Ceruloplasmin, serotransferrin and albumin presented different abundance in mares’ uterine fluid five days after insemination

Lancheros-Buitrago, Deisy J.; Rodríguez-Villamil, P.; Gregory, Joana; Bastos, Henrique Boll de Araujo; Camacho, Cesar Augusto; Caballeros, Jorge Emilio; Cazales, N.; Barros, Edvaldo; Silva, Maria José de Jesus; Pimentel, Ana Laura; Mattos, Rodrigo Costa

doi:10.1016/j.theriogenology.2019.11.011

Cited by 9 publications

(5 citation statements)

References 39 publications

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“…It possibly indicates the interaction of various proteins in the placentation, and secreted endometrial proteins to the blood. This coincides with the results in mare uterine fluid (Lancheros‐Buitrago et al, 2020; Smits et al, 2018), and jenny serum (Deng et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Quantitative analysis of the serum proteome during early pregnancy in mares

Liang

Zheng

Tang

et al. 2022

Animal Science Journal

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Equine pregnancy is currently diagnosed by rectal palpation, ultrasonographic examination, or by measuring changes in hormones in the blood. In the present study, we identified proteins that are differentially expressed in the sera of early pregnant and non-pregnant mares in order to develop a novel method for diagnosing equine pregnancy. Serum samples were obtained from 18 adult mares, pregnancy at day 32 after ovulation (n = 9) and in diestrus (n = 9). Proteomic analysis of the samples was conducted using liquid chromatography-electrospray ionization-tandem mass spectrometry. We identified 467 proteins from a total of 3514 peptides. Thirty-two proteins (15 upregulated and 17 downregulated) were significantly differentially expressed between the two groups. The Gene Ontology enrichment analysis revealed that they are related to extracellular matrix assembly, blood coagulation, and hemostasis, and the prominent molecular functions were integrin binding, cell adhesion molecule binding, and glycine C-acetyltransferase activity. The pathway analysis of Kyoto Encyclopaedia of Genes and Genomes showed that the top three pathways identified were glycine, serine, and threonine metabolism; cysteine and methionine metabolism; and ether lipid metabolism. The selected five serum proteins were newly potential candidates for pregnancy diagnosis in mares.

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Section: Discussionmentioning

confidence: 99%

Quantitative analysis of the serum proteome during early pregnancy in mares

Liang

Zheng

Tang

et al. 2022

Animal Science Journal

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“…Identification of biomarkers or tracking changes in sample chemical components may often signal the presence of physiological conditions requiring treatment regimens. Table-3 summarizes recent applications of MALDI-TOF for diagnostics based on molecular typing [138][139][140][141][142][143][144][145][146][147][148][149][150][151][152][153][154]. For the first example, Riengvirodkij et al [138] used MALDI-TOF in effort to identify peptide biomarkers associated with mitral valve disease in dogs.…”

Section: Matrix-assisted Laser Desorption Ionizationtime-of-flight Fo...mentioning

confidence: 99%

“…The study of protein expression related to animal reproduction is also an active area of research using MALDI-TOF. Lancheros-Buitrago et al [151] used MALDI-TOF to measure abundance of proteins present in inseminated and cyclic mares. The goal was to identify biomarkers of the potential equine conceptus.…”

Section: Matrix-assisted Laser Desorption Ionizationtime-of-flight Fo...mentioning

confidence: 99%

Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry in veterinary medicine: Recent advances (2019–present)

Thompson

2022

Vet World

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Matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry (MS) has become a valuable laboratory tool for rapid diagnostics, research, and exploration in veterinary medicine. While instrument acquisition costs are high for the technology, cost per sample is very low, the method requires minimal sample preparation, and analysis is easily conducted by end-users requiring minimal training. Matrix-assisted laser desorption ionization-time-of-flight MS has found widespread application for the rapid identification of microorganisms, diagnosis of dermatophytes and parasites, protein/lipid profiling, molecular diagnostics, and the technique demonstrates significant promise for 2D chemical mapping of tissue sections collected postmortem. In this review, an overview of the MALDI-TOF technique will be reported and manuscripts outlining current uses of the technology for veterinary science since 2019 will be summarized. The article concludes by discussing gaps in knowledge and areas of future growth.

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“…In clinical practice, blood Cp values are suitable for discriminating therapeutic treatments of colic syndrome during the acute-phase response; in fact, high blood Cp values suggest a surgical resolution, while physiological Cp values suggest a clinical treatment [26]. Interestingly, in inseminated mares, five days post-ovulation, Cp content in uterine fluid increased [27]. This occurrence could be traced to the endometrial preparation for recognition, attachment, and development of the embryo.…”

Section: Introductionmentioning

confidence: 99%

Analytical Validation of Two Assays for Equine Ceruloplasmin Ferroxidase Activity Assessment

Cecchini Gualandi,

Di Palma,

Boni

2023

Veterinary Sciences

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Ceruloplasmin (Cp) assessment in biological samples exploits the oxidase activity of this enzyme against several substrates, such as p-phenylenediamine (p-P), o-dianisidine (o-D) and, most recently, ammonium iron(II) sulfate (AIS). Once developed in humans, these assays are often used in veterinary medicine without appropriately optimizing in the animal species of interest. In this study, two assays using AIS and o-D as substrates have been compared and validated for Cp oxidase activity assessment in horse’s plasma. The optimization of the assays was performed mainly by varying the buffer pH as well as the buffer and the substrate molar concentration. Under the best analytical conditions obtained, the horse blood serum samples were treated with sodium azide, a potent Cp inhibitor. In the o-D assay, 500 µM sodium azide treatment completely inhibits the enzymatic activity of Cp, whereas, using the AIS assay, a residual analytical signal was still present even at the highest (2000 µM) sodium azide concentration. Even though the analytical values obtained from these methods are well correlated, the enzymatic activity values significantly differ when expressed in Units L−1. A disagreement between these assays has also been detected with the Bland–Altman plot, showing a progressive discrepancy between methods with increasing analytical values.

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Ceruloplasmin, serotransferrin and albumin presented different abundance in mares’ uterine fluid five days after insemination

Cited by 9 publications

References 39 publications

Quantitative analysis of the serum proteome during early pregnancy in mares

Quantitative analysis of the serum proteome during early pregnancy in mares

Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry in veterinary medicine: Recent advances (2019–present)

Analytical Validation of Two Assays for Equine Ceruloplasmin Ferroxidase Activity Assessment

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