Ceramides from Sea Red Rice Bran Improve Health Indicators and Increase Stress Resistance of Caenorhabditis elegans through Insulin/IGF-1 Signaling (IIS) Pathway and JNK-1

Abstract

The antiaging effects of sea red rice bran in vivo, a new saline-tolerant sea rice byproduct containing high levels of ceramides (Cers), remain unknown. This study aimed to explore the antiaging effects exerted by Cers from sea red rice bran on Caenorhabditis elegans, assess its health indicators as well as tolerance, and then reveal the mechanism of action of Cers in prolonging the mean life span through genetic studies. The results indicated that the mean life span of Cers-treated C. elegans were dosedependent in the range of 0.10−0.50 mg/mL. Additionally, Cers improved nematode motility, reduced lipofuscin accumulation, and enhanced resistance to heat stress and antioxidant enzyme activity. Genetic studies showed that Cers treatment had altered nematode gene expression. In addition, insulin/IGF-1 and jnk-1/mitogen-activated protein kinase (MAPK) signaling pathways successfully demonstrated the longevity effects of Cers intake. In short, these results suggest that Cers enhance the resistance of C. elegans and prolong its life span.

Esterification with a Long-Chain Fatty Acid Elevates the Exposure Toxicity of Tigliane Diterpenoids from Euphorbia fischeriana Roots against Nematodes

“…Growth in C. elegans is closely related to lipid accumulation, and lipid accumulation is an indicator of oxidative stress in nematodes. , In this study, both 12-deoxyphorbol-13-hexadecanoate and prostratin reduced lipid accumulation in C. elegans , which was consistent with changes in the transcription expressions of the fat-7 , fat-6 , and fat-2 genes that are related to lipid accumulation. At the same time, lipofuscin accumulation, another indicator associated with oxidative stress in C. elegans , was also affected following treatment with compounds 1 and 2 . These results suggest that the toxic effects of these two isolated tigliane diterpenoids on C. elegans also include oxidative stress.…”

Curcumin supplementation increases longevity and antioxidant capacity in Caenorhabditis elegans

“…ROS and phosphorylation of MAPK are involved in the induction of aging, leading to reduced protein and mRNA levels of acetyl coenzyme A carboxylase 1 (ACC1) ( Marmisolle et al, 2017 ). The over-activation of MAPK enzymes and their lack of responsiveness may develop with aging and may result in promoting irreversible cellular senescence and accelerating the aging process ( Wang et al, 2022 ). It is well known that p38 MAPK has an important role in accelerating cellular senescence.…”