Cerium oxide nanoparticle treatment ameliorates peritonitis-induced diaphragm dysfunction

Asano, Shigetaka; Arvapalli, Ravikumar; Manne, Nandini D.P.K.; Maheshwari, Mani; Ma, Bing; Rice, Kevin M.; Selvaraj, Vellaisamy; Blough, Eric R.

doi:10.2147/ijn.s89783

Cited by 14 publications

(13 citation statements)

References 45 publications

(58 reference statements)

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“…Previous studies have confirmed that CeO2 nanoparticles can act as superoxide dismutase mimics [4] and catalase mimics [5] and can reduce pulmonary hypertension [6] and reduce hypoxia-induced oxidative stress [7] as well as inhibiting cyclophosphamide-induced apoptosis [8]. In the study of peritonitis, CeO2 nanoparticles have been reported to reduce peritonitis-related SIRS [2], and other studies have found that CeO2 nanoparticles can improve diaphragmatic dysfunction caused by peritonitis [9]. In addition, green tea polyphenols, as the main ingredients of antioxidants, are well known for their antioxidant, anti-inflammatory, anticancer, anticardiolipin, antimicrobial, antihyperglycemic, and antiobesity properties [10].…”

Section: Introductionmentioning

confidence: 97%

Anti-Inflammatory Effects of Cerium Dioxide Nanoparticles on Peritonitis in Rats Induced by Staphylococcus epidermidis Infection

Sun

Yin

et al. 2020

Advances in Polymer Technology

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Objective. To investigate the effects of cerium dioxide (CeO2) nanoparticles on the inflammatory response of peritonitis rats induced by Staphylococcus epidermidis infection. Methods. Green tea polyphenol CeO2 nanoparticles were synthesized and characterized by transmission microscopy, ultraviolet-visible spectroscopy, FT-IR, and powder diffractometer. 40 male adult SD rats were randomly divided into 4 groups (n = 10 each): a control group, a model group, a CeO2 group, and a CeO2 + model group. Staphylococcus epidermidis solution was injected intraperitoneally with 107 CFU/ml of bacterial solution in the model group, while the control group was injected intraperitoneally with the same amount of normal saline, and the CeO2 and CeO2 + model groups were injected with 0.5 mg/kg CeO2 nanoparticles through the tail vein for 2 h and then injected with saline or bacterial solution for 2 h, respectively. After 0 h, 3 h, 12 h, 24 h, and 48 h of model construction, rats were sacrificed, and serum and peritoneal lavage fluid were collected. The total number of leukocytes and the percentage of each type of leukocytes in the peritoneal lavage fluid were determined. Enzyme-linked immunosorbent assay (ELISA) was used to detect the level of inflammatory factor TNF-α in serum and peritoneal lavage fluid, and myeloperoxidase (MPO) activity in peritoneal tissue was also measured. In addition, real-time fluorescence quantitative PCR (RT-PCR) was used to measure the expression of TLR2 and TLR4 in peritoneal tissue, and western blotting was used to detect the expression of TLR2, TLR4, and the activation of NF-κB signaling pathways as well. Results. The CeO2 has an average size of 37 ± 3 nm with binding activity to proteins, phenolic compounds, and alkaloids. After counting the white blood cells in the peritoneal lavage fluid, it was found that the total number of white blood cells and the percentage of neutrophils in the model group were significantly increased (both P<0.05), and CeO2 treatment significantly reversed the above changes (both P<0.05). The ELISA results showed that compared with the control group, the TNF-α in the peritoneal lavage fluid and serum of the model group increased in a time-dependent manner (all P<0.05); however, there was no significant change in the CeO2 group (P>0.05); at the same time in the CeO2 + model group, the TNF-α content was significantly reduced (all P<0.05). Detection of MPO activity in peritoneal tissue revealed that MPO activity was significantly increased under peritonitis (all P<0.05), and CeO2 treatment could mitigate that increase (all P<0.05). RT-PCR results showed that compared with the control group, the expression of TLR2 and TLR4 mRNA levels in the peritoneum of the model group were increased in a time-dependent manner (all P<0.05), and there was no significant change in the CeO2 group (P>0.05); however, TLR2 and TLR4 mRNA levels were significantly reduced in the CeO2 + model group (all P<0.05). Western blotting test was performed on the peritoneal tissue collected after 48 h of the model establishment. Compared with the control group, the levels of TLR2, TLR4, p–NF–κB, and p-IκBα protein in the model group were significantly increased (all P<0.05), while CeO2 group showed no significant changes (P>0.05) and administration of CeO2 before model construction can significantly reverse the above protein activation (all P<0.05). Conclusion. CeO2 nanoparticles have anti-inflammatory effects in peritonitis caused by Staphylococcus epidermidis infection.

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Section: Introductionmentioning

confidence: 97%

Anti-Inflammatory Effects of Cerium Dioxide Nanoparticles on Peritonitis in Rats Induced by Staphylococcus epidermidis Infection

Sun

Yin

et al. 2020

Advances in Polymer Technology

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“…The ceria nanozyme significantly improved survival and protected the liver from sepsis-induced injury. Similarly, other studies have also shown that ceria nanozymes can reduce functional sepsis-induced injury to the liver and diaphragm through their antioxidant and anti-inflammatory activities [ 89 , 90 ].…”

Section: Nanomaterials For Rons Eliminationmentioning

confidence: 92%

“… [ 72 ] TD-NT High polymer materials / PAMPs/DAMPs Decreased inflammatory cytokines levels and improved tissue damage in sepsis mice Captured DAMPs and PAMPs simultaneously / [ 74 ] Nanomaterials for RONS Elimination CeNPs Ceria / Antioxidant enzyme Decreased RONS and inflammatory cytokines levels; improved survival rate and protected liver from sepsis-induced injury / Large doses of ceria oxide nanoparticles may induce liver injury and oxidative stress. [ 88 ] / Improved diaphragmatic inflammation and function / [ 89 ] / Inhibited inflammatory cytokines secretion; alleviated hepatic damage; increased survival rate / [ 90 ] CZ-NPs Cerium and Zirconia / Antioxidant enzyme Exhibited a decreased RONS levels and an improvement of tissues damage in sepsis mice Exhibiting a stronger antioxidant activity Large doses of ceria oxide nanoparticles may induce liver injury and oxidative stress. [ 91 ] 6-AHA-CeNPs 6-AHA-modified cerium oxide / Antioxidant enzyme Exerted antioxidant and anti-inflammatory capacity; increased survival rates; improved lung and liver tissues damage Increasing stability and bioavailability of ceria nanozyme; decreasing toxicity Large doses of ceria oxide nanoparticles may induce liver injury and oxidative stress.…”

Section: Introductionmentioning

confidence: 99%

Nanotherapies for sepsis by regulating inflammatory signals and reactive oxygen and nitrogen species: New insight for treating COVID-19

et al. 2021

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SARS-CoV-2 has caused up to 127 million cases of COVID-19. Approximately 5% of COVID-19 patients develop severe illness, and approximately 40% of those with severe illness eventually die, corresponding to more than 2.78 million people. The pathological characteristics of COVID-19 resemble typical sepsis, and severe COVID-19 has been identified as viral sepsis. Progress in sepsis research is important for improving the clinical care of these patients. Recent advances in understanding the pathogenesis of sepsis have led to the view that an uncontrolled inflammatory response and oxidative stress are core factors. However, in the traditional treatment of sepsis, it is difficult to achieve a balance between the inflammation, pathogens (viruses, bacteria, and fungi), and patient tolerance, resulting in high mortality of patients with sepsis. In recent years, nanomaterials mediating reactive oxygen and nitrogen species (RONS) and the inflammatory response have shown previously unattainable therapeutic effects on sepsis. Despite these advantages, RONS and inflammatory response-based nanomaterials have yet to be extensively adopted as sepsis therapy. To the best of our knowledge, no review has yet discussed the pathogenesis of sepsis and the application of nanomaterials. To help bridge this gap, we discuss the pathogenesis of sepsis related to inflammation and the overproduction RONS, which activate pathogen-associated molecular pattern (PAMP)-pattern recognition receptor (PRR) and damage-associated molecular pattern (DAMP)-PRR signaling pathways. We also summarize the application of nanomaterials in the treatment of sepsis. As highlighted here, this strategy could synergistically improve the therapeutic efficacy against both RONS and inflammation in sepsis and may prolong survival. Current challenges and future developments for sepsis treatment are also summarized.

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“…15 a). The antioxidant effect of conventional CeO 2 nanoparticles is achieved with the chemical transformation of Ce 3+ to Ce 4+ , which partially attenuates oxide stress and proinflammatory responses in mice with sepsis [ 231 , 232 ]. However, the low reprocessing of Ce 3+ limits the therapeutic efficiency of CeO 2 nanoparticles and enhances their toxicity due to the large doses used.…”

Section: Nanotherapeutic Platforms For Sepsis Treatment Through Targementioning

confidence: 99%

Nanoplatforms for Sepsis Management: Rapid Detection/Warning, Pathogen Elimination and Restoring Immune Homeostasis

et al. 2021

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Sepsis, a highly life-threatening organ dysfunction caused by uncontrollable immune responses to infection, is a leading contributor to mortality in intensive care units. Sepsis-related deaths have been reported to account for 19.7% of all global deaths. However, no effective and specific therapeutic for clinical sepsis management is available due to the complex pathogenesis. Concurrently eliminating infections and restoring immune homeostasis are regarded as the core strategies to manage sepsis. Sophisticated nanoplatforms guided by supramolecular and medicinal chemistry, targeting infection and/or imbalanced immune responses, have emerged as potent tools to combat sepsis by supporting more accurate diagnosis and precision treatment. Nanoplatforms can overcome the barriers faced by clinical strategies, including delayed diagnosis, drug resistance and incapacity to manage immune disorders. Here, we present a comprehensive review highlighting the pathogenetic characteristics of sepsis and future therapeutic concepts, summarizing the progress of these well-designed nanoplatforms in sepsis management and discussing the ongoing challenges and perspectives regarding future potential therapies. Based on these state-of-the-art studies, this review will advance multidisciplinary collaboration and drive clinical translation to remedy sepsis."Image missing"

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Cerium oxide nanoparticle treatment ameliorates peritonitis-induced diaphragm dysfunction

Cited by 14 publications

References 45 publications

Anti-Inflammatory Effects of Cerium Dioxide Nanoparticles on Peritonitis in Rats Induced by Staphylococcus epidermidis Infection

Anti-Inflammatory Effects of Cerium Dioxide Nanoparticles on Peritonitis in Rats Induced by Staphylococcus epidermidis Infection

Nanotherapies for sepsis by regulating inflammatory signals and reactive oxygen and nitrogen species: New insight for treating COVID-19

Nanoplatforms for Sepsis Management: Rapid Detection/Warning, Pathogen Elimination and Restoring Immune Homeostasis

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