Cervical cancer screening by molecular Pap‐transformation of gynecologic cytology

Abstract

Background Cervical cancer is one of the common cancers in women accounting for 7.9% of all cancers. In India it is the second commonest cancer in women. The immortality of the cancer cell and the relatively long time frame from acquisition of infection to development of cervical cancer was established. As major advancements like LBC, HPV testing were introduced in the recent years, screening has taken a new avatar, the Molecular pap!! The objectives of this study were: To compare gynecologic cytology and abnormal results with respect to conventional and LBC. To study the role of HPV cotesting and ancillary tests performed, that is, HPV CISH, and p16ink4a by IHC. Methods About 71 924 Conventional and LBC samples were included from August 2009 to December 2017. Cases for HPV testing along the conventional smears were 1539. HPV can be tested from the same LBC vial as the sample remains stable at room temperature for 6 weeks. HPV DNA PCR was carried out in our laboratory for High and Low risk genotypes. Cytology findings were also correlated with histology. Results Detection rate of SILs in LBC samples were higher (2.20%). The commonest abnormality was LSIL in LBC and ASCUS in conventional smears. Commonest HR HPV and LR HPV detected was 1 61 856 and 61 142. Conclusion LBC with HPV cotesting improves sensitivity and specificity and reduces ambiguous results; allows better compliance, as a negative result of both tests allows patients to get screening every 5 years, thereby increasing screening intervals, important in a resource limited situation.

Comparative Analysis of ThinPrep and CellSolutions Liquid-Based Cervical Cytology along with Human Papillomavirus DNA Testing: A Study of 412 Cases

“…HPV 16 was the commonest genotype (18.75%), followed by HPV 18 (15.62%), similar to a study from India with a detection rate of 11.9%. [28] The overall HPV positivity in ThinPrep was 11.11%, the detection rate of high-risk HPV genotype was 82.4% and low-risk HPV genotype was 17.6%. Similar to this study, the most commonly observed genotypes are HPV 16, 56, 18, and 42.…”

Prevalence of abnormal pap smears in the western region of Saudi Arabia from 2010 to 2022

“…LBC also allows for the molecular testing of HPV from the same vial, as long as approximately 2 mL of sample is sent for molecular biology first (to avoid contamination), and then routine LBC preparation is carried out. [ 13 , 14 ] For laboratories accredited by CAP, the cytopathology checklist provides benchmarking data for the acceptable reporting-percentile rate (RPR) for each category and ASC/SIL ratio for each preparation type. [ 9 ] Our data, percentages and ASC/SIL ratios, as shown in [ Table 1 ], were within the 5–95 th RPR; due to copyright, we cannot share the CAP’s RPR in our study.…”

Identification and performance evaluation of housekeeping genes for microRNA expression normalization by reverse transcription‑quantitative PCR using liquid‑based cervical cytology samples

“…In another study, Kottaridi et al (83) designed a panel of seven overexpressed miRNAs for use in histologically confirmed LBC malignant endometrial samples to discriminate between non-malignant and malignant specimens and to identify any samples with inadequate RNA. There are many studies that have reported promising molecular approaches to LBC samples for clinical laboratories (84–86). None of the studies to date have focused on evaluating miRNAs in LBC cervical samples, which could be considered an important minimally invasive approach for cancer detection by miRNA expression data.…”