Cervical cancer metabolism: Major reprogramming of metabolic pathways and cellular energy production

Rachel, K. Vijaya; Sivaraj, Nagarjuna

doi:10.1016/b978-0-12-822009-2.00012-1

Cited by 1 publication

(2 citation statements)

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“…Cervical cancer cells have reprogramed cellular metabolic pathways, such as enhanced aerobic glycolysis (Warburg effect), glutaminolytic flux, and pentose phosphate pathway (PPP) stimulation (Vijaya Rachel & Sivaraj, 2021). One recent study noticed that HPV16 E6/E7 proteins could enhance the expression of hexokinase‐ll (HK‐II) (Zeng et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

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HPV16 E6/E7 stabilize PGK1 protein by reducing its poly‐ubiquitination in cervical cancer

Liu

Song

Yao

et al. 2021

Cell Biology International

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This study aimed to explore the expression profile, prognostic value, regulatory effect, and the underlying mechanism of dysregulation of phosphoglycerate kinase 1 (PGK1) in high‐risk human papillomavirus (HPV)‐positive cervical epithelial squamous cell carcinoma (CESC). Bioinformatic analysis was performed using the CESC subset of The Cancer Genome Atlas (TCGA)‐Cervical Cancer (CESC) and normal cervix in The Genotype‐Tissue Expression (GTEx) project. HPV‐16 positive CaSki and SiHa cells were used as in vitro cell models. Results showed that compared to the normal cervix, CESC tissues had significantly higher expression of PGK1. CESC patients with the higher 50% expression of PGK1 had substantially shorter disease‐specific survival (DSS), and progression‐free survival (PFS) compared to the cases with the lower 50% expression of PGK1. PGK1 knockdown impaired, but PGK1 overexpression enhanced the proliferation, colony formation, aerobic glycolytic activities (lactate production, intracellular ATP levels, glucose uptake, and extracellular acidification rate), migration, and invasion of CaSki and SiHa cells. HPV‐16 E6/E7 knockdown in CaSki and SiHa cells had limited influence on PGK1 transcription but significantly decreased the half‐life of PGK1 protein. E6/E7 knockdown mediated PGK1 downregulation could be blocked by adding MG‐132. PGK1 poly‐ubiquitination was significantly enhanced after E6/E7 knockdown. In conclusion, this study showed that PGK1 expression might serve as a prognostic biomarker in cervical cancer. Its upregulation contributes to enhanced aerobic glycolysis, migration, and invasion of CESC cells. HPV16 E6/E7 stabilizes PGK1 protein by reducing its poly‐ubiquitination.

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Section: Introductionmentioning

confidence: 99%

“…| © 2021 International Federation for Cell Biology C Cell ell B Biology iology I International nternational (Vijaya Rachel & Sivaraj, 2021). One recent study noticed that HPV16 E6/E7 proteins could enhance the expression of hexokinase-ll (HK-II) (Zeng et al, 2017).…”

mentioning

confidence: 99%