Ceramide cross‐linking leads to pore formation: Potential mechanism behind CAP enhancement of transdermal drug delivery

Paal, Jonas Van der; Fridman, Gregory; Bogaerts, Annemie

doi:10.1002/ppap.201900122

Cited by 5 publications

(8 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It also facilitates the transport of nutrients into the cell from the outside and removes toxic substances from the intracellular environment [ 44 , 45 ]. Structural changes induced by RONS in the membrane can disrupt its normal permeability to certain substances [ 46 , 47 ], including RONS produced by CAP [ 48 , 49 , 50 ]. Increased entry of RONS into the cell disrupts the balance between intracellular RONS levels and the antioxidant system, resulting in oxidative stress [ 51 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of Nitro-Oxidative Stress on Biomolecules: Part 1—Non-Reactive Molecular Dynamics Simulations

Ghasemitarei,

Ghorbi,

Yusupov

et al. 2023

Biomolecules

Self Cite

View full text Add to dashboard Cite

Plasma medicine, or the biomedical application of cold atmospheric plasma (CAP), is an expanding field within plasma research. CAP has demonstrated remarkable versatility in diverse biological applications, including cancer treatment, wound healing, microorganism inactivation, and skin disease therapy. However, the precise mechanisms underlying the effects of CAP remain incompletely understood. The therapeutic effects of CAP are largely attributed to the generation of reactive oxygen and nitrogen species (RONS), which play a crucial role in the biological responses induced by CAP. Specifically, RONS produced during CAP treatment have the ability to chemically modify cell membranes and membrane proteins, causing nitro-oxidative stress, thereby leading to changes in membrane permeability and disruption of cellular processes. To gain atomic-level insights into these interactions, non-reactive molecular dynamics (MD) simulations have emerged as a valuable tool. These simulations facilitate the examination of larger-scale system dynamics, including protein-protein and protein-membrane interactions. In this comprehensive review, we focus on the applications of non-reactive MD simulations in studying the effects of CAP on cellular components and interactions at the atomic level, providing a detailed overview of the potential of CAP in medicine. We also review the results of other MD studies that are not related to plasma medicine but explore the effects of nitro-oxidative stress on cellular components and are therefore important for a broader understanding of the underlying processes.

show abstract

Section: Introductionmentioning

confidence: 99%

Effects of Nitro-Oxidative Stress on Biomolecules: Part 1—Non-Reactive Molecular Dynamics Simulations

Ghasemitarei,

Ghorbi,

Yusupov

et al. 2023

Biomolecules

Self Cite

View full text Add to dashboard Cite

show abstract

“…One may suspect that besides the reactive species delivery and action on bacteria, the bacteria on the central zone of the plasma jet impact zone will rapidly be inactivated as there the electric field strength is large enough, while those on the more outer locations will require the accumulation of a larger number of electric field pulses of lower strength. Such combinatory and threshold-dependent action of pulsed electric field with reactive species was recently reported [48,[58][59][60][61] during plasma treatment of various cell lines.…”

Section: Inactivation Of Staphylococcus Aureusmentioning

confidence: 57%

Anti-Bacterial Action of Plasma Multi-Jets in the Context of Chronic Wound Healing

et al. 2021

View full text Add to dashboard Cite

This work is a contribution to the development and implementation of non-thermal plasma technology for decontamination in the perspective of nosocomial and chronic wound innovative therapies. Multi jets devices based on Plasma Gun® technology in static and scanning operation modes and bacterial lawns inoculated with resistant and non-resistant bacterial strains were designed and used. A pilot toxicity study exploring plasma treatment of wound bearing patients, performed with a low voltage plasma applicator, is documented as a first step for the translation of in vitro experiments to clinical care. Bacterial inactivation was demonstrated for Staphylococcus aureus, Pseudomonas aeruginosa and drug resistant S. aureus, P. aeruginosa and Escherichia Coli strains collected from patient wounds at Orleans (France) hospital. A few square centimeter large contaminated samples were inactivated following a single plasma exposure as short as one minute. Samples inoculated with a single but also a mix of three resistant pathogens were successfully inactivated not only right after their contamination but for mature lawns as well. Similar bactericidal action was demonstrated for antibiotic-resistant and non-resistant P. aeruginosa. The time exposure dependent increase of the inhibition spots, following multi jets exposure, is discussed as either the accumulation of reactive species or the likely combinatory action of both the reactive species and transient electric field delivery on inoculated samples.

show abstract

“…45 CAP enhances the transcellular route by increasing the permeability of the plasma membrane through the peroxidation of lipid components in the phospholipid bilayer. [46][47][48][49][50][51] CAP also enhances intercellular delivery by suppressing the expression of cell adhesion molecules, such as E-cadherin. 37,52 Our quantification analysis confirmed that the LICAP device used in the present study could precisely adjust the reactive species production in the target solution (Figure 8A-D).…”

Section: Discussionmentioning

confidence: 99%

“…The additive effect of concurrent therapy is likely related to the ability of CAP to enhance transdermal drug delivery via transcellular and intercellular routes 45 . CAP enhances the transcellular route by increasing the permeability of the plasma membrane through the peroxidation of lipid components in the phospholipid bilayer 46–51 . CAP also enhances intercellular delivery by suppressing the expression of cell adhesion molecules, such as E‐cadherin 37,52 …”

Section: Discussionmentioning

confidence: 99%

Low‐intensity cold atmospheric plasma reduces wrinkles on photoaged skin through hormetic induction of extracellular matrix protein expression in dermal fibroblasts

et al. 2022

View full text Add to dashboard Cite

Background: Recent evidence indicates that cold atmospheric plasma (CAP) can upregulate the production of extracellular matrix (ECM) proteins in dermal fibroblasts and enhance transdermal drug delivery when applied at a low intensity.Objectives: The aim of this study was to evaluate the effect of low-intensity CAP (LICAP) on photoaging-induced wrinkles in an animal model and the expression profiles of ECM proteins in human dermal fibroblasts. Methods: Each group was subjected to photoaging induction and allocated to therapy (LICAP, topical polylactic acid (PLA), or both). The wrinkles were evaluated via visual inspection, quantitative analysis, and histology. The expression of collagen I/III and fibronectin was assessed using reverse transcription-quantitative polymerase chain reaction, western blot analysis, and immunofluorescence. The amount of aqueous reactive species produced by LICAP using helium and argon gas was also measured. Results: Wrinkles significantly decreased in all treatment groups compared to those in the untreated control. The differences remained significant for at least 4 weeks. Dermal collagen density increased following LICAP and PLA application. LICAP demonstrated a hormetic effect on ECM protein expression in human dermal fibroblasts. The production of reactive species increased, showing a biphasic pattern, with an initial linear phase and a slow saturation phase. The initial linearity was sustained for a longer time in the helium plasma (~60 s) than in the argon plasma (~15 s). Conclusion: LICAP appears to be a novel treatment option for wrinkles on the photodamaged skin. This treatment effect seems to be related to its hormetic effect on dermal ECM production.

show abstract

Ceramide cross‐linking leads to pore formation: Potential mechanism behind CAP enhancement of transdermal drug delivery

Cited by 5 publications

References 34 publications

Effects of Nitro-Oxidative Stress on Biomolecules: Part 1—Non-Reactive Molecular Dynamics Simulations

Effects of Nitro-Oxidative Stress on Biomolecules: Part 1—Non-Reactive Molecular Dynamics Simulations

Anti-Bacterial Action of Plasma Multi-Jets in the Context of Chronic Wound Healing

Low‐intensity cold atmospheric plasma reduces wrinkles on photoaged skin through hormetic induction of extracellular matrix protein expression in dermal fibroblasts

Contact Info

Product

Resources

About