Cerium Oxide Nanoparticles Promote Osteoplastic Precursor Differentiation by Activating the Wnt Pathway

Luo, Junchao; Zhu, Senbo; Zhang, Yin; Li, Yong; Cao, Li; Kong, Mingxiang; Luo, Meihui; Bi, Qing; Zhang, Qiong

doi:10.1007/s12011-022-03168-9

Cited by 19 publications

(20 citation statements)

References 23 publications

(25 reference statements)

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“…The early induction of differentiation of hPDLCs cultured with the ART-loaded SiCaCe NPs, as indicated by the increased ALP activity in day 14, and is followed by a decrease in ALP activity after 21 days in culture, during mineral deposition. Our results agree with the studies from Stein and Lian, and Choi et al, who showed that the peak of ALP expression was observed during the post-proliferative period, and cells stained positive for ALP during the early mineralization stage, were followed by the decreased ALP expression during the heavy mineralization stage [ 43 , 44 ]. Assessment of the ALP expression at the mRNA level might show the peak expression at an earlier timepoint [ 47 , 48 ].…”

Section: Discussionsupporting

confidence: 93%

“…Nonetheless, Ce-NPs bioactivity and ability to reduce ROS should not be solely attributed to its redox states, but can also be related to components of Ce-NPs surface chemistry, such as particle morphology and surface modification [ 43 ]. Luo et al demonstrated that Ce-NPs promoted the differentiation of precursor osteoblasts by enabling the nuclear translocation of β-catenin protein and activation of the Wnt pathway [ 44 ]. Lu et al showed that incorporation of Ce-NPs in hollow mesoporous bioglass scaffolds activates the ERK pathway, thus stimulating the osteogenic differentiation of bone marrow MSCs [ 45 ].…”

Section: Discussionmentioning

confidence: 99%

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Artemisinin Loaded Cerium-Doped Nanopowders Improved In Vitro the Biomineralization in Human Periodontal Ligament Cells

et al. 2023

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Background: A promising strategy to enhance bone regeneration is the use of bioactive materials doped with metallic ions with therapeutic effects and their combination with active substances and/or drugs. The aim of the present study was to investigate the osteogenic capacity of human periodontal ligament cells (hPDLCs) in culture with artemisinin (ART)-loaded Ce-doped calcium silicate nanopowders (NPs); Methods: Mesoporous silica, calcium-doped and calcium/cerium-doped silicate NPs were synthesized via a surfactant-assisted cooperative self-assembly process. Human periodontal ligament cells (hPDLCs) were isolated and tested for their osteogenic differentiation in the presence of ART-loaded and unloaded NPs through alkaline phosphatase (ALP) activity and Alizarine red S staining, while their antioxidant capacity was also evaluated; Results: ART promoted further the osteogenic differentiation of hPDLCs in the presence of Ce-doped NPs. Higher amounts of Ce in the ART-loaded NPs inversely affected the mineral deposition process by the hPDLCs. ART and Ce in the NPs have a synergistic role controlling the redox status and reducing ROS production from the hPDLCs; Conclusions: By monitoring the Ce amount and ART concentration, mesoporous NPs with optimum properties can be developed towards bone tissue regeneration demonstrating also potential application in periodontal tissue regeneration strategies.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Artemisinin Loaded Cerium-Doped Nanopowders Improved In Vitro the Biomineralization in Human Periodontal Ligament Cells

et al. 2023

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“…These unique XRD peaks were defined by their respective indices (111), ( 200), (220), (311), ( 222), (400), and (420). 40,41 The diffraction peaks of Gel and Alg mixed with nCeO 2 broadened significantly and moved to lower 2θ. The addition of GA caused the peaks of the GA-nCeO 2 hydrogel to weaken at the first peak while enhancing the second peak, indicating a phase transition during the composite process.…”

Section: Characterization Of Ga-nceo 2 Hydrogel Scaffoldmentioning

confidence: 99%

Alginate/Gelatin Hydrogel Scaffold Containing nCeO2 as a Potential Osteogenic Nanomaterial for Bone Tissue Engineering

Li¹,

Li²,

Song³

et al. 2022

IJN

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Background: Clinicians frequently face difficulties when trying to fix bone abnormalities. Gelatin-Alginate (GA) is frequently employed as a carrier because it is non-toxic, biodegradable, and has a three-dimensional network structure. Meanwhile, cerium oxide nanoparticles (nCeO 2 ) demonstrated high antioxidant enzyme simulation activity. Therefore, in order to develop a porous hydrogel scaffold for the application of bone tissue engineering, an appropriate-type GA-nCeO 2 hydrogel scaffold was developed and evaluated. Methods: GA-nCeO 2 hydrogel scaffold was prepared by the lyophilized method and characterized. The surface morphology and cell adhesion of the scaffold were observed by the scanning electron microscope. CCK8 and live-dead staining methods were used to evaluate its biological safety and cell proliferation. Then the osteogenic differentiation in early and late stages was discussed. The expression of osteogenic genes was also detected by RT-PCR. Finally, a bone defect model was made in SD rats, and bone formation in vivo was detected. Results:The results showed that GA-nCeO 2 hydrogel scaffold exhibited a typical three-dimensional porous structure with a mean pore ratio of 70.61 ± 1.94%. The GA-nCeO 2 hydrogel was successfully endowed with simulated enzyme activity including superoxide dismutase (SOD) and catalase (CAT) after the addition of nCeO 2 . Osteoblasts demonstrated superior cell proliferation and adhesion on composite scaffolds, and both mineralization test and gene expression demonstrated the strong osteogenic potential of GA-nCeO 2 hydrogel. The outcomes of hematoxylin and eosin (H&E) staining and Masson trichrome staining in the femoral defect model of SD rats further supported the scaffold's favorable biocompatibility and bone-promoting capacity. Conclusion: Due to its favorable safety, degradability, and bone formation property, GA-nCeO 2 hydrogel was anticipated to be used as a potential bone defect healing material.

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“…The addition of CeO 2 NPs displays protective effect toward oxidative stress in bone defects. More importantly, CeO 2 NPs have been reported to promote proliferation and osteogenic and adipogenic differentiation of different mesenchymal stem cells and osteoblasts. − Under inflammatory conditions, CeO 2 NPs can also regulate the polarization of macrophages and reduce the inflammation expression . Nevertheless, the application of CeO 2 NPs in bone regeneration area is still in its infancy, and multifunctional application scenarios, systematic research, and mechanism studies are of great urgency.…”

Section: Introductionmentioning

confidence: 99%

Sequential Therapy for Bone Regeneration by Cerium Oxide-Reinforced 3D-Printed Bioactive Glass Scaffolds

Zhang

Zhai

et al. 2023

ACS Nano

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Rational design of multifunctional biomaterials with customized architecture and on demand bioactivity is of great significance for bone tissue engineering (BTE) in modern society. Herein, a versatile therapeutic platform has been established by integrating cerium oxide nanoparticles (CeO 2 NPs) into bioactive glass (BG) to fabricate three-dimensional (3D)-printed scaffolds, achieving a sequential therapeutic effect against inflammation and promoting osteogenesis toward bone defect. The antioxidative activity of CeO 2 NPs plays a crucial role in alleviating the oxidative stress upon formation of bone defects. Subsequently, CeO 2 NPs exert a promotion effect on the proliferation and osteogenic differentiation of rat osteoblasts through enhancing mineral deposition and alkaline phosphatase and osteogenic gene expression. Strikingly, the incorporation of CeO 2 NPs bestows on the BG scaffolds greatly reinforced mechanical properties, improved biocompatibility, adequate cell adhesion, elevated osteogenic capability, and multifunctional performance in a single platform. In vivo studies on the treatment of rat tibial defect confirmed the better osteogenic properties of CeO 2 −BG scaffolds compared with pure BG scaffolds. Additionally, the employment of the 3D printing technique creates a proper porous microenvironment around the bone defect, which further facilitates the cell ingrowth and new bone formation. This report provides a systematic study on CeO 2 −BG 3D-printed scaffolds prepared by simple ball milling method, achieving sequential and integral treatment in BTE based on a single platform.

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Cerium Oxide Nanoparticles Promote Osteoplastic Precursor Differentiation by Activating the Wnt Pathway

Cited by 19 publications

References 23 publications

Artemisinin Loaded Cerium-Doped Nanopowders Improved In Vitro the Biomineralization in Human Periodontal Ligament Cells

Artemisinin Loaded Cerium-Doped Nanopowders Improved In Vitro the Biomineralization in Human Periodontal Ligament Cells

Alginate/Gelatin Hydrogel Scaffold Containing nCeO2 as a Potential Osteogenic Nanomaterial for Bone Tissue Engineering

Sequential Therapy for Bone Regeneration by Cerium Oxide-Reinforced 3D-Printed Bioactive Glass Scaffolds

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