Cerebral Microvascular Endothelial Cell Apoptosis after Ischemia: Role of Enolase-Phosphatase 1 Activation and Aci-Reductone Dioxygenase 1 Translocation

Zhang, Yuan; Wang, Ting; Yang, Ke; Xu, Jiang; Ren, Lijie; Li, Weiping; Liu, Wenlan

doi:10.3389/fnmol.2016.00079

Cited by 26 publications

(26 citation statements)

References 31 publications

(45 reference statements)

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“…5C). Several of the proteins or their mammalian homologs, including GAR1 (probable H/ACA ribonucleoprotein complex subunit), GSTT3 (glutathione S-transferase D3), PSA5 (proteasome subunit a type-5), TMEDE (transmembrane emp24 domain-containing protein eca), REF2P [protein ref (2)P], CP391 (probable CYP309a1), and ENOPH (enolase-phosphatase E1), that were identified in the Furin1-knockdown fat bodies have been associated with stress responses (52)(53)(54)(55)(56). For example, the human homolog for TMEDE/eca (i.e., TMED4 or ERS25) is induced by endoplasmic reticulum-specific stress, heat shock, and oxidative stress (54).…”

Section: Comparison Of the Morphology Of The Fat Body And Proteomes Omentioning

confidence: 99%

Proprotein convertaseFurin1expression in theDrosophilafat body is essential for a normal antimicrobial peptide response and bacterial host defense

et al. 2017

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Invading pathogens provoke robust innate immune responses in Dipteran insects, such as In a systemic bacterial infection, a humoral response is induced in the fat body. Gram-positive bacteria trigger the Toll signaling pathway, whereas gram-negative bacterial infections are signaled the immune deficiency (IMD) pathway. We show here that the RNA interference-mediated silencing of -a member of the proprotein convertase enzyme family-specifically in the fat body, results in a reduction in the expression of antimicrobial peptides. This, in turn, compromises the survival of adult fruit flies in systemic infections that are caused by both gram-positive and -negative bacteria. plays a nonredundant role in the regulation of immune responses, as silencing of , the other member of the enzyme family, had no effect on survival or the expression of antimicrobial peptides upon a systemic infection. does not directly affect the Toll or IMD signaling pathways, but the reduced expression of up-regulates stress response factors in the fat body. We also demonstrate that is a negative regulator of the Janus kinase/signal transducer and activator of transcription signaling pathway, which is implicated in stress responses in the fly. In summary, our data identify as a novel regulator of humoral immunity and cellular stress responses in-Aittomäki, S., Valanne, S., Lehtinen, T., Matikainen, S., Nyman, T. A., Rämet, M., Pesu, M. Proprotein convertase expression in the fat body is essential for a normal antimicrobial peptide response and bacterial host defense.

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Section: Comparison Of the Morphology Of The Fat Body And Proteomes Omentioning

confidence: 99%

Proprotein convertaseFurin1expression in theDrosophilafat body is essential for a normal antimicrobial peptide response and bacterial host defense

et al. 2017

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“…ENOPH1, as the methionine salvage pathway enzyme, has been revealed to play a role in stress reactivity (10) and ischemic blood-brain barrier (BBB) dysfunction (21). However, its possible effects on glioma progression and potential mechanisms have not been fully elucidated.…”

Section: Discussionmentioning

confidence: 99%

“…Co., Ltd., Fuzhou, China), the coverslips were mounted on glass slides, immunostaining was imaged with the use of a DMI6000B fluorescence microscope (Leica Microsystems GmbH, Wetzlar, Germany). The average fluorescence intensity of ENOPH1 in the cells in the image was determined as previously described (21).…”

Section: Usa) Was Added To Each Well and Cultured For 4 H At 37˚cmentioning

confidence: 99%

Enolase-phosphatase 1 as a novel potential malignant glioma indicator promotes cell proliferation and migration

Yang

et al. 2018

Oncol Rep

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Enolase-phosphatase 1 (ENOPH1), is an enzyme that is involved in polyamine biosynthesis and is associated with stress responses. However, little is known about its role in the pathophysiology of glioma. In the present study, we examined the expression and function of ENOPH1 in human glioma tissues and cell lines. Western blot, qPCR and immunohistochemistry analysis were performed to investigate the expression of the ENOPH1 protein in glioma tissues in 86 patients. The 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT), wound healing and cell cycle assays were implemented to identify cell growth and cell migration in U87 and U251 glioma cells. The results revealed that compared with normal brain tissues, the level of ENOPH1 was markedly increased in glioma tissues. In addition, we observed that the glioma pathological grade was positively associated with the expression level of ENOPH1. Knockdown of ENOPH1 expression with siRNA markedly reduced cell proliferation, and significantly decreased cell migration. Notably, knockdown of ENOPH1 promoted its downstream protein, aci-reductone dioxygenase 1 (ADI1), to shift from the nucleus to the cytoplasm of U251 glioma cells, while MT1-MMP expression was significantly downregulated compared with the control group. Collectively, our data demonstrated that the knockdown of ENOPH1 suppressed cell growth and migration, which may be associated with ADI1 translocation and MT1-MMP downregulation in glioma cells. Thus, ENOPH1 could serve as an underlying therapeutic target of glioma.

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“…This enzyme is released during methionine metabolism and regulates the stress response of cells. Overexpression or complete blockage of ENOPH1 resulted in increased cell mortality [48].…”

Section: Discussionmentioning

confidence: 99%

Amino acids metabolism and degradation is regulated during porcine oviductal epithelial cells (OECs) primary culture in vitro – a signaling pathways activation approach

Kranc

Jankowski

Budna

et al. 2018

Medical Journal of Cell Biology

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The ovary is part of the reproductive system, possessing very important functions in the reproduction process (ovum and embryo transfer, providing a suitable environment for sperm capacitation, etc.). There are two types of cells in the fallopian tubes: alveolar and secretive cells. These study shows the metabolic processes in pig oviductal epithelial cells associated with the activation of signaling pathways of amino acids metabolism and degradation during long-term in vitro culture. Oviductal epithelial cells from 45 colonies in the anestrous phase of the estrous cycle have been utilized in this study. RNA extract from the OEC primary cultures was pooled after 24h, 7days, 15 days and 30 days from the beginning of culture and the transcriptome investigated by Affymetrix® Porcine Gene 1.1 ST. From the whole transcript that consisted of 2009 different genes, 1537 were upregulated and 995 were downregulated after 7 days of culture, 1471 were upregulated and 1061 were downregulated after 15 days of culture and 1329 were upregulated and 1203 were downregulated after 30 days of culture. The results of these studies provide, for the first time, information on the activation of metabolic pathways of amino acids such as valine, leucine, isoleucine, cysteine, and methionine in the investigated tissue. They also indicate genes that may be OECs-specific genetic markers that are expressed or upregulated during long-term in vitro culture.Running title: regulation of amino acid signaling pathways

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Cerebral Microvascular Endothelial Cell Apoptosis after Ischemia: Role of Enolase-Phosphatase 1 Activation and Aci-Reductone Dioxygenase 1 Translocation

Cited by 26 publications

References 31 publications

Proprotein convertaseFurin1expression in theDrosophilafat body is essential for a normal antimicrobial peptide response and bacterial host defense

Proprotein convertaseFurin1expression in theDrosophilafat body is essential for a normal antimicrobial peptide response and bacterial host defense

Enolase-phosphatase 1 as a novel potential malignant glioma indicator promotes cell proliferation and migration

Amino acids metabolism and degradation is regulated during porcine oviductal epithelial cells (OECs) primary culture in vitro – a signaling pathways activation approach

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