Cerebrospinal fluid adenosine deaminase activity for the diagnosis of tuberculous meningitis in children

Mishra, Om P.; Loiwal, V.; Ali, Ziledar; Nath, Gopal; Chandra, Lal

doi:10.1093/tropej/42.3.129

Cited by 24 publications

(21 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…ADA2 is almost exclusively identified in macrophages and is elevated in a variety of infections and diseases involving the immune system. Several studies have been performed to evaluate the utility of ADA measurements in the CSF to improve the diagnosis of TBM (21,36,40,48,62,87,116,131,132,176). The measured sensitivities and sensitivities range from 44 to 100% and 71 to 100%, respectively (Table 5).…”

Section: Molecular and Biochemical Analysismentioning

confidence: 99%

Central Nervous System Tuberculosis: Pathogenesis and Clinical Aspects

et al. 2008

View full text Add to dashboard Cite

SUMMARY Tuberculosis of the central nervous system (CNS) is a highly devastating form of tuberculosis, which, even in the setting of appropriate antitubercular therapy, leads to unacceptable levels of morbidity and mortality. Despite the development of promising molecular diagnostic techniques, diagnosis of CNS tuberculosis relies largely on microbiological methods that are insensitive, and as such, CNS tuberculosis remains a formidable diagnostic challenge. Insights into the basic neuropathogenesis of Mycobacterium tuberculosis and the development of an appropriate animal model are desperately needed. The optimal regimen and length of treatment are largely unknown, and with the rising incidence of multidrug-resistant strains of M. tuberculosis, the development of well-tolerated and effective antibiotics remains a continued need. While the most widely used vaccine in the world largely targets this manifestation of tuberculosis, the BCG vaccine has not fulfilled the promise of eliminating CNS tuberculosis. We put forth this review to highlight the current understanding of the neuropathogenesis of M. tuberculosis, to discuss certain epidemiological, clinical, diagnostic, and therapeutic aspects of CNS tuberculosis, and also to underscore the many unmet needs in this important field.

show abstract

Section: Molecular and Biochemical Analysismentioning

confidence: 99%

Central Nervous System Tuberculosis: Pathogenesis and Clinical Aspects

et al. 2008

View full text Add to dashboard Cite

show abstract

“…Adenosine deaminase (ADA) activity in the CSF of TBM patients has been suggested to be useful for early differential diagnosis of TBM [11]. The ADA activity of CSF and plasma have been evaluated as a diagnostic aid in TBM [12] and ADA activity in CSF was considered to be a simple, useful and rapid diagnostic test for early recognition of TBM in children [13]. However, overexpression of ADA was also often overexpressed in other forms of meningitis including infections with pyogenic bacteria [14].…”

Section: Introductionmentioning

confidence: 99%

Quantitative proteomics for identifying biomarkers for tuberculous meningitis

et al. 2012

View full text Add to dashboard Cite

IntroductionTuberculous meningitis is a frequent extrapulmonary disease caused by Mycobacterium tuberculosis and is associated with high mortality rates and severe neurological sequelae. In an earlier study employing DNA microarrays, we had identified genes that were differentially expressed at the transcript level in human brain tissue from cases of tuberculous meningitis. In the current study, we used a quantitative proteomics approach to discover protein biomarkers for tuberculous meningitis.MethodsTo compare brain tissues from confirmed cased of tuberculous meningitis with uninfected brain tissue, we carried out quantitative protein expression profiling using iTRAQ labeling and LC-MS/MS analysis of SCX fractionated peptides on Agilent’s accurate mass QTOF mass spectrometer.Results and conclusionsThrough this approach, we identified both known and novel differentially regulated molecules. Those described previously included signal-regulatory protein alpha (SIRPA) and protein disulfide isomerase family A, member 6 (PDIA6), which have been shown to be overexpressed at the mRNA level in tuberculous meningitis. The novel overexpressed proteins identified in our study included amphiphysin (AMPH) and neurofascin (NFASC) while ferritin light chain (FTL) was found to be downregulated in TBM. We validated amphiphysin, neurofascin and ferritin light chain using immunohistochemistry which confirmed their differential expression in tuberculous meningitis. Overall, our data provides insights into the host response in tuberculous meningitis at the molecular level in addition to providing candidate diagnostic biomarkers for tuberculous meningitis.

show abstract

“…Elevated levels in body fluids indicate activation of cellular immunity involving T cells [8]. Previous studies [9][10][11] have used adenosine deaminase levels to differentiate tuberculous from viral and bacterial meningitis as it has been found that tuberculous meningitis is associated with higher levels of this enzyme than other forms of meningitis.…”

Section: Introductionmentioning

confidence: 99%