Cerebrosides and Steroids from the Edible Mushroom Meripilus giganteus with Antioxidant Potential

Abstract

The detailed chemical analysis of the methanol extract of Meripilus giganteus (Pers.) P. Karst. led to the isolation of two new cerebrosides, mericeramides A (1) and B (2) together with cerebroside B (3), ergosterol (4), 3β-hydroxyergosta-7,22-diene (5), cerevisterol (6), 3β-hydroxyergosta-6,8(14),22-triene (7), 3β-O-glucopyranosyl-5,8-epidioxyergosta-6,22-diene (8) and (11E,13E)-9,10-dihydroxy-11,13-octadecadienoic acid (9). The structures of the compounds were determined on the basis of NMR and MS spectroscopic analysis. Mericeramide A (1) is the first representative of halogenated natural cerebrosides. The isolated fungal metabolites 1–9 were evaluated for their antioxidant activity using the oxygen radical absorbance capacity (ORAC) assay. Compounds 2, 5 and 9 proved to possess considerable antioxidant effects, with 2.50 ± 0.29, 4.94 ± 0.37 and 4.27 ± 0.05 mmol TE/g values, respectively. The result obtained gives a notable addition to the chemical and bioactivity profile of M. giganteus, highlighting the possible contribution of this species to a versatile and balanced diet.

Metabolomics Profiling of White Button, Crimini, Portabella, Lion’s Mane, Maitake, Oyster, and Shiitake Mushrooms Using Untargeted Metabolomics and Targeted Amino Acid Analysis

“…Polyporusterone E, detected in the fruiting body of Polyporus umbellatus , is reported to exhibit cytotoxic activity on leukemia 1210 cell proliferation [ 38 ]. Cerebroside B, detected in Meripilus giganteus , has possible antioxidant properties (oxygen radical absorbance capacity [ORAC] 1.69 ± 0.20 mmol TE/g) [ 39 ] and has demonstrated inhibitory effects on the proliferation of human breast cancer in MCF-7 cells [ 40 ]. Cerebroside B concentrations have been described for several edible mushroom species including king oyster (0.95 mg/g dry weight), pearl oyster (1.06 mg/g dry weight), and white button (0.59 mg/g dry weight) [ 41 ].…”

“…A considerable amount of this discussion focuses on interesting (i.e., potential mushroom-specific, bioactive, etc.) compounds detected in our mushroom samples and potential health benefits [ 33 , 37 , 38 , 39 , 40 , 42 ]. As previously mentioned, evidence to support these potential health impacts is based on isolated compounds from mushrooms and not on the consumption of whole, dietary mushrooms.…”

Is the mushroom Meripilus giganteus a potential cancer chemopreventive agent?

“…On the contrary, its pharmacological potential seems to be noteworthy. Indeed, different authors demonstrated its antioxidant and antibacterial properties (Karaman et al, 2014;Petrovi c et al, 2022;Sárközy et al, 2020). Alongside these demonstrated abilities, there are some results also in support of the cancer chemopreventive potential of M. giganteus.…”

Structure and Biological Activity of Ergostane-Type Steroids from Fungi

“…The oxygen radical antioxidant capacity (ORAC) assay of components of the edible mushroom Meripilus giganteus revealed the highest antioxidant activity (4.94 mmol TE/g) for stellasterol ( 8 ) [ 91 ].…”