Cerium(<scp>iv</scp>) oxide nanoparticles induce sublethal changes in honeybees after chronic exposure

Kos, Monika; Kokalj, Anita Jemec; Glavan, Gordana; Marolt, Gregor; Zidar, Primož; Božič, Janko; Novak, Sara; Drobne, Damjana

doi:10.1039/c7en00596b

Cited by 17 publications

(11 citation statements)

References 81 publications

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“…Among its numerous biological applications is antibacterial, antioxidant, and anti-inflammatory activities (Heckert et al, 2008;Pirmohamed et al, 2010;Varini et al, 2019;Walkey et al, 2015;Zhang et al, 2019). Due to the structural characteristics of the nanoceria, it combines with soil particles and remains in the soil, so it is also likely to participate in physical and biological cycles (Kos et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

In vitro genotoxicity assessment and comparison of cerium (IV) oxide micro- and nanoparticles

Arslan

Akbaba

2020

Toxicol Ind Health

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Cerium (IV) oxide (CeO2), which is used as a biomaterial, has wide application in areas such as the biomedical, glass polishing, electronic, automotive, and pharmacology industries. Comparing with the literature, in this study, the genotoxic effects of cerium (IV) oxide microparticles (COMPs) and cerium (IV) oxide nanoparticles (CONPs) were investigated for the first time in human peripheral blood cultures at concentrations of 0.78, 1.56, 3.125, 6.25, 12.5, 25, and 50 ppm for 72 h under in vitro conditions. Particle sizes of COMPs and CONPs were determined using scanning electron microscopic analysis. Micronucleus and chromosome aberration tests were used to determine the genotoxicity of COMPs and CONPs. The average particle sizes of COMPs and CONPs were approximately 148.25 and 25.30 nm, respectively. It was determined that CeO2 particles in both micro and nano sizes were toxic at all concentrations compared to the negative control group (distilled water). Importantly, COMPs and CONPs were genotoxic even at the lowest concentration (0.78 ppm). Comparing particle sizes, the data indicated that COMPs were more toxic than CONPs. The results suggest that genotoxicity of COMPs and CONPs may be a function of applied concentrations and particle sizes.

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Section: Introductionmentioning

confidence: 99%

In vitro genotoxicity assessment and comparison of cerium (IV) oxide micro- and nanoparticles

Arslan

Akbaba

2020

Toxicol Ind Health

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“…The mean particle diameter (TEM) was 4.7 ± 1.4 nm (JEOL JEM2100F, Tokyo, Japan), the Z-average size was 172.1 ± 1.705 nm, the polydispersity index (PDI) was 0.272 ± 0.009, and the zeta potential was 50.3 ± 0.719 mV (Malvern Zetasizer 5000, Malvern Panalytical Ltd, Worcester, UK). Further particle characterisation can be found in Kos et al (2017) [66].…”

Section: Nanoparticle Synthesis and Characterisationmentioning

confidence: 99%

“…Compared to Au NPs, they are more often considered as biologically potent and can induce oxidative stress; inflammation; DNA damage; and affect reproductive capability, growth, and the survival of exposed animals [59,60]. In the study of Kos et al (2017), honeybees were exposed to the very same CeO 2 NPs as in the present study. Significant alterations in Acetylcholinesterase and glutathione-S-transferase activities were evidenced at concentration 2 mg/L.…”

Section: Introductionmentioning

confidence: 98%

Stressor-Dependant Changes in Immune Parameters in the Terrestrial Isopod Crustacean, Porcellio scaber: A Focus on Nanomaterials

et al. 2021

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We compared the changes of selected immune parameters of Porcellio scaber to different stressors. The animals were either fed for two weeks with Au nanoparticles (NPs), CeO2 NPs, or Au ions or body-injected with Au NPs, CeO2 NPs, or lipopolysaccharide endotoxin. Contrary to expectations, the feeding experiment showed that both NPs caused a significant increase in the total haemocyte count (THC). In contrast, the ion-positive control resulted in a significantly decreased THC. Additionally, changes in phenoloxidase (PO)-like activity, haemocyte viability, and nitric oxide (NO) levels seemed to depend on the stressor. Injection experiments also showed stressor-dependant changes in measured parameters, such as CeO2 NPs and lipopolysaccharide endotoxin (LPS), caused more significant responses than Au NPs. These results show that feeding and injection of NPs caused an immune response and that the response differed significantly, depending on the exposure route. We did not expect the response to ingested NPs, due to the low exposure concentrations (100 μg/g dry weight food) and a firm gut epithelia, along with a lack of phagocytosis in the digestive system, which would theoretically prevent NPs from crossing the biological barrier. It remains a challenge for future research to reveal what the physiological and ecological significance is for the organism to sense and respond, via the immune system, to ingested foreign material.

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“…Once soil was contaminated by nano-La 2 O 3 , plants can serve as a potential intermediary pathway that could bioaccumulate and transport them to primary consumers, for example, Acheta domestica, Tenebrionoidea, and honeybees (Ma et al, 2011;De la Torre Roche et al, 2015). Related studies have also demonstrated that honeybees come in contact with metal oxide NPs (e.g., CeO 2 , CdO, and PbO) through surface particle adhesion, dust inhalation, foraging on contaminated food, or water (Kos et al, 2017;Al Naggar et al, 2020). Therefore, honeybees may suffer the environmental exposure of terrestrial REO NPs and serve as sensitive indicators of environmental quality.…”

Section: Introductionmentioning

confidence: 99%

“…Due to their extensive social activities within 14km 2 foraging areas, honeybees are exposed to conventional contaminants, for example, pesticides, antibiotics, and respirable suspended particulate matters, that may lead to the decline of honeybee colonies or the deterioration of honeybee health status ( Bargańska et al, 2016 ; Thimmegowda et al, 2020 ; Bondarenko et al, 2021 ; Kapoor et al, 2021 ). Among the emerging contaminants, rare earth oxide (REO) nanoparticles (NPs), characterized by their unique chemical and physical properties, have been one of most widely used materials in various industries and biotechnology applications ( Mastronardi et al, 2015 ; Gao et al, 2017 ; Kos et al, 2017 ). For example, the fertilizers, pesticides, and germination stimulants containing or engineered with REO NPs have been widely used to enhance the efficiency and sustainability of agricultural practices, and nano-La 2 O 3 is reported to account for approximately 30% of REO NPs additives and have higher cytotoxicity compared with other REO NPs ( De la Torre Roche et al, 2015 ; Mastronardi et al, 2015 ; Servin et al, 2015 ; Gao et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Nano-La2O3 Induces Honeybee (Apis mellifera) Death and Enriches for Pathogens in Honeybee Gut Bacterial Communities

et al. 2021

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Honeybees (Apis mellifera) can be exposed via numerous potential pathways to ambient nanoparticles (NPs), including rare earth oxide (REO) NPs that are increasingly used and released into the environment. Gut microorganisms are pivotal in mediating honeybee health, but how REO NPs may affect honeybee health and gut microbiota remains poorly understood. To address this knowledge gap, honeybees were fed pollen and sucrose syrup containing 0, 1, 10, 100, and 1000mgkg−1 of nano-La2O3 for 12days. Nano-La2O3 exerted detrimental effects on honeybee physiology, as reflected by dose-dependent adverse effects of nano-La2O3 on survival, pollen consumption, and body weight (p<0.05). Nano-La2O3 caused the dysbiosis of honeybee gut bacterial communities, as evidenced by the change of gut bacterial community composition, the enrichment of pathogenic Serratia and Frischella, and the alteration of digestion-related taxa Bombella (p<0.05). There were significant correlations between honeybee physiological parameters and the relative abundances of pathogenic Serratia and Frischella (p<0.05), underscoring linkages between honeybee health and gut bacterial communities. Taken together, this study demonstrates that nano-La2O3 can cause detrimental effects on honeybee health, potentially by disordering gut bacterial communities. This study thus reveals a previously overlooked effect of nano-La2O3 on the ecologically and economically important honeybee species Apis mellifera.

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Cerium(iv) oxide nanoparticles induce sublethal changes in honeybees after chronic exposure

Abstract: Oral exposure to up to 500 mg L−1 nCeO2 was sublethal for honeybees but altered the cholinergic system and induced other physiological responses.

Cited by 17 publications

References 81 publications

In vitro genotoxicity assessment and comparison of cerium (IV) oxide micro- and nanoparticles

In vitro genotoxicity assessment and comparison of cerium (IV) oxide micro- and nanoparticles

Stressor-Dependant Changes in Immune Parameters in the Terrestrial Isopod Crustacean, Porcellio scaber: A Focus on Nanomaterials

Nano-La2O3 Induces Honeybee (Apis mellifera) Death and Enriches for Pathogens in Honeybee Gut Bacterial Communities

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