Cervical anticancer activities of Annona squamosa Linn. leaf isolate

Abstract

Background and Aim: Cancer is one of the leading causes of death, the need for new anticancer herbal drugs is becoming more urgent considering the side effects of synthetic drugs. This study aimed to determine the anticancer activity of isolates derived from the methanol extract of Annona squamosa Linn. leaves and to identify the compounds that have an active effect against HeLa cells. Materials and Methods: The leaf metabolites of A. squamosa L. were extracted using methanol at room temperature (28°C) and were partitioned into n-hexane, chloroform, and n-butanol. The toxicity test of these extracts was conducted using a brine shrimp lethality assay. Furthermore, the most toxic extracts were separated and purified using silica gel column chromatography to yield four isolate fractions: FA, FB, FC, and FD. The most toxic isolates were tested for anticancer against HeLa cells, and their compounds were identified using liquid chromatography-mass spectrometry. Results: The results showed that the most toxic isolate with an LC50 value of 100.00 ppm had a potency similar to that of an anticancer agent with an IC50 value of 70.9021 ppm. Furthermore, the five compounds identified in this isolate include (6S, 7aR)-6-hydroxy-4,4,7a-trimethyl-6,7-dihydro-5H-1-benzofuran-2-one or loliolide, cocamidopropyl betaine, N-[3- (dimethylamino)propyl]dodecanamide or lauramidopropyl dimethylamine, linolenic acid, and 1-dodecyl-2-azepanone or laurocapram. Conclusion: It can be concluded that the leaf isolates of A. squamosa Linn. had shown anticancer activities against cervical cancer.

Phytochemical Study of Euphorbia turcomanica Boiss.

“…Studies have revealed anti-bacterial and anti-fungal properties [53] as well as anti-oxidant [54,55], anti-inflammatory [56,57], potential anti-diabetic [58], and neuromodulatory effects [59,60]. Many studies on plant extracts from other families containing loliolide have been carried out that proved a wide range of anti-cancer activities on different cell lines [61][62][63][64][65][66][67]. So far, loliolide has been isolated from other species of the Euphorbia genus, such as E. supina [68], E. micractina [69], E. cooperi [70], and E. alatavica [71] but has never been reported to be identified in E. turcomanica before.…”

Annona squamosa L.: A promising herbal remedy - Insights into its biological activities and phytochemical composition

“…Currently, Annona plants are receiving special attention for their potent effects in combating cancer. Researchers are particularly interested in the remarkable anticancer properties exhibited by various compounds found within Annona species (72,73,98) . Moreover, there is a notable increase in studies concerning the applications of nanoparticle drug delivery systems in combination with different A. squamosa extracts for different therapeutic purposes (99)(100)(101) .…”

Effect of the Methanol Extract of Annona squamosa Linn Leaf on Cervical Cancer

“…Toxicity test procedure followed Swantara et al (2022). Filtered seawater was used as a medium for hatching larvae.…”