Ceramide and sphingosine have an antagonistic effect on the plasma-membrane Ca2+-ATPase from human erythrocytes

Colina, Claudia; Cervino, Vincenza; Benaím, Gustavo

doi:10.1042/0264-6021:3620247

Cited by 17 publications

(12 citation statements)

References 38 publications

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“…However, our experiments using two different Shaker-channel mutants, one with altered voltage dependence and one nonconducting, clearly show that neither cellular hyperpolarization nor the Shaker channel K ϩ conductance is the critical event. The activity of several membrane proteins like channels and pumps are affected by the composition of the lipid bilayer (21,22), the interaction with other membrane proteins (23,24), and by membrane protein dilution (possible via interactions with the cytoskeleton) (25). It is possible that the Shaker channel protein affects K ϩ efflux through direct or indirect interactions with apoptosis-associated channels or pumps.…”

Section: Discussionmentioning

confidence: 99%

Intracellular K+ Concentration Decrease Is Not Obligatory for Apoptosis

Börjesson

Englund

Asif

et al. 2011

Journal of Biological Chemistry

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Section: Discussionmentioning

confidence: 99%

Intracellular K+ Concentration Decrease Is Not Obligatory for Apoptosis

Börjesson

Englund

Asif

et al. 2011

Journal of Biological Chemistry

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“…It has been shown that Cer with short fatty acid chains (C 2 -and C 8 -Cer) activates both soluble and native erythrocyte Ca 2ϩ -ATPase in a concentration range near and above the critical micellar concentration (42). In this work we explored the possibility that, in addition to a direct effect at high concentrations, probably related to physical changes in the pump molecule, Cer, in the nanomolar range expected for a second messenger, could be capable of interacting with plasma membrane signaling cascades in the close vicinity that are functionally coupled to Ca 2ϩ -ATPase.…”

Section: Discussionmentioning

confidence: 99%

Ceramide Is a Potent Activator of Plasma Membrane Ca2+-ATPase from Kidney Proximal Tubule Cells with Protein Kinase A as an Intermediate

Cabral

Wengert

Ressurreição

et al. 2007

Journal of Biological Chemistry

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The kidney proximal tubules are involved in reabsorbing twothirds of the glomerular ultrafiltrate, a key Ca 2؉ -modulated process that is essential for maintaining homeostasis in body fluid compartments. The basolateral membranes of these cells have a Ca 2؉ -ATPase, which is thought to be responsible for the fine regulation of intracellular Ca 2؉ levels. In this paper we show that nanomolar concentrations of ceramide (Cer 50 ‫؍‬ 3.5 nM), a natural product derived from sphingomyelinase activity in biological membranes, promotes a 50% increase of Ca Important molecular transport processes take place across the epithelium of kidney proximal tubules. The basolateral membranes (BLM) 4 of kidney proximal tubules cells contain different active transporters, or ion pumps, such as the very abundant Na ϩ K ϩ -ATPase, which is considered to be the molecular machinery responsible for Na ϩ reabsorption (1). Other ion pumps are not so numerous, but some, such as the plasma membrane calcium pump, play important roles in the fine regulation of intracellular ion concentrations. Our group has recently shown that Ca 2ϩ -ATPase is exclusively located and active in caveolin-cholesterol-rich membrane microdomains or lipid rafts in the kidney BLM (2). These membranes have also been shown to house different cell signaling systems that are initiated by the activation of either different lipid kinases, with further generation of bioactive molecules (3, 4), or protein kinases associated with the BLM (5-8).The location of the BLM Ca 2ϩ -ATPase in caveolin-cholesterol-rich domains adds ceramides to the emerging potential regulatory network in these membranes, because those microdomains are also rich in sphingolipids (9). Rafts are thought to be present in the outer leaflet of the cell membrane, where sphingomyelin, the precursor of ceramide (Cer) in a pathway catalyzed by sphingomyelinases, appears to be predominantly located. An important concept is that the assembly of the outer leaflet lipid rafts would alter the inner leaflet, thus enabling the different steps required for signal transduction to be coordinated (for review, see Ref. 10).Many studies during the past decade have shown that smaller lipid rafts are merged into large membrane domains when sphingomyelin is hydrolyzed and Cer is generated (Refs. 11 and 12; see also Ref. 10 for review). The generation of Cer molecules and their self-association leads to dramatic changes in plasma membranes with further formation of small Cer-rich microdomains, which are able to fuse spontaneously to others resulting in large domains called platforms (12)(13)(14). Therefore, Cer-enriched microdomains seem to play an important role in facilitating and amplifying signaling processes, via different types of cell surface receptors, resulting in clusters of receptors and other cell signaling machinery that facilitate the effective transduction of different signals (10).The importance of Cer in different cell processes is not only related to its physicochemical properties. It can be also considered a c...

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“…The skeletal muscle SR Ca 2+ -ATPase is stimulated by jasmine and jasmonate and increase the rate of dephosphorylation of the ATPase [143]. Reports show that ceramide stimulates the plasma membrane Ca 2+ -ATPase activity in a dose dependent manner and an additive effect in activation was observed in presence of calmodulin and ethanol [144]. Ceramide affects the affinity for Ca 2+ and V max of the enzyme, and also stimulates Ca 2+ transport in inside-out plasma membrane vesicles from erythrocytes.…”

Section: Regulation Ofmentioning

confidence: 99%

Endogenous modulators in the regulation of ion transporting enzymes: structure, function, interactions, recent advancements and future perspectives

Sen¹

2011

ABC

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A prerequisite for life is the ability to uphold electrochemical imbalance across biomembranes. Ion transporting enzymes, known as specific pumps, are responsible for the transport of various ions across cell membranes to sustain the same. In all eukaryotes, the plasma membrane potential and secondary transport systems are maintained by the activity of P-type ion transporting enzymes, commonly known as ATPase membrane pumps. Malfunction of pumps leads to various cell disorders and subsequently diseases like cardiac problems, renal malfunctionings, diabetes, cataract, even cancer. Activities/functions of these pumps are regulated either by exogenous agents or by endogenous substances like proteins, peptides, hormones, etc., which are collectively known as modulators. Some of these endogenous modulators may be useful for developing drugs depending on the nature of regulation. For more than last two decades, researchers across the globe are exploring the mechanism of action of different endogenous modulators on these ion transporting enzymes with the aim of developing target-specific drugs. In this review, we have discussed recent advances in our understanding of ATPase pumps, e.g., Ca 2+ -, Na

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Ceramide and sphingosine have an antagonistic effect on the plasma-membrane Ca2+-ATPase from human erythrocytes

Cited by 17 publications

References 38 publications

Intracellular K+ Concentration Decrease Is Not Obligatory for Apoptosis

Intracellular K+ Concentration Decrease Is Not Obligatory for Apoptosis

Ceramide Is a Potent Activator of Plasma Membrane Ca2+-ATPase from Kidney Proximal Tubule Cells with Protein Kinase A as an Intermediate

Endogenous modulators in the regulation of ion transporting enzymes: structure, function, interactions, recent advancements and future perspectives

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