Ceratocystiopsis spp. associated with pine- and spruce-infesting bark beetles in Norway

Jankowiak, Robert; Solheim, Halvor; Bilański, Piotr; Mukhopadhyay, Jigeesha; Hausner, Georg

doi:10.1007/s11557-022-01808-x

Cited by 4 publications

(1 citation statement)

References 44 publications

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“…To demonstrate this, we have chosen the Ophiostomatales ( Ascomycota : Sordariomycetes ) as a model, as this group of fungi are of economic importance and pose an ever‐growing threat to global biosecurity (Wingfield et al, 2017). The Ophiostomatales exhibit many of the key challenges to fungal species identification, including extensive taxonomic revisions (de Beer et al, 2021) and cryptic speciation (Jankowiak et al, 2017, 2022), as well as inconsistency in sequence data availability (Hulcr et al, 2020). These challenges have plagued previous metabarcoding studies of the Ophiostomatales , highlighting a need to assess alternative barcodes outside the rDNA operon (Hulcr et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Modular, multi‐barcode amplicon sequencing for improved species‐level detection of fungal phytopathogens: A case study of pipeline establishment targeting the Ophiostomatales

et al. 2022

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Recent advances in the field of environmental DNA (eDNA) metabarcoding have produced a promising platform for early detection and broad‐spectrum monitoring of plant pests and pathogens. To date, the majority of fungal metabarcoding assays have relied upon universal primers amplifying segments of the ribosomal DNA, to ensure broad taxonomic coverage while studying changes in community composition, or more recently, to screen for presence/absence of target species. In a diagnostics framework however, single, universal molecular barcodes do not discriminate accurately enough for many groups of important fungal phytopathogens at the required species level. Here, a modular, multi‐barcode amplicon sequencing pipeline was established to provide rapid and reliable diagnostics targeting the Ophiostomatales, an order containing economically important phytopathogens vectored by bark and ambrosia beetles (Curculionidae). Using compositionally varied mock communities, we evaluated five barcoding loci: ITS1, ITS2, the large ribosomal sub‐unit (LSU), translation elongation factor 1‐alpha (TEF1α) and calmodulin (CAL), for their ability to provide species‐level resolution. The sensitivity and detection limit of the assay was established by spiking genomic DNA of the exotic Dutch elm disease pathogen, Ophiostoma novo‐ulmi Brasier, into both the controlled mock communities and eDNA sampled from Ips grandicollis (Eichhoff) beetles collected in New South Wales, Australia. The TEF1α barcode successfully detected the Dutch elm pathogen DNA with 100% accuracy down to approx. 5 pg/μl, while the ITS1 barcode had a 100% accuracy down to approx. 0.4 pg/μl within a given sample. Our results demonstrate that a dual‐barcode approach targeting the ITS1 and TEF1α regions simultaneously is sufficient for accurate species level detection and characterisation of the Ophiostomatales and offers confident detection of low‐abundance taxa (relative read abundances of 0.1%–0.01%). Multi‐barcode metabarcoding provides a framework which can quickly and efficiently be adapted to new targets when establishing high throughput diagnostics for post‐border biosecurity surveillance of fungal phytopathogens.

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Section: Introductionmentioning

confidence: 99%

Modular, multi‐barcode amplicon sequencing for improved species‐level detection of fungal phytopathogens: A case study of pipeline establishment targeting the Ophiostomatales

et al. 2022

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The pine bark beetle Ips acuminatus: an ecological perspective on life-history traits promoting outbreaks

Papek,

Ritzer,

Biedermann

et al. 2024

J Pest Sci

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The bark beetle Ips acuminatus is an important pest in pine-dominated forests of Eurasia. Recently, the frequency of I. acuminatus outbreaks and mortality of host trees have increased, most likely as a result of climate change-related alterations in environmental conditions. Therefore, detailed information on the species’ natural history is essential to understand its potential to damage forests and to apply sustainable management measures. We provide a comprehensive overview on the life history of I. acuminatus, focusing on traits that might explain outbreaks and the ability to cause tree mortality. We review its importance for European forestry, outbreak behavior, host plant usage, reproductive biology, temperature-dependent development, diapause and overwintering behavior, and interactions with fungi, bacteria, nematodes and other arthropods. Interestingly, I. acuminatus has a strong nutritional dependency on the fungus Ophiostoma macrosporum, underlined by the presence of a prominent oral mycetangium, a spore-carrying organ, in females, which is not known for other Ips species. Moreover, I. acuminatus can reproduce sexually and asexually (pseudogamy). Additionally, information on the species’ evolutionary past provides valuable insights into the origin of certain traits. We present a phylogeny of the genus Ips and examine selected life-history traits in an evolutionary context. Together with its sister species Ips chinensis, I. acuminatus forms a separate clade within Ips. The ancestor of Ips bark beetles originated about 20 million years ago and was a pine-colonizing species inhabiting the Holarctic. Finally, open fields of research are identified to guide future work on this ecologically and economically important pine bark beetle.

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The compact mitogenome of Ceratocystiopsis pallidobrunnea

Wai

Hausner

2022

Can. J. Microbiol.

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Ceratocystiopsis is a fungal genus that has been assigned to the Ophiostomatales, fungi known for their association with various bark beetles and other arthropods. The mitochondrial genome of Ceratocystiopsis pallidobrunnea has been characterized and compared with other members of the genus Ceratocystiopsis and Ophiostomatales. At 29 022 bp, the mitogenome of C. pallidobrunnea is the smallest reported so far for this genus. Gene arrangement was observed to be conserved for this group of fungi and mitogenome variation appears to be mostly due to the absence and presence of introns. The long-term goal is to apply mitogenomes to resolve taxonomic issues within the Ophiostomatales and within the various genera that comprise the Ophiostomataceae.

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Ceratocystiopsis spp. associated with pine- and spruce-infesting bark beetles in Norway

Cited by 4 publications

References 44 publications

Modular, multi‐barcode amplicon sequencing for improved species‐level detection of fungal phytopathogens: A case study of pipeline establishment targeting the Ophiostomatales

Modular, multi‐barcode amplicon sequencing for improved species‐level detection of fungal phytopathogens: A case study of pipeline establishment targeting the Ophiostomatales

The pine bark beetle Ips acuminatus: an ecological perspective on life-history traits promoting outbreaks

The compact mitogenome of Ceratocystiopsis pallidobrunnea

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