Cerebral columnar organization of the first nociceptive component induced by CO2 laser on the tail of the rat

Jaw, Fu‐Shan; Kao, Yu-Chieh Jill; Chen, Chih‐Ping; Liao, Wei-Hao

doi:10.1016/j.neuroscience.2008.09.046

Cited by 5 publications

(6 citation statements)

References 32 publications

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“…Identities of information processing such ceptive neurons are primarily located across layers II-VI, while nociceptive cells are mostly located in layers V-VI, suggesting a layer-related difference in functional populations of neurons in S1 area [9,10] . Moreover, similar results have been reported recently, by selective activation of peripheral Aδ fibers using CO 2 laser stimulation to the rat tail [4] . However, little has been reported concerning the layer-related difference in spatial and temporal characteristics of local neural circuits in the hindlimb representation of the rat S1 area either in vivo or in vitro, although the hind paw withdrawal reflex is mostly used as a quantitative index for nociceptive responses in both behavioral and electrophysiological recordings.…”

Section: Introductionsupporting

confidence: 89%

“…From the viewpoint of cytoarchitectonics and anatomy, the neocortical structure is featured by its laminar (layer) distribution parallel to the cortical surface [3,4] . In addition, it has been proposed that the neocortex works in a unique columnar organization, namely the functional and structural unit, to represent peripheral receptive fields of the body [3] .…”

Section: Introductionmentioning

confidence: 99%

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Neural circuits and temporal plasticity in hindlimb representation of rat primary somatosensory cortex: revisited by multi-electrode array on brain slices

Wang

Chang

et al. 2010

Neurosci. Bull.

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Objective The well-established planar multi-electrode array recording technique was used to investigate neural circuits and temporal plasticity in the hindlimb representation of the rat primary somatosensory cortex (S1 area).Methods Freshly dissociated acute brain slices of rats were subject to constant perfusion with oxygenated artificial cerebrospinal fluid (95% O 2 and 5% CO 2 ), and were mounted on a Med64 probe (64 electrodes, 8×8 array) for simultaneous multi-site electrophysiological recordings. Current sources and sinks across all the 64 electrodes were transformed into twodimensional current source density images by bilinear interpolation at each point of the 64 electrodes. Results The local intracortical connection, which is involved in mediation of downward information flow across layers II-VI, was identified by electrical stimulation (ES) at layers II-III. The thalamocortical connection, which is mainly involved in mediation of upward information flow across layers II-IV, was also characterized by ES at layer IV. The thalamocortical afferent projections were likely to make more synaptic contacts with S1 neurons than the intracortical connections did. Moreover, the S1 area was shown to be more easily activated and more intensively innervated by the thalamocortical afferent projections than by the intracortical connections. Finally, bursting conditioning stimulus (CS) applied within layer IV of the S1 area could successfully induce long-term potentiation (LTP) in 5 of the 6 slices (83.3%), while the same CS application at layers II-III induced no LTP in any of the 6 tested slices. Conclusion The rat hindlimb representation of S1 area is likely to have at least 2 patterns of neural circuits on brain slices: one is the intracortical circuit (ICC) formed by interlaminar connections from layers II-III, and the other is the thalamocortical circuit (TCC) mediated by afferent connections from layer IV. Besides, ICC of the S1 area is spatially limited, with less plasticity, while TCC is spatially extensive and exhibits a better plasticity in response to somatosensory afferent stimulation. The present data provide a useful experimental model for further studying microcircuit properties in S1 cortex at the network level in vitro.

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Section: Introductionsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Neural circuits and temporal plasticity in hindlimb representation of rat primary somatosensory cortex: revisited by multi-electrode array on brain slices

Wang

Chang

et al. 2010

Neurosci. Bull.

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“…This conclusion has wide implications, not only retrospectively, as it prompts a reconsideration of previous interpretations based on the so-called “Aδ-ERPs” in basic, preclinical, and pharmacological research, 46 , 52 , 57 but also prospectively, as laser stimulation is considered one of the best tools to investigate pain psychophysics in rats and humans, and is therefore being increasingly used. 5 , 7 , 11 – 13 , 22 , 24 – 26 , 28 , 35 , 36 , 45 , 46 , 48 , 50 , 52 , 55 , 57 , 59 These results have implications in the interpretation of results obtained with other nociceptive assays. For example, the Hargreaves test, which also uses radiant heat, is likely to mostly reflect the function of C-fibres.…”

Section: Discussionmentioning

confidence: 88%

“…This is relevant given the growing number of studies using laser nociceptive stimulation in rat assays of nociception and pain. 5 , 11 , 22 , 26 , 35 , 36 , 50 , 59 …”

Section: Discussionmentioning

confidence: 99%

Was it a pain or a sound? Across-species variability in sensory sensitivity

Xia

Peng

et al. 2015

Pain

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“…Bregma was used as the origin, and the coordinates for recording were derived from the Atlas of the Rat Brain [ 7 ]. Extracellular action potentials (APs) were recorded in the tail representation area of the primary somatosensory cortex (1.8 to 1.9 mm lateral to the midline, 1.8 to 1.9 mm caudal to bregma, depth of 300 to 1500 μm from the brain surface) [ 8 ]. The rectal temperature of the rats was kept at 37°C ± 0.5°C with a homeothermic blanket.…”

Section: Methodsmentioning

confidence: 99%

Quantitative pinch stimulator for exploring evoked nociceptive responses: A pilot study

et al. 2010

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BackgroundA mechanical noxious stimulator is useful for studies of pain, both for clinic and basic research. We propose to use a pinch stimulator that can not only generate a quantitative, reproducible noxious pinch but also simultaneously provide a synchronous external trigger signal, which is essential for acquisition of evoked potentials.MethodsFor ethical considerations, audible and visual aids were incorporated so that pinch force could be regulated within a predetermined level. Reproducibility of the nociceptive responses evoked by this device was validated. The device was constructed with a simple circuit, and the element build-in was delicately selected for the minimum required to produce evoked potentials.ResultsThe magnitude of the force output is linearly proportional to the volts produced by the device (i.e., during the pinch). Increases in force correspond to increases in the number of action potentials induced.ConclusionsThis device may be useful for studying the mechanisms of nociceptive signal processing in the brain through application of reproducible, noxious pinch stimuli.

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Cerebral columnar organization of the first nociceptive component induced by CO2 laser on the tail of the rat

Cited by 5 publications

References 32 publications

Neural circuits and temporal plasticity in hindlimb representation of rat primary somatosensory cortex: revisited by multi-electrode array on brain slices

Neural circuits and temporal plasticity in hindlimb representation of rat primary somatosensory cortex: revisited by multi-electrode array on brain slices

Was it a pain or a sound? Across-species variability in sensory sensitivity

Quantitative pinch stimulator for exploring evoked nociceptive responses: A pilot study

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