Cephamycin C biosynthesis in Streptomyces cattleya: nitrogen source regulation

Abstract

The production of cephamycin C by Streptomyces cattleya varies with the use of asparagine, glutamine or ammonium as nitrogen sources. Hydroxylase and expandase activities were demonstrated for the first time with this species. A study of the biosynthetic regulation of these enzymes by two different nitrogen sources, glutamine and asparagine, was carried out. Asparagine proved to be a better nitrogen source, both for enzymatic biosynthesis and production of cephamycin C. Moreover, an excess of asparagine in the culture environment provokes, simultaneously, a reduction in cephamycin C production and a decrease in the biosynthesis of expandase and hydroxylase.

Optimization of culture conditions of Streptomyces rochei (MTCC 10109) for the production of antimicrobial metabolites

“…Similar observations have been reported by many investigators (Khaoua et al 1991;Mansour et al 1996;Chattopadhyay & Sen 1997;Vandana et al 2008). In the present study, phosphate (KH 2 PO 4 ) was not favorable for metabolite production; an excessive amount of inorganic phosphate suppresses the production of antibiotics (Zinzina & Efimova 1979;Kishimoto et al 1996).…”

Influence on dithiolopyrrolone antibiotic production by organic acids in Saccharothrix algeriensis NRRL B-24137

“…These organic acids are used directly as precursors in the biosynthesis of TIP and BUP, and it is reasonable that they are formed selectively in the presence of these organic acids. These results are similar to those found by many authors who reported that the production of monensins A and B by S. cinnamonensis, APHE-1 and APHE-2 by S. griseocarneus, erythromycin by S. erythreus and spiramycin by S. ambofaciens is induced by propionate and butyrate as precursors [12,15,17,18].…”

“…In bacteria, two enzyme systems can convert short-chain fatty acids to acylCoA: the acyl-CoA synthetase system and the acyl-kinase system coupled with acylphosphotransferase. The synthesis of these two enzymatic systems was induced by short-chain fatty acids [12]. These acids are then decarboxylated and converted to their coenzyme A thioesters.…”

Homologous expression of aspartokinase (ask) gene inStreptomyces clavuligerusand itshom-deleted mutant: Effects on cephamycin C production

“…In our previous study, the growth of the AK39 strain was rather poor in chemically-defined medium, CDM, in spite of a two-fold increased specific level of cephamycin C. 17 Some inhibitory effects of glycerol on cephamycin C production 18,19 as well as improvement of growth and cephamycin C yields by L-asparagine 20,21 have been previously reported. In the present study, the composition of CDM was submitted to several modifications to improve the growth of this strain and their transformants.…”