Ceratonia siliqua (Carob) extract improved in vitro development of vitrified-warmed mouse germinal vesicle oocytes: assessment of possible mechanism

Faramarzi, Azita; Aghaz, Farank; Bakhtiari, Mitra; Roshankhah, Shiva; Rashidi, Zahra; Khazaei, Mozafar

doi:10.1007/s10561-020-09873-w

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“…According to previous studies, the complementation of freezing-thawing environments with various factors, including antioxidants, is an effective approach to improve the quality of frozen-thawed sperm [ 13 ]. Many studies have focused on plants with antioxidant capacities that can counteract the damaging effects of free radicals due to oxidative stress [ 14 ]. Ceratonia siliqua is an evergreen plant native to the Mediterranean region.…”

Section: Introductionmentioning

confidence: 99%

Human sperm parameter improvement associated with Ceratonia siliqua extract as a cryopreservation supplement after vitrification

Farazmand

Mansouri

Koohestanidehaghi

et al. 2023

Clin Exp Reprod Med

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Objective: Given the destructive effects of oxidative stress on sperm structure, this study was conducted to investigate the antioxidant effects of different concentrations of Ceratonia siliqua plant extract on human sperm parameters after the freezing-thawing process.Methods: A total of 20 normozoospermic samples were frozen. Each sample was divided into two control groups (fresh and cryopreservation) and three cryopreservation experimental groups (containing C. siliqua extract at concentrations of 20, 30, and 40 µg/mL in the freezing extender). Motility, intracellular levels of reactive oxygen species (ROS), plasma membrane integrity (PMI), mitochondrial membrane potential (MMP), viability, and acrosome reaction parameters were evaluated. Results: Statistical analysis showed that the highest motility, viability, and PMI were associated with the 20 µg/mL concentration of C. siliqua extract. At all concentrations, intracellular ROS levels were significantly lower and the levels of MMP and the acrosome reaction were significantly higher than in the cryopreservation control group (p≤0.05).Conclusion: C. siliqua extract supplements at concentrations of 20, 30, and 40 µg/mL improved sperm motility, viability, PMI, MMP, intracellular ROS, and the acrosome reaction.

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Section: Introductionmentioning

confidence: 99%