Cerebral blood flow and morphological changes after hypoxic-ischaemic injury in preterm lambs

Hilario, Enrique; Rey-Santano, Mari Carmen; Goñi‐de‐Cerio, Felipe; Álvarez, Francisco J.; Gastiasoro, Elena; Mielgo, Victoria; Caballero, A; Valls-i-Soler, Adolfo; Gómez-Urquijo, Sonia M; Álvarez, Antonia

doi:10.1080/08035250510031151

Cited by 28 publications

(25 citation statements)

References 25 publications

(29 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This was associated with significant reduction of excitatory amino acids level in the striatum suggestive of more favorable neurologic outcome in Re-O 2 HI-piglets (5). In preterm lambs subjected to HI-insult the severity of cerebral regional hypoperfusion strongly correlated with increased apoptotic cell death-rate (21). In our study, improved late neurologic outcome in Re-O 2 mice compared with Re-Air mice may be secondary to the more efficient restoration of hypoxia-depressed CBF during Re-O 2 .…”

supporting

confidence: 49%

Reoxygenation with 100% Oxygen Versus Room Air: Late Neuroanatomical and Neurofunctional Outcome in Neonatal Mice with Hypoxic-Ischemic Brain Injury

et al. 2006

View full text Add to dashboard Cite

Study investigated neuroutcome in mice subjected at 7-8 d of life to hypoxic-ischemic brain injury (HI) followed by 30 min of reoxygenation with 100% O 2 (Re-O 2 ) or room air (Re-Air). At 24 h of recovery, mouse reflexes were tested. At 7 wks after HI spatial orientation and memory were assessed in the same mice. Mortality rate was recorded at 24 h and at 7 wks of recovery. In separate cohort of mice, changes in cerebral blood flow (CBF) during HI-insult and reoxygenation were recorded. T he resuscitation of asphyxiated neonates is aimed to reestablish tissue oxygen delivery by restoring blood flow and increasing the arterial oxygen content. Use of 100% O 2 during neonatal resuscitation is currently recommended (1). However, Re-O 2 compared with Re-Air significantly exacerbates an oxidative stress in asphyxiated neonates (2), which is one of the mechanisms of HI-brain injury (3). To avoid hyperoxia-induced oxidative stress, the efficacy of resuscitation with room air (RA) instead of 100% O 2 is being extensively studied. To date, results of these studies are conflicting. For instance, it was reported that in asphyxiated pigs the Re-O 2 restored CBF significantly faster and more complete compared with Re-Air (4). Significantly higher levels of excitatory amino acids were found in the striatum of newborn HI-piglets Re-Air compared with Re-O 2 piglets which may be indicative of less favorable neurologic prognosis for Re-Air animals (5). However, Liu. Y and co-authors, using a canine model of cardiac arrest, demonstrated that Re-O 2 versus ReAir resulted in greater neurologic impairment assessed at 24 h of recovery (6). In contrast, the postischemic use of hyperbaric oxygenation, which produces substantial oxidative stress (7), significantly improved neurologic score following focal cerebral ischemia in adult rats and HI in neonatal rats (8,9). Hyperbaric oxygenation used as a mode of resuscitation from experimental heatstroke in rats was shown to be significantly neuroprotective (10). Meier and co-authors reported that hyperoxic ventilation significantly reduced short-term (6 h) mortality due to systemic ischemia following an acute hemorrhagic shock in pigs (11). The most recent meta-analysis indicates that there are not enough data to challenge current recommendation of using of pure O 2 during resuscitation (12).The assessment of long-term neurologic handicap following HI is of paramount clinical value to define the ultimate efficacy of Re-O2 versus Re-Air in an asphyxiated subject. There are no reports on late neurologic outcome and reoxygenation strategy following asphyxia. This work was undertaken to provide experimental data on short-and long-term neuropathological and neurofunctional outcomes in HIaffected mice re-oxygenated with either 100% O 2 or RA immediately following the index event. MATERIALS AND METHODSMurine model of HI. Three day old (p3) C57/BL6J mice of both genders were purchased from Jackson Laboratories (Bar Harbor, ME) with their birth mothers. All research was conducted according ...

show abstract

supporting

confidence: 49%

Reoxygenation with 100% Oxygen Versus Room Air: Late Neuroanatomical and Neurofunctional Outcome in Neonatal Mice with Hypoxic-Ischemic Brain Injury

et al. 2006

View full text Add to dashboard Cite

show abstract

“…A significant proportion of fetuses had ACA PI values below the 5th percentile, despite presenting an MCA PI within the normal range and hence not showing cerebral blood flow redistribution as currently defined. Differences in the response to hypoxia of different fetal cerebral vascular territories are also supported by experimental research in which the brain of fetal lambs showed an internal redistribution of the blood flow in relation to the degree of the hypoxic insult [3] .…”

Section: Introductionmentioning

confidence: 77%

Reference Values for Doppler Parameters of the Fetal Anterior Cerebral Artery throughout Gestation

Benavides‐Serralde

Hernández‐Andrade

Figueroa‐Diesel

et al. 2009

Gynecol Obstet Invest

View full text Add to dashboard Cite

Aim: To construct normal reference values for Doppler parameters in 2 anatomical segments of the fetal anterior cerebral artery (ACA) throughout pregnancy. Methods: The ACA was evaluated in 373 normally growing fetuses from 20 to 40 weeks of gestation. The first segment of the ACA (ACA-S1) was recorded just after its origin from the internal carotid artery in the same plane as the middle cerebral artery (MCA). The second segment (ACA-S2) was recorded distal to the outlet of the anterior communicating artery. Results: The ACA pulsatility index (PI) behaved similarly in both segments, with a constant increase until 28 weeks followed by a decrease until the end of pregnancy [ACA-S1 PI = 3.49 – 0.37 × gestational age (GA) – (0.0063 × GA²), SD = 0.6 – 0.061 × GA – (0.001 × GA²); ACA-S2 PI = 1.54 – 0.22 × GA – (0.0037 × GA²), SD = 0.206 + (0.0037 × GA)]. Peak systolic velocities in both segments showed a constant increase from 20 to 40 weeks of gestation. No significant differences were found between the 2 segments with regard to any Doppler parameter. However, the angle of insonation and the time spent on examination were significantly lower and reproducibility was better for ACA-S1. Conclusion: Despite showing similar Doppler values, ACA-S1 has a higher reliability than ACA-S2 and can be recorded in the same anatomical projection as the MCA.

show abstract

“…Experimental and human studies coincide in the regionalization of vascular redistribution showing increased brain perfusion in frontal lobe, basal ganglia and cerebellum [45,46]. The preferential involvement of the frontal lobe in fetal growth restriction has been reported by different authors.…”

Section: Discussionmentioning

confidence: 99%

Fetal Brain MRI Texture Analysis Identifies Different Microstructural Patterns in Adequate and Small for Gestational Age Fetuses at Term

et al. 2013

View full text Add to dashboard Cite

Objectives: We tested the hypothesis whether a texture analysis (TA) algorithm applied to MRI brain images identified different patterns in small for gestational age (SGA) fetuses as compared with adequate for gestational age (AGA). Study Design: MRI was performed on 83 SGA and 70 AGA at 37 weeks' GA. Texture features were quantified in the frontal lobe, basal ganglia, mesencephalon, cerebellum and cingulum. A classification algorithm based on discriminative models was used to correlate texture features with clinical diagnosis. Results: Region of interest delineation in all areas was achieved in 61 SGA (12 vasodilated) and 52 AGA; this was the sample for TA feature extraction which allowed classifying SGA from AGA withaccuracies ranging from 90.9 to 98.9% in SGA versus AGA comparison and from 93.6 to 100% in vasodilated SGA versus AGA comparison. Conclusions: This study demonstrates that TA can detect brain differences in SGA fetuses. This supports the existence of brain microstructural changes in SGA fetuses.

show abstract

Cerebral blood flow and morphological changes after hypoxic-ischaemic injury in preterm lambs

Abstract: Our results suggest that regional cerebral blood flow and the presence of apoptotic cells, 3 h after hypoxic-ischemic injury, are correlated.

Cited by 28 publications

References 25 publications

Reoxygenation with 100% Oxygen Versus Room Air: Late Neuroanatomical and Neurofunctional Outcome in Neonatal Mice with Hypoxic-Ischemic Brain Injury

Reoxygenation with 100% Oxygen Versus Room Air: Late Neuroanatomical and Neurofunctional Outcome in Neonatal Mice with Hypoxic-Ischemic Brain Injury

Reference Values for Doppler Parameters of the Fetal Anterior Cerebral Artery throughout Gestation

Fetal Brain MRI Texture Analysis Identifies Different Microstructural Patterns in Adequate and Small for Gestational Age Fetuses at Term

Contact Info

Product

Resources

About