Cerebral plasminogen activator activity in spontaneously hypertensive stroke-prone rats.

Abstract

Background and Purpose:In the spontaneously hypertensive stroke-prone rat, it is unclear whether plasminogen activator plays a role in the development of stroke. The present study was undertaken to investigate brain levels of plasminogen activator activity in spontaneously hypertensive stroke-prone rats and Wistar-Kyoto rats.Methods: Plasminogen activator was purified from the brains of rats of both strains. The purification involved used ammonium sulfate precipitation, gel filtration, a zinc chelate-Sepharose column, and a concanavalin A-Sepharose column. Fraction I (0.15 M KCl-soluble fraction) and fraction II (2 M KCI plus 6 M urea-soluble fraction) were purified from both strains.Results: Total plasminogen activator activity in the original homogenates for fractions I and II derived from spontaneously hypertensive stroke-prone rats was increased to twice the level found in Wistar-Kyoto rats. The final product purified from fractions I and II in both strains of rats revealed single bands of plasminogen activator activity on enzymatic analysis with a molecular weight of 72,000. The purified product had stronger S-2288 amidolytic activity than S-2444 amidolytic activity, and it also displayed fibrin-binding ability.Conclusions: The study demonstrated that there is an increased content of plasminogen activator in the brains of spontaneously hypertensive stroke-prone rats that might be related to the development of stroke.

Is the Spontaneously Hypertensive Stroke Prone Rat a Pertinent Model of Sub Cortical Ischemic Stroke? A Systematic Review

“…MMPs and plasmin system potentially cooperate in matrix degradation, tissue damage & /remodelling. Note all studies concerning inflammatory markers were conducted in the presence of salt loading or an unknown diet initiated at six-weeks of age (22,(78)(79)(80)(81)(82)(83)(84)(85). NO, nitric oxide; PA, plasminogen activator; MMP, matrix metalloproteinase; SHRSP, spontaneously hypertensive stroke prone rat; WKY, Wistar Kyoto rats; MRI, magnetic resonance imaging.…”

“…SHRSP grew faster (93), but produced less than half the amount of tissue plasminogen activator as age-matched WKY rats (82). Unfortunately, this has not been investigated in cerebral vessels.…”

Fibrinolytic Activity in Liver Tissues of Stroke‐prone Spontaneously Hypertensive Rats

“…The molecular weight of the plasminogen activator was 67 kDa, 44 kDa and 38 kDa in fraction I, and 67 kDa and 44 kDa in fraction 11, respectively. In the previous studies, the plasminogen activator in both rats was t-PA with the molecular weight of 72 kDa in brain tissue (Matsuo et al 1992), and was u-PA with the molecular weight of 43 kDa, and 37 kDa and t-PA with the molecular weight of 67 kDa in kidney tissue (Okada et al 1990). Furthermore, the liver tissues of Wistar rats were analysed for plasminogen activator activity and t-PA mRNA (Quax et al 1990).…”

“…The total plasminogen activator activity which was calculated as the sum of fractions I and I1 was about 1.5-fold higher in WKY than in SHRSP. By contrast, the plasminogen activator activity in the brain (Matsuo et al, 1992) and kidney (Okada et al 1990) tissues of SHRSP has been reported to be about 1.5-2.0-fold higher than that of WKY. Thus, the content of fibrinolytic activity differs with the tissue in WKY and SHRSP.…”

“…1974). The plasminogen activator activity in the brain tissue of SHRSP has previously been reported to be about 2.0 fold higher than that of Wistar-Kyoto (WKY) rats, the strain from which SHRSP were derived (Matsuo et al 1992). Furthermore, the plasminogen activator purified from the brain tissue of both rats was identified as t-PA with a molecular weight of 72 kDa.…”