Ceramide triggers intracellular calcium release  via the IP<sub>3</sub> receptor in<i>Xenopus laevis</i>oocytes

Kobrinsky, Evgeny; Spielman, Andrew I.; Rosenzweig, Sanford; Marks, Andrew R.

doi:10.1152/ajpcell.1999.277.4.c665

Cited by 86 publications

(21 citation statements)

References 54 publications

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“…Furthermore, ceramide addition to Xenopus oocytes released [Ca 21 ] in through Gq, PLCb, and IP3 receptor activation (83). Because this G protein pathway is not thought to be involved in [Ca 21 ] in release as initiated by sperm (84), ceramide may not play a role in the calcium release at fertilization.…”

Section: Sm and Ceramidementioning

confidence: 99%

Lipid levels in sperm, eggs, and during fertilization in Xenopus laevis

Petcoff

Holland

Stith

2008

Journal of Lipid Research

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Critical developmental periods, such as fertilization, involve metabolic activation, membrane fusion events such as sperm-egg or plasma membrane-cortical granule merger, and production and hydrolysis of phospholipids. However, there has been no large-scale quantification of phospholipid changes during fertilization. Using an enzymatic assay, traditional FA analysis by TLC and gas chromatography, along with a new method of phospholipid measurement involving HPLC separation and evaporative light-scattering detection, we report lipid levels in eggs, sperm, and during fertilization in Xenopus laevis. Sperm were found to contain different amounts of phospholipids as compared with eggs. During fertilization, total phosphatidylinositol, lysophosphatidylcholine, sphingomyelin, and phosphatidylserine decreased, and ceramide increased, whereas there was no change in phosphatidylcholine, cardiolipin, or phosphatidylethanolamine. FA analysis of phospholipids found numerous changes during fertilization. Because there is an increase in sn-1,2-diacylglycerol at fertilization, the FAs associated with this increase and the source of the increase in this neutral lipid were examined. Finally, activation of phospholipase C, phospholipase D, phospholipase A2, autotoxin, and sphingomyelinase at fertilization is discussed. Major goals of the LIPID Metabolites and Pathways Strategy (LIPID MAPS) are to "separate and detect all of the lipids in a specific cell," "to quantitate each of the lipid metabolites present and determine the changes in their levels," and to study lipid metabolic pathways during biological events (1). Characterizing changes in the lipid composition during defined biological events is part of the concept of "functional lipidomics" (2). These fundamental, descriptive studies will facilitate our understanding of how lipids regulate membrane fluidity and fusion, lipid rafts, the cytoskeleton, kinases, and protein binding to membranes (e.g., 3-5) to induce pleiotropic cellular effects.About 15 years ago, quantification of lipid mass changes during maturation of the oocyte to the fertilizable egg, and during fertilization of the egg by sperm, was begun in Xenopus laevis (6-9). Critical developmental events can be isolated in vitro with this model system, and large numbers of gametes can be obtained to provide sufficient amounts of second messengers and lipids. During fertilization, membrane bending and fusion are associated with the acrosome reaction of sperm (wherein the large sperm acrosome undergoes exocytosis upon interaction with layers that cover the egg), with sperm-egg merger, and with subsequent fertilization events such as cortical granule exocytosis (10, 11). Furthermore, both Xenopus and human eggs are arrested in meiosis II (12).Compared with oocytes, one disadvantage of studying eggs is that they have a short life, much less than the estimated time for lipid precursor incorporation to near equilibrium levels (6, 7). Thus, lipid analysis based on label incorporation or turnover is not reliable, and ...

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Section: Sm and Ceramidementioning

confidence: 99%

Lipid levels in sperm, eggs, and during fertilization in Xenopus laevis

Petcoff

Holland

Stith

2008

Journal of Lipid Research

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“…In our studies we used human recombinant TNF␣, which in rodent cells activates the p55 TNF␣ receptor and signaling pathways that generate ceramide (42,43); ceramide has been shown to trigger intracellular calcium release through IP 3 receptors (44). It appears that one factor controlling the cell susceptibility to ceramide-induced apoptosis is the [Ca 2ϩ ] er (39).…”

Section: Inhibition Of Ip 3 -Induced Calcium Release Protects Relamentioning

confidence: 99%

Suppression of Calcium Release from Inositol 1,4,5-Trisphosphate-sensitive Stores Mediates the Anti-apoptotic Function of Nuclear Factor-κB

Camandola

Cutler

Gary

et al. 2005

Journal of Biological Chemistry

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“…To account for this function, observations in model and cell membranes reported the flipflop movement of ceramide (42,43), gaining access to the inner leaflet of the plasma membrane to interact with specific targets. In agreement with this concept, extracellular NSMase from S. aureus has been shown to increase cytoplasmic Ca 21 via the inositol-3-phosphate receptor Ca 21 release channel on the endoplasmic reticulum, leading to oocyte maturation (51). Moreover, another study reported that the inhibition of glycosphingolipid synthesis by 1-phenyl-2-decanoylamino-3-morpholino-1-propanol (PDMP), an inhibitor of the glucosylceramide synthase (GCS) (52), activated p34 cdc2 in Xenopus oocytes and induced GVBD and maturation (45).…”

Section: Discussionmentioning

confidence: 62%

Neutral sphingomyelinase-induced ceramide triggers germinal vesicle breakdown and oxidant-dependent apoptosis in Xenopus laevis oocytes

Coll

Morales

Fernández-Checa

et al. 2007

Journal of Lipid Research

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Ceramide regulates many cellular processes, including cell growth, differentiation, and apoptosis. Although the effects of exogenous bacterial neutral sphingomyelinase (SMase) in Xenopus laevis oocytes have been investigated, its microinjection into oocytes has not been reported previously. Thus, we compared the incubation versus microinjection of the neutral Bacillus cereus sphingomyelinase (bSMase) to examine whether the topology of ceramide generation determines its effects on the fate of oocytes. In agreement with previous findings, incubation of mature stage VI oocytes with bSMase increased ceramide levels in oocyte extracts over time, causing the germinal vesicle breakdown indicative of maturation, without evidence of cytotoxicity. In contrast, bSMase microinjection, which increased ceramide levels in a time-and dose-dependent manner, resulted in oocyte apoptosis characterized by reactive oxygen species (ROS) generation, reduced glutathione (GSH) depletion in cytosol and mitochondria, release of cytochrome c and Smac/Diablo from mitochondria, and caspase-3 activation. Microinjection of acidic SMase from human placenta recapitulated the apoptotic effects of bSMase microinjection. Preincubation of oocytes with GSH-ethyl ester before bSMase microinjection prevented ROS generation and mitochondrial downstream events, thus protecting oocytes from bSMaseinduced death. These findings show a divergent action of bSMase-induced ceramide on oocyte maturation or apoptosis depending on the intracellular site where ceramide is generated.-Coll, O., A. Morales, J. C. Fernández-Checa, and C. Garcia-Ruiz. Neutral sphingomyelinase-induced ceramide triggers germinal vesicle breakdown and oxidantdependent apoptosis in Xenopus laevis oocytes. J. Lipid Res.

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Ceramide triggers intracellular calcium release via the IP₃ receptor inXenopus laevisoocytes

Cited by 86 publications

References 54 publications

Lipid levels in sperm, eggs, and during fertilization in Xenopus laevis

Lipid levels in sperm, eggs, and during fertilization in Xenopus laevis

Suppression of Calcium Release from Inositol 1,4,5-Trisphosphate-sensitive Stores Mediates the Anti-apoptotic Function of Nuclear Factor-κB

Neutral sphingomyelinase-induced ceramide triggers germinal vesicle breakdown and oxidant-dependent apoptosis in Xenopus laevis oocytes

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Ceramide triggers intracellular calcium release via the IP3 receptor inXenopus laevisoocytes

Cited by 86 publications

References 54 publications

Lipid levels in sperm, eggs, and during fertilization in Xenopus laevis

Lipid levels in sperm, eggs, and during fertilization in Xenopus laevis

Suppression of Calcium Release from Inositol 1,4,5-Trisphosphate-sensitive Stores Mediates the Anti-apoptotic Function of Nuclear Factor-κB

Neutral sphingomyelinase-induced ceramide triggers germinal vesicle breakdown and oxidant-dependent apoptosis in Xenopus laevis oocytes

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Ceramide triggers intracellular calcium release via the IP₃ receptor inXenopus laevisoocytes