secretions

CD8⁺T-Lymphocyte Response to Major Immunodominant Epitopes after Vaginal Exposure to Simian Immunodeficiency Virus: Too Late and Too Little

Contact Info

Product

Resources

About

Cervicovaginal Lamina Propria Lymphocytes: Phenotypic Characterization and Their Importance in Cytotoxic T-Lymphocyte Responses to Simian Immunodeficiency Virus SIVmac251

Abstract: Most human immunodeficiency virus (HIV

Cited by 46 publications

References 62 publications

Enhancement of Human Immunodeficiency Virus (HIV)-Specific CD8+T Cells in Cerebrospinal Fluid Compared to Those in Blood among Antiretroviral Therapy-Naïve HIV-Positive Subjects

Enhancement of Human Immunodeficiency Virus (HIV)-Specific CD8+T Cells in Cerebrospinal Fluid Compared to Those in Blood among Antiretroviral Therapy-Naïve HIV-Positive Subjects

ART influences HIV persistence in the female reproductive tract and cervicovaginal secretions

CD8+T-Lymphocyte Response to Major Immunodominant Epitopes after Vaginal Exposure to Simian Immunodeficiency Virus: Too Late and Too Little

Contact Info

Product

Resources

About

Cervicovaginal Lamina Propria Lymphocytes: Phenotypic Characterization and Their Importance in Cytotoxic T-Lymphocyte Responses to Simian Immunodeficiency Virus SIV_mac251

Enhancement of Human Immunodeficiency Virus (HIV)-Specific CD8⁺T Cells in Cerebrospinal Fluid Compared to Those in Blood among Antiretroviral Therapy-Naïve HIV-Positive Subjects

Enhancement of Human Immunodeficiency Virus (HIV)-Specific CD8⁺T Cells in Cerebrospinal Fluid Compared to Those in Blood among Antiretroviral Therapy-Naïve HIV-Positive Subjects

CD8⁺T-Lymphocyte Response to Major Immunodominant Epitopes after Vaginal Exposure to Simian Immunodeficiency Virus: Too Late and Too Little