Ceratite bilateral por Acanthamoeba: relato de caso

Obeid, Wilson Nahmatallah; Araújo, Rogério de; Vieira, Luiz Henrique Palucci; Machado, Marco Antônio Cardoso

doi:10.1590/s0004-27492003000700025

Cited by 9 publications

(8 citation statements)

References 37 publications

(40 reference statements)

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“…The increasing number of contact-lens users enhances the frequency of the illness because the trauma and hypoxia of the corneal epithelium facilitate the invasion of the parasite into the stroma. Furthermore, inadequate asepsis leads to contamination by bacteria and fungi, producing a favorable culture medium for the growth of the protozoan (Obeid et al 2003).…”

Section: Introductionmentioning

confidence: 99%

In vitro evaluation of the amebicidal activity of Pterocaulon polystachyum (Asteraceae) against trophozoites of Acanthamoeba castellanii

et al. 2008

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The crude extract and hexane, dichloromethane, and methanol fractions obtained from the aerial parts of Pterocaulon polystachyum (Asteraceae) were assayed against Acanthamoeba castellanii, a free-living ameba that causes acute amebic keratitis. Because of its capacity to form cysts, some strains of this protozoan are excellent opportunists and therapy resistant, necessitating a search for new drugs in order to develop more dynamic therapies that make it easier for patients to maintain long-term treatment. In this context, plants with medicinal properties have been analyzed. The broad-spectrum activity against a range of pathogenic fungi shown by extracts of P. polystachyum, together with the use of antifungal drugs as antiprotozoals, made it important to evaluate the amebicidal activity of these plant extracts against A. castellanii. The greatest activity was observed in the treatment with the hexane fraction, which lysed approximately 66% and 70% of the trophozoites in 48 and 72 h, respectively, preventing encystment.

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Section: Introductionmentioning

confidence: 99%

In vitro evaluation of the amebicidal activity of Pterocaulon polystachyum (Asteraceae) against trophozoites of Acanthamoeba castellanii

et al. 2008

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“…O diagnóstico e tratamento da ceratite por Acanthamoeba representa um desafio aos oftalmologistas (11) . Não há drogas específicas aprovadas para tratar esse processo infeccioso.…”

Section: Discussionunclassified

Citologia de impressão no diagnóstico de infecção corneana por Acanthamoeba: relato de caso

Barros¹,

Mascaro²,

Lowen³

et al. 2007

Arq. Bras. Oftalmol.

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“…Keratitis primarily affects users of contact lenses, which in recent years have greatly increased around the world. The available treatments include aromatic diamidines (hexamidine, pentamidine, or propamidine isothionate), cationic antiseptics, aminoglycosides, imidazoles and polyenes (amphotericin B) (Auran et al 1987;Chomicz et al, 2005;Obeid et al, 2003). Because these drugs do not have great efficacy against the cystic form of this organism, the treatment is long and complex.…”

Section: Introductionmentioning

confidence: 99%

“…Because these drugs do not have great efficacy against the cystic form of this organism, the treatment is long and complex. Thus, the search for new drugs is crucial to develop dynamic therapies and facilitate treatment (Obeid et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

In vitro effect of Acanthospermum australe (Asteraceae) extracts on Acanthamoeba polyphaga trophozoites

Castro

Sauter

Ethur

et al. 2013

Rev. bras. plantas med.

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ABSTRACT:Acanthamoeba is a free-living protozoan widely distributed in the environment, occurring in vegetative trophozoite and resistance cyst stages during its life cycle. It constitutes an etiological agent of Acanthamoeba keratitis, a disease that may cause severe ocular inflammation and blindness. New drugs can be developed from molecules found in plants and thus help in its difficult treatment. Acanthospermum australe (Asteraceae), a plant used in folk medicine, had its effect tested on Acanthamoeba polyphaga. Aqueous and ethanolic extracts of A. austral were obtained from aerial parts for infusion and static maceration, respectively. Concentrations of 10, 5, 2.5, 1.25 and 0.625 mg/ml of the extract were tested against Acanthamoeba polyphaga trophozoites. The cytotoxic effect of the extracts was tested in mammalian cells using the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. RESULTS: The 10 mg/ml concentration of ethanolic extract was lethal to 100% of the A. polyphaga trophozoites in 24 h and both extracts presented cytotoxic effect against mammalian cells. These findings suggest that the A. austral ethanolic extract may have compounds with relevance to the development of new amoebicidal drugs.Keywords: Acanthamoeba polyphaga, amoebicidal activity, Acanthospermum australe. RESUMO:Efeito in vitro de extratos de Acanthospermum australe (Asteraceae) sobre trofozoitos de Acanthamoeba polyphaga. Acanthamoeba é um protozoário de vida livre amplamente distribuído no ambiente, ocorrendo sob a forma trofozoítica (metabolicamente ativa) e cística (de resistência), durante seu ciclo de vida. O protozoário constitui um agente etiológico da Ceratite Amebiana, uma doença que pode causar inflamação ocular severa e cegueira. Novos fármacos podem ser desenvolvidos a partir de moléculas encontradas em plantas e assim ajudar em seu difícil tratamento. Aqui, Acanthospermum australe (Asteraceae), uma planta utilizada na medicina popular, teve seu efeito sobre trofozoítos de Acanthamoeba polyphaga testado. O extrato aquoso e etanólico de A. australe foram obtidos das partes aéreas por infusão e maceração estática, respectivamente. As concentrações 10, 5, 2,5, 1,25 e 0,625 mg/ml dos extratos foram testadas contra trofozoítos do protozoário. O efeito citotóxico dos extratos foi testado em células de mamífero utilizando o ensaio de brometo de 3-[4,5-dimetiltiazol-2-il]-2,5-difeniltetrazólio (MTT). A concentração de 10 mg/ml do extrato etanólico foi letal a 100% dos trofozoítos de A. polyphaga em 24 h e ambos os extratos apresentaram efeito citotóxico contra as células de mamífero. Estes resultados sugerem que o extrato etanólico de A. australe pode ter componentes com relevância para o desenvolvimento de novos fármacos amebicidas.Palavras Chave: Acanthamoeba polyphaga, atividade amebicida, Acanthospermum australe.

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Ceratite bilateral por Acanthamoeba: relato de caso

Cited by 9 publications

References 37 publications

In vitro evaluation of the amebicidal activity of Pterocaulon polystachyum (Asteraceae) against trophozoites of Acanthamoeba castellanii

In vitro evaluation of the amebicidal activity of Pterocaulon polystachyum (Asteraceae) against trophozoites of Acanthamoeba castellanii

Citologia de impressão no diagnóstico de infecção corneana por Acanthamoeba: relato de caso

In vitro effect of Acanthospermum australe (Asteraceae) extracts on Acanthamoeba polyphaga trophozoites

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