Ceramides and sphingomyelins in skeletal muscles of the rat: content and composition. Effect of prolonged exercise

Dobrzyń, Agnieszka; Górski, Jan

doi:10.1152/ajpendo.00151.2001

Cited by 98 publications

(139 citation statements)

References 29 publications

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“…The muscle biopsies were also analysed for total ceramide fatty acid content and ceramide fatty acid composition via methods previously described [21]. The coefficient of variation for the ceramide content analysis, when analysed in triplicate in rat muscle, was 8.6%.…”

Section: Methodsmentioning

confidence: 99%

Human skeletal muscle ceramide content is not a major factor in muscle insulin sensitivity

et al. 2008

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Aims/hypothesis In skeletal muscle, ceramides may be involved in the pathogenesis of insulin resistance through an attenuation of insulin signalling. This study investigated total skeletal muscle ceramide fatty acid content in participants exhibiting a wide range of insulin sensitivities. Methods The middle-aged male participants (n = 33) were matched for lean body mass and divided into four groups: type 2 diabetes (T2D, n = 8), impaired glucose tolerance (IGT, n = 9), healthy controls (CON, n = 8) and endurancetrained (TR, n = 8). A two step (28 and 80 mU m −2 min −1 ) sequential euglycaemic-hyperinsulinaemic clamp was performed for 120 and 90 min for step 1 and step 2, respectively. Muscle biopsies were obtained from vastus lateralis at baseline, and after steps 1 and 2. Results Glucose infusion rates increased in response to insulin infusion, and significant differences were present between groups (T2D

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Section: Methodsmentioning

confidence: 99%

Human skeletal muscle ceramide content is not a major factor in muscle insulin sensitivity

et al. 2008

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“…Analysis of sphingomyelin signalling pathway in muscle Lipids were extracted according to the method of Folch et al [29] and were then assayed as described in [8,21]. Briefly, ceramides and sphingomyelins were separated by thin-layer chromatography on silica gel plates (Kieselgel 60, 0.22 mm; Merck, Darmstadt, Germany).…”

Section: Measurementsmentioning

confidence: 99%

“…Total ceramide and sphingomyelin level was estimated as the sum of the fatty acid levels of the assessed fraction and was expressed in nmol/g tissue [8,21].…”

Section: Measurementsmentioning

confidence: 99%

“…Muscle activities of membrane Mg 2+ -dependent neutral sphingomyelinase and lysosomal Zn 2+ -independent acid sphingomyelinase were measured according to Liu et al [31], with the use of a radiolabelled substrate, N-cholinemethyl-[ 14 C]sphingomyelin (PerkinElmer Life Sciences, Boston, MA, USA), as in our previous studies [8,21]. The reaction product, N-choline-methyl-[ 14 C]phosphocholine, was mixed with 5 ml of scintillation solution for radioactivity counting.…”

Section: Measurementsmentioning

confidence: 99%

“…1 [6][7][8]. Ceramide, a second messenger in this pathway, is generated during the hydrolysis of plasma membrane sphingomyelin by the enzyme neutral sphingomyelinase or the hydrolysis of lysosomal and endosomal sphingomyelin by acid sphingomyelinase [9].…”

Section: Introductionmentioning

confidence: 99%

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Increased skeletal muscle ceramide level in men at risk of developing type 2 diabetes

et al. 2007

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Aims/hypothesis Intramyocellular lipids, including ceramide, a second messenger in the sphingomyelin signalling pathway, might contribute to the development of insulin resistance. The aim of our study was to assess parameters of the skeletal muscle sphingomyelin signalling pathway in men at risk of developing type 2 diabetes. Methods We studied 12 lean (BMI<25 kg/m 2 ) men without a family history of diabetes (control group), 12 lean male offspring of type 2 diabetic patients, and 21 men with overweight or obesity comprising 12 with NGT (obese-NGT) and nine with IGT (obese-IGT). A euglycaemic-hyperinsulinaemic clamp and a biopsy of vastus lateralis muscle were performed. Ceramide, sphingomyelin, sphinganine and sphingosine levels and sphingomyelinase and ceramidase activities were measured in muscle. Muscle diacylglycerol and triacylglycerol levels were estimated in a subgroup of 27 men (comprising men from all the above groups). Results Compared with the control group, the lean offspring of diabetic patients and the men with overweight or obesity showed lower insulin sensitivity (all p<0.005) and a greater muscle ceramide level (all p<0.01). The obese-IGT group had lower insulin sensitivity (p=0.0018) and higher muscle ceramide (p=0.0022) than the obese-NGT group. There was lower muscle sphingosine level and alkaline ceramidase activity in offspring of diabetic patients (p=0.038 and p=0.031, respectively) and higher sphinganine level in the obese-NGT (p=0.049) and obese-IGT (p=0.002) groups than in the control group. Muscle sphingomyelin was lower (p=0.0028) and neutral sphingomyelinase activity was higher (p=0.00079) in the obese-IGT than in the obese-NGT group. Muscle ceramide was related to insulin sensitivity independently of other muscle lipid fractions. Conclusions/interpretations Ceramide accumulates in muscle of men at risk of developing type 2 diabetes.

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Metabolic Syndrome and Insulin Resistance: Underlying Causes and Modification by Exercise Training

Roberts

Hevener

Barnard

2013

Comprehensive Physiology

451

425

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Metabolic syndrome (MS) is a collection of cardiometabolic risk factors that includes obesity, insulin resistance, hypertension, and dyslipidemia. Although there has been significant debate regarding the criteria and concept of the syndrome, this clustering of risk factors is unequivocally linked to an increased risk of developing type 2 diabetes and cardiovascular disease. Regardless of the true definition, based on current population estimates, nearly 100 million have MS. It is often characterized by insulin resistance, which some have suggested is a major underpinning link between physical inactivity and MS. The purpose of this review is to: (i) provide an overview of the history, causes and clinical aspects of MS, (ii) review the molecular mechanisms of insulin action and the causes of insulin resistance, and (iii) discuss the epidemiological and intervention data on the effects of exercise on MS and insulin sensitivity.

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Ceramides and sphingomyelins in skeletal muscles of the rat: content and composition. Effect of prolonged exercise

Cited by 98 publications

References 29 publications

Human skeletal muscle ceramide content is not a major factor in muscle insulin sensitivity

Human skeletal muscle ceramide content is not a major factor in muscle insulin sensitivity

Increased skeletal muscle ceramide level in men at risk of developing type 2 diabetes

Metabolic Syndrome and Insulin Resistance: Underlying Causes and Modification by Exercise Training

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