Cervical cytology biobanking: quality of DNA from archival cervical Pap-stained smears

Boulet, Gaëlle; Horvath, Caroline A.J.; Berghmans, Sarah; Moeneclaey, Liliane M.; Duys, Inge; Arbyn, Marc; Depuydt, Christophe; Vereecken, Annie; Sahebali, Shaira; Bogers, Johannes

doi:10.1136/jcp.2007.052688

Cited by 25 publications

(19 citation statements)

References 37 publications

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“…Persistence of a type over time, sensitive type-specific in situ hybridization, or use of mRNA based tests could clarify this question (51,52). Storage of all residual cellular material, extraction and freezing of DNA, and several feasibility studies involving testing of archived material further enhance the possibility to disentangle the natural history of HPV infections at the type-specific level (53)(54)(55).…”

Section: Discussionmentioning

confidence: 99%

Prevaccination Distribution of Human Papillomavirus Types in Women Attending at Cervical Cancer Screening in Belgium

Arbyn

Benoy²,

Simoens

et al. 2009

Cancer Epidemiology, Biomarkers &Amp; Prevention

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Introduction: Before the introduction of vaccination against human papillomaviruses (HPV) as a new strategy of combating cervical cancer, it is required to describe the baseline prevalence of HPV infection as well as the distribution of the different HPV types in the population and among women with cervical lesions. Materials and Methods: Approximately 10,000 liquid cervical cell samples from women, resident of Flanders (North Belgium) and participating in cervical cancer screening, were assessed cytologically and virologically with a multiplex real-time PCR using primers targeting the E6/E7 genes of 16 HPV types. Correlations of HPV infection with age, geographic area, and occurrence of cytologic lesions were assessed. Results: The prevalence of cytologic abnormalities was atypical squamous cells of undetermined significance (ASC-US), 1.6%; atypical glandular cells (AGC), 0.2%; low-grade squamous intraepithelial lesion (LSIL), 2.6%; atypical squamous cells, HSIL cannot be excluded (ASC-H), 0.3%; and high-grade squamous intraepithelial lesion (HSIL), 1.2%. The frequency of high-risk HPV infections was 11% in women without cytologic abnormalities, 77% in ASC-US, 32% in AGC, 85% in LSIL, and 93% in ASC-H and HSIL. The prevalence of high-risk HPV infection was highest in

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Section: Discussionmentioning

confidence: 99%

Prevaccination Distribution of Human Papillomavirus Types in Women Attending at Cervical Cancer Screening in Belgium

Arbyn

Benoy²,

Simoens

et al. 2009

Cancer Epidemiology, Biomarkers &Amp; Prevention

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“…However, for nucleic acid extracts from fixed and stained conventional smears, spectrophotometric approaches are often considered insufficient because of protein cross-linking and the presence of compounds that interfere with light absorption (24).…”

Section: Spectrophotometrymentioning

confidence: 99%

Cervical Cytology Biobanking in Europe

Arbyn

Veen²,

Andersson

et al. 2010

Int J Biol Markers

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Int J Biol Markers ( 2010; 3 ): 117-125 25 - 117Original article offers a unique tool for etiological studies, studies on the natural history and molecular pathways of carcinogenesis, evaluation of screening and vaccination effectiveness, and the evaluation of early biomarkers (viral DNA, RNA or proteins, or immunological, genetic or other biological host factors) usable in cancer screening or triage of screen positives. Even when, in the future, detection of nucleic acids of human papillomaviruses would become the standard screening methodology, collection of cellular material will be needed to perform HPV tests. Removal of coverslips from archived conventional Pap smears, extraction of DNA from scraped cells or from microdissected dyskaryotic cells, DNA amplification of human or viral genomic sequences and HPV genotyping have been demonstrated to be feasible in previous studies (2-6). Liquid-based cytology (LBC) is a newer method of preparing cervical cytology samples. Collected cells are transferred in a fluid fixative and evenly dispersed across slides (7). Importantly, the leftover sample contains large amounts of fixed cells with well-preserved morphology, INTRODUCTIONEvery year, tens of millions of cervical cell samples are obtained within the European Union. In countries with well organized cervical cancer screening, the 10-year cumulative attendance rate is well above 90% of all women belonging to the target age range. The average number of smears planned over a lifetime varies among member states: from 7 in Finland and the Netherlands to more than 50 in Germany, Austria and Luxembourg (1). Cervical cell samples are usually stored for 5 to 10 years for medicolegal or quality-control reasons. Certain European laboratories have archived series of Pap smears over several decades. This makes archival cervical samples a valuable resource for research where genetic susceptibility, exposure to external etiological factors as well as outcome (cervical and other malignancies) can be documented through the link with cancer registries.A cervical cytology biobank (CCB) linked to a screening registry, a cancer registry and a HPV vaccination registry

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“…(Goldie et al,2005) Liquid-based cytology has logistical and operational advantages (interpretation at higher speed, lower rate of unsatisfactory smears and possibility of ancillary molecular testing using remnant fluid), but is more expensive and is neither more sensitive nor more specific than conventional cytology with respect to detection of histologically confirmed high-grade CIN. (Arbyn et al, 2008) We must continue to recognise both the strengths and limitations of cytology for cervical cancer screening. In populations vaccinated against HPV-16/18 we should anticipate that the positive predictive value (PPV) of cervical screening will be reduced because there will be fewer high-grade lesions amongwomen with cytological abnormalities.…”

Section: Cytological Screeningmentioning

confidence: 99%

Human Papillomavirus and Related Diseases - From Bench to Bedside - A Clinical Perspective

Broeck¹

2012

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Cervical cytology biobanking: quality of DNA from archival cervical Pap-stained smears

Abstract: The study indicates that commercial DNA isolation techniques are suitable for PCR amplification of DNA isolated from archival smears, yielding amplicons up to 400 base pairs. Proteinase K digestion is not suitable to obtain amplifiable DNA from fixed and stained Pap-stained smears.

Cited by 25 publications

References 37 publications

Prevaccination Distribution of Human Papillomavirus Types in Women Attending at Cervical Cancer Screening in Belgium

Prevaccination Distribution of Human Papillomavirus Types in Women Attending at Cervical Cancer Screening in Belgium

Cervical Cytology Biobanking in Europe

Human Papillomavirus and Related Diseases - From Bench to Bedside - A Clinical Perspective

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