Cerebral protein kinase C and its mRNA level in apolipoprotein E-deficient mice

Hung, Mei Chu; Hayase, Kaoru; Yoshida, Riki; Sato, Masao; Imaizumi, Kazunori

doi:10.1016/s0024-3205(01)01221-8

Cited by 9 publications

(6 citation statements)

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“…This would counteract the impaired neurotransmittermediated signal transduction that has been reported in apoE KO mice [8]. Indeed, reductions in the level and/or activity of GPCR and their corresponding G proteins, as well as of their down-stream effectors, such as PLC/PKC have been reported in both neurodegenerative disorders associated with apoE deficiency [9,10] and in apoE KO mice [11]. Nevertheless, the participation of additional mechanisms in these processes cannot be excluded.…”

Section: Discussionmentioning

confidence: 98%

“…Accordingly, brains from apoE KO mice show alterations in the phospholipid and fatty acid composition when compared with control mice [7], which could in part explain the impairment in neurotransmitter-associated signaling reported in these mice [8]. In this context, impaired coupling of neurotransmitter receptors (e.g., muscarinic M1 receptors) to G proteins as well as reductions in protein kinase A (PKA), phospholipase C (PLC) and protein kinase C (PKC) activities have been observed in brain tissues from both patients with AD [9,10] and apoE KO mice [11].…”

Section: Introductionmentioning

confidence: 99%

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Olive Oils Modulate Fatty Acid Content and Signaling Protein Expression in Apolipoprotein E Knockout Mice Brain

Alemany

Navarro

Vögler

et al. 2009

Lipids

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Atherosclerosis contributes to disruption of neuronal signaling pathways by producing lipid-dependent modifications of brain plasma membranes, neuroinflammation and oxidative stress. We investigated whether long-term (11 weeks) consumption of refined- (ROO) and pomace- (POO) olive oil modulated the fatty acid composition and the levels of membrane signaling proteins in the brain of apolipoprotein E (apoE) knockout (KO) mice, an animal model of atherosclerosis. Both of these oils are rich in bioactive molecules with anti-inflammatory and antioxidant effects. ROO and POO long-term consumption increased the proportion of monounsaturated fatty acids (MUFAs), particularly of oleic acid, while reducing the level of the saturated fatty acids (SFAs) palmitic and stearic acid. As a result, the MUFA:SFA ratio was higher in apoE KO mice brain fed with ROO and POO. Furthermore, both oils reduced the level of arachidonic and eicosapentaenoic acid, suggesting a decrease in the generation of pro- and anti-inflammatory eicosanoids. Finally, ROO and POO induced an increase in the density of membrane proteins implicated in both the Galphas/PKA and Galphaq/PLCbeta1/PKCalpha signaling pathways. The combined effects of long-term ROO and POO consumption on fatty acid composition and the level of signaling proteins involved in PKA and PKC activation, suggest positive effects on neuroinflammation and brain function in apoE KO mice brain, and convert these oils into promising functional foods in diseases involving apoE deficiency.

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Olive Oils Modulate Fatty Acid Content and Signaling Protein Expression in Apolipoprotein E Knockout Mice Brain

Alemany

Navarro

Vögler

et al. 2009

Lipids

View full text Add to dashboard Cite

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“…In fact, correction of cellular signals of PKC activity and MAP‐kinase in line with NF‐κB is considered to be the main action of thiazolidinedione (Straus et al. 2000; Hung et al. 2001), serving as a potent anti‐inflammatory agent (Duez et al.…”

Section: Discussionmentioning

confidence: 99%

Correction of protein kinase C activity and macrophage migration in peripheral nerve by pioglitazone, peroxisome proliferator activated‐γ‐ligand, in insulin‐deficient diabetic rats

Yamagishi

Ogasawara

Mizukami

et al. 2007

Journal of Neurochemistry

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Pioglitazone, one of thiazolidinediones, a peroxisome proliferator‐activated receptor (PPAR)‐γ ligand, is known to have beneficial effects on macrovascular complications in diabetes, but the effect on diabetic neuropathy is not well addressed. We demonstrated the expression of PPAR‐γ in Schwann cells and vascular walls in peripheral nerve and then evaluated the effect of pioglitazone treatment for 12 weeks (10 mg/kg/day, orally) on neuropathy in streptozotocin‐diabetic rats. At end, pioglitazone treatment improved nerve conduction delay in diabetic rats without affecting the expression of PPAR‐γ. Diabetic rats showed suppressed protein kinase C (PKC) activity of endoneurial membrane fraction with decreased expression of PKC‐α. These alterations were normalized in the treated group. Enhanced expression of phosphorylated extracellular signal‐regulated kinase detected in diabetic rats was inhibited by the treatment. Increased numbers of macrophages positive for ED‐1 and 8‐hydroxydeoxyguanosine‐positive Schwann cells in diabetic rats were also corrected by the treatment. Pioglitazone lowered blood lipid levels of diabetic rats, but blood glucose and nerve sorbitol levels were not affected by the treatment. In conclusion, our study showed that pioglitazone was beneficial for experimental diabetic neuropathy via correction of impaired PKC pathway and proinflammatory process, independent of polyol pathway.

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“…Apolipoprotein E has a major physiologic role in the regulation of overall lipid homeostasis and also plays an important role in neuronal repair (31)(32)(33). It has been shown that apoE is mainly synthesized by astrocytes within which it is packaged with cholesterol and phospholipid to form lipid/protein complexes, which are then released into the extracellular space (34).…”

Section: Discussionmentioning

confidence: 99%

APOE4 Protects the Cognitive Development in Children with Heavy Diarrhea Burdens in Northeast Brazil

et al. 2005

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Polymorphisms in the apolipoprotein E (APOE) have constituted the major rationale to identify potential risk groups for developing late-onset Alzheimer's disease and help to predict recovery of cognitive function after brain injury. However, the APOE impact on cognitive development in children living in poor areas of the developing world, where we have discovered profound significant associations of early childhood diarrhea (at 0-2 y) with lasting impairments of growth, cognition, and school performance, is not known. Therefore, we conducted APOE genotyping in 72 Brazilian shantytown children under active surveillance since birth, using purified DNA extracted from buccal cell samples. We found a high frequency of APOE4 alleles (18% versus 9-11% expected) in children with lower diarrhea burdens. When we examined the children who experienced the heavier diarrhea burdens (greater than or equal to the median of seven illnesses in the first 2 y of life), those with APOE4 did significantly better in the coding subtest (p=0.01) when compared with APOE4-negative children with similar diarrhea burdens. Positive correlations between the APOE4 occurrence and coding scores remained, even after adjusting for family income, maternal education, and breast-feeding. Moreover, the APOE4-positive group, under heavy burdens of diarrhea, had preserved semantic fluency and the mean difference in fluency scores, p=0.025, a standardized coefficient for disproportional verbal fluency impairment. Our findings show that APOE4 is relatively common in favela children and suggest a protective role of the APOE4 allele in children with a history of heavy burdens of diarrhea in their first 2 y of life.

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Cerebral protein kinase C and its mRNA level in apolipoprotein E-deficient mice

Cited by 9 publications

References 0 publications

Olive Oils Modulate Fatty Acid Content and Signaling Protein Expression in Apolipoprotein E Knockout Mice Brain

Olive Oils Modulate Fatty Acid Content and Signaling Protein Expression in Apolipoprotein E Knockout Mice Brain

Correction of protein kinase C activity and macrophage migration in peripheral nerve by pioglitazone, peroxisome proliferator activated‐γ‐ligand, in insulin‐deficient diabetic rats

APOE4 Protects the Cognitive Development in Children with Heavy Diarrhea Burdens in Northeast Brazil

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