Cervical Cancer HeLa Cell Autocrine Apoptosis Induced by Coimmobilized IFN-γ plus TNF-α Biomaterials

Li, Jian; Zhang, Yuxiao; Chen, Liyi; Lu, Xinhua; Li, Zhibin; Xue, Yongyong; Guan, Yan‐Qing

doi:10.1021/acsami.7b18277

Cited by 19 publications

(10 citation statements)

References 56 publications

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“…Studies showed that the proliferation and activation of host T cells was suppressed by IFN-γ indirectly, which protected mice against GVHD [62,93,94]. Moreover, IFN-γ was proven to have a positive therapeutic effect on human malignancies [95][96][97], and it could promote DNT cell-mediated killing of leukemia cells by facilitating NKG2D and DNAM-1 ligand expression [36]. Furthermore, IFN-γ increased Fas and FasL protein expression to inhibit leukemia K562 cell proliferation and promote cell apoptosis [98].…”

Section: Cytokinesmentioning

confidence: 99%

DNT Cell-based Immunotherapy: Progress and Applications

Li¹,

Dong²,

Fan³

et al. 2020

J. Cancer

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Cancer immunotherapy has firmly established a dominant status in recent years. Adoptive cellular immunotherapy (ACI) is the main branch of immunotherapy. Recently, the immune effector cells of ACI, such as T cells, NK cells, and genetically engineered cells, have been used to achieve significant clinical benefits in the treatment of malignant tumors. However, the clinical applications have limitations, including toxicity, unexpectedly low efficiency, high costs and strict technical requirements. More exploration is needed to optimize ACI for cancer patients. CD3+CD4-CD8-double negative T cells (DNTs) have emerged as functional antitumor effector cells, according to the definition of adoptive immunotherapy. They constitute a kind of T cell subset that mediates nontumor antigen-restricted immunity and has important immune regulatory functions. Preclinical experiments showed that DNTs had a dual effect by killing tumor cells and inhibiting graft-versus-host disease. Notably, DNTs can be acquired from healthy donors and expanded in vitro; thus, allogeneic DNTs may be provided as "off-the-shelf" cellular products that can be readily available for direct clinical application. We review the progress and application of DNTs in immunotherapy. DNTs may provide some novel perspectives on cancer immunotherapy.

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Section: Cytokinesmentioning

confidence: 99%

DNT Cell-based Immunotherapy: Progress and Applications

Li¹,

Dong²,

Fan³

et al. 2020

J. Cancer

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“…As a proinflammatory cytokine, TNFα had been reported to be associated with cell proliferation, differentiation, apoptosis, and immune response. Moreover, Li et al indicated that TNF-α induced the apoptosis of cervical cells ( Li J. et al, 2018 ). Rashmi et al suggested that AKT inhibitors suppress the proliferation of cervical cancer cells via disruption of mTOR signaling and glucose uptake ( Rashmi et al, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

Identify Function of WASL in Prognosis of Cervical Cancer Based on Omics Data

Hou

Chen

et al. 2021

Front. Cell Dev. Biol.

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ObjectiveTo clarify the clinical relevance of WASP like actin nucleation promoting factor (WASL) in patients with cervical cancer and associated mechanisms.Methods and MaterialsWe obtained high prediction accuracy and determined the correlation between the expression of WASL and the clinical characteristics of cervical cancer patients. Differentially expressed genes (DEGs) were identified using microarray. Gene ontology (GO) enrichment analysis and gene set enrichment analysis (GSEA) were performed to determine potentially relevant mechanisms related to the prognostication ability of WASL expression.ResultsChi-square test and multivariable logistic regression analysis suggested that lower expression of WASL was associated with lower pathological stage (chi-square test: p = 0.022, chi-square = 9.613; logistic regression: OR = 0.869, 95% CI: 0.756–0.991, p = 0.041). Patients in the WASL high expression group have worse overall survival (OS) [hazard ratio (HR): 0.555, 95% CI: 0.348–0.884, log-rank p = 0.012] and recurrence-free survival (RFS) (HR = 0.449, 95% CI: 0.215–0.934, log-rank p = 0.028) compared with those in the WASL low expression group. Univariate and multivariable Cox proportional hazards regression model suggested that WASL expression was an independent prognostic factor for predicting OS and RFS in cervical cancer. DEGs were mostly enriched GO terms related to DNA replication or the proliferation of tumor cells. The results of GSEA suggested samples in the WASL knockdown group were enriched in glycolysis, TNF-α signaling via NFkB, mTORC1 signaling, and Wnt/β-catenin signaling.ConclusionsWASL expression was associated with the pathological stage, and it might be an independent prognostication factor in patients with cervical cancer. Knockdown of WASL might be correlated with biological processes such as glycolysis, TNFα signaling, mTOR signaling, and Wnt/β-catenin signaling.

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“…All these experiments above confirmed that the EVO has powerful anti-cancer effects against gallbladder cancer. P38 MAPK pathway regulates various cellular activities concerned with cancer development, including proliferation, differentiation, apoptosis and inflammation [27,28] Tumor necrosis factor-α(TNF-α), which is major mediator of extrinsic apoptosis, was initially considered only to be secreted by inflammation-related cells such as macrophages, but more recent research has found that a wide variety of tumor cells can secrete TNF-α, including breast, ovarian, colon cancers [29]. P38 MAPK pathway can be activated by various types of cellular stress such as oxidative, genotoxic, and osmotic stress and maybe more importantly by pro-inflammatory cytokines such as TNF-α [27].…”

Section: Discussionmentioning

confidence: 99%

Evodiamine Induces Extrinsic Apoptosis Through P38 Mapk Pathway and Inhibits Autophagy in Gallbladder Cancer

Yang

et al. 2018

BJSTR

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The gallbladder cancer is the most common malignant tumor of the biliary tract and the third most common gastrointestinal malignant tumor in the world for public health with extremely poor prognosis. The primary purpose of this study is to investigate the inhibition effects of evodiamine, a major alkaloid compound extracted from the dry unripened fruit Evodiae fructus, on the carcinoma of the gallbladder cancer, and explored the underlying molecular mechanisms responsible for these effects. Cell viability of NOZ and GBC-SD cells was assessed by CCK-8 and colony forming assays. Cell apoptosis was analyzed by Annexin V-FITC and PI double staining flow cytometry and the apoptosis-related DNA damage was detected by Immunofluorescence microscopy assays. Western blot analysis was used to analyze the expression of crucial proteins involved in apoptosis, autophagy and signaling pathways. In this study, we found evodiamine induced cell viability and colony forming inhibition, apoptosis promotion and autophagy inhibition of NOZ and GBC-SD cells in a dose-dependent manner. The activation of p38 MAPK signaling pathway was significantly associated with evodiamine-treated apoptosis of gallbladder cancer cells, and the depletion of p53 was related with decreasing autophagy. Consequently, we propose that evodiamine may serve as a potential therapeutic agent for gallbladder cancer.

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Cervical Cancer HeLa Cell Autocrine Apoptosis Induced by Coimmobilized IFN-γ plus TNF-α Biomaterials

Cited by 19 publications

References 56 publications

DNT Cell-based Immunotherapy: Progress and Applications

DNT Cell-based Immunotherapy: Progress and Applications

Identify Function of WASL in Prognosis of Cervical Cancer Based on Omics Data

Evodiamine Induces Extrinsic Apoptosis Through P38 Mapk Pathway and Inhibits Autophagy in Gallbladder Cancer

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