Ceruloplasmin decreases respiratory burst reaction during pregnancy

Varfolomeeva, E. Yu.; Семенова, Е. В.; Sokolov, A. V.; Aplin, Kirill D; Timofeeva, Kseniya E; Vasilyev, V. B.; Филатов, М. В.

doi:10.1080/10715762.2016.1197395

Cited by 21 publications

(10 citation statements)

References 64 publications

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“…CP, медь-содержащий белок плазмы крови, также ингибирует хлорирующую активность MPO за счет образования комплекса, блокируя активный центр фермента [7]. Более того, даже при модификации продуктами катализа MPO (HOCl или HOBr) ингибирующая активность CP сохраняется, в том числе по отношению к подавлению активности нейтрофилов [19,20]. Из табл.…”

Section: результаты и их обсуждениеunclassified

Celestine blue B as a sensor for hypochlorous acid and HOCL-modified proteins registration

Луценко

Евгеньевна²,

Grigorieva

et al. 2019

Medical academic journal

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Objective — the study of hypochlorous acid (HOCl) and its derivatives production, which catalyzed by human neutrophil myeloperoxidase, using “turn-on” fluorescent sensor — celestine blue B. Materials and methods. Neutrophils were isolated from the venous blood of healthy donors. Phorbol 12-myristate 13-acetate, N-formyl-methionyl-leucyl-phenylalanine, plant lectins, HOCl-modified proteins were used as agonists. N-acetylcysteine, 4-aminobenzoic acid hydrazide, isoniazid and ceruloplasmin were used as regulators of neutrophil myeloperoxidase activity and/or HOCl scavengers. Results. Using a wide range of agonists and inhibitors, it has been shown that celestine blue B is oxidized in vitro by HOCl and its derivatives as a result of neutrophil myeloperoxidase activity. The oxidation of celestine blue B by HOCl-modified human serum albumin (HSA-Cl) and inhibition of this process by monoclonal antibody against HSA-Cl (IgM class) was also found. Conclusion. Based on the developed method using celestine blue B, it is possible to conduct a sensitive analysis for the presence of HOCl-modified proteins (chloramines, etc.), to investigate the effect of various agonists and drugs on myeloperoxidase activity and exocytosis from the neutrophil granules.

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Section: результаты и их обсуждениеunclassified

Celestine blue B as a sensor for hypochlorous acid and HOCL-modified proteins registration

Луценко

Евгеньевна²,

Grigorieva

et al. 2019

Medical academic journal

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“…В лунки помещали аффинные антитела крыс против CP (50 мкг/мл), через 60 минут планшет отмывали 3 раза PBS, содержащим 0,05%-ый Tween 20. В лунки помещали конъюгат антител против IgG крыс с пероксидазой из корней хрена (1:5000), через 60 минут планшет отмывали 3 раза PBS, содержащим 0,05%-ый Tween [35]. В лунки 96-луночного планшета вносили по 10 мкл проб сыворотки крови либо калибровочных растворов, содержащих 100-1300 мкг/мл очищенного CP [13], во все лунки добавляли по 200 мкл 0,2% p-фенилендиамина в 0,4 М Na-ацетатном буфере, рН 5,5.…”

Section: получение антител против Cp и Cp-hocl а также выявление Cp-unclassified

“…MPO содержится главным образом в азурофильных гранулах нейтрофилов и в моноцитах, составляя около 5 и 0,9% от общего белка этих клеток соответственно [25]. При взаимодействии с H 2 O 2 происходит превращение MPO в высокореакционное Соединение I, которое, наряду с классическим одноэлектронным окислением пероксидазных субстратов (например, о-дианизидина, тирозина [3,35]), обладает уникальной способностью катализировать двухэлектронное окисление хлорид-иона с образованием хлорноватистой кислоты (HOCl). Образование HOCl в описанном выше галогенирующем цикле функционирования MPO считается важным фактором врожденного иммунитета.…”

Section: Introductionunclassified

A Link Between Active Myeloperoxidase and Chlorinated Ceruloplasmin in Blood Plasma of Patients With Cardiovascular Diseases

et al. 2018

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Myeloperoxidase is a key factor promoting development of halogenative/oxidative stress under inflammatory conditions. Previously, we have discovered complexes including myeloperoxidase and its physiological inhibitor, ceruloplasmin in blood plasma of patients with inflammatory diseases of different etiology, e.g., atherosclerosis. Studies on regulation of myeloperoxidase activity by ceruloplasmin have shown that hypochlorous acid, a specific product of myeloperoxidase action, is likely to modify ceruloplasmin during inflammation. The present study was aimed for analysis of relationships between the myeloperoxidase activity, native, and HOCl-modified ceruloplasmin levels in blood plasma samples of the patients with cardiovascular diseases.Specific antibodies against myeloperoxidase, ceruloplasmin, and HOCl-modified ceruloplasmin were obtained and specific enzyme-linked immunosorbent assays (ELISA) were developed. A combination of highly sensitive methods of myeloperoxidase assay i.e., solid-phase adsorption of antigens with subsequent testing of either their activity, or peroxidase-labeled antibody activity allowed elaborating the highly sensitive assays for ceruloplasmin and its HOCl-modified molecules, and for myeloperoxidase (concentration, peroxidase and halogenating activity). Positive correlation was proven between the myeloperoxidase concentration and activities. HOCl-modified ceruloplasmin content also correlated with myeloperoxidase activity.The HOCl-modified ceruloplasmin was first discovered in blood plasma samples from patients with cardiovascular diseases. In view of correlation between myeloperoxidase activity and HOCl-modified ceruloplasmin content in plasma, we suggest that HOCl production is aimed for suppression of myeloperoxidaseinhibitory function of ceruloplasmin.

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“…Further to this direct antioxidant action, CP can affect protective enzymatic mechanisms, thus moderating immune responses [ 6 ]. Indeed, as the plasma concentration of this copper-containing protein increases from approximately 200–900 µg/mL in response to inflammation, CP refers to the positive acute-phase proteins.…”

Section: Introductionmentioning

confidence: 99%

Fine Regulation of Neutrophil Oxidative Status and Apoptosis by Ceruloplasmin and Its Derivatives

Golenkina

Viryasova

Galkina

et al. 2018

Cells

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Timely neutrophil apoptosis is an essential part of the resolution phase of acute inflammation. Ceruloplasmin, an acute-phase protein, which is the predominant copper-carrying protein in the blood, has been suggested to have a marked effect on neutrophil life span. The present work is a comparative study on the effects of intact holo-ceruloplasmin, its copper-free (apo-) and partially proteolyzed forms, and synthetic free peptides RPYLKVFNPR (883–892) and RRPYLKVFNPRR (882–893) on polymorphonuclear leukocyte (PMNL, neutrophil) oxidant status and apoptosis. The most pronounced effect on both investigated parameters was found with copper-containing samples, namely, intact and proteolyzed proteins. Both effectively reduced spontaneous and tumor necrosis factor-α (TNF-α)-induced extracellular and intracellular accumulation of superoxide radicals, but induced a sharp increase in the oxidation of intracellular 2′,7′-dichlorofluorescein upon short exposure. Therefore, intact and proteolyzed ceruloplasmin have both anti- and pro-oxidant effects on PMNLs wherein the latter effect is diminished by TNF-α and lactoferrin. Additionally, all compounds investigated were determined to be inhibitors of delayed spontaneous apoptosis. Intact enzyme retained its pro-survival activity, whereas proteolytic degradation converts ceruloplasmin from a mild inhibitor to a potent activator of TNF-α-induced neutrophil apoptosis.

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Ceruloplasmin decreases respiratory burst reaction during pregnancy

Cited by 21 publications

References 64 publications

Celestine blue B as a sensor for hypochlorous acid and HOCL-modified proteins registration

Celestine blue B as a sensor for hypochlorous acid and HOCL-modified proteins registration

A Link Between Active Myeloperoxidase and Chlorinated Ceruloplasmin in Blood Plasma of Patients With Cardiovascular Diseases

Fine Regulation of Neutrophil Oxidative Status and Apoptosis by Ceruloplasmin and Its Derivatives

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