CEP55 promotes cell proliferation and inhibits apoptosis via the PI3K/Akt/p21 signaling pathway in human glioma U251 cells

Li, Feng; Jin, Dayong; Tang, Chuanxi; Gao, Dianshuai

doi:10.3892/ol.2018.7934

Cited by 20 publications

(20 citation statements)

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“…The CEP128 gene is related to the PI3K-Akt-mTOR signaling pathway. Centrosomal protein 128 ( CEP128 ) is part of the centrosomal protein family, including CEP55 that has been implicated in cancer progression [54]. Mutations within CEP128 have been associated with an aggressive type of lymphoma, the diffuse large B-cell lymphoma (DLBCL) [55].…”

Section: Discussionmentioning

confidence: 99%

Genetic control of longissimus dorsi muscle gene expression variation and joint analysis with phenotypic quantitative trait loci in pigs

et al. 2019

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BackgroundEconomically important growth and meat quality traits in pigs are controlled by cascading molecular events occurring during development and continuing throughout the conversion of muscle to meat. However, little is known about the genes and molecular mechanisms involved in this process. Evaluating transcriptomic profiles of skeletal muscle during the initial steps leading to the conversion of muscle to meat can identify key regulators of polygenic phenotypes. In addition, mapping transcript abundance through genome-wide association analysis using high-density marker genotypes allows identification of genomic regions that control gene expression, referred to as expression quantitative trait loci (eQTL). In this study, we perform eQTL analyses to identify potential candidate genes and molecular markers regulating growth and meat quality traits in pigs.ResultsMessenger RNA transcripts obtained with RNA-seq of longissimus dorsi muscle from 168 F2 animals from a Duroc x Pietrain pig resource population were used to estimate gene expression variation subject to genetic control by mapping eQTL. A total of 339 eQTL were mapped (FDR ≤ 0.01) with 191 exhibiting local-acting regulation. Joint analysis of eQTL with phenotypic QTL (pQTL) segregating in our population revealed 16 genes significantly associated with 21 pQTL for meat quality, carcass composition and growth traits. Ten of these pQTL were for meat quality phenotypes that co-localized with one eQTL on SSC2 (8.8-Mb region) and 11 eQTL on SSC15 (121-Mb region). Biological processes identified for co-localized eQTL genes include calcium signaling (FERM, MRLN, PKP2 and CHRNA9), energy metabolism (SUCLG2 and PFKFB3) and redox hemostasis (NQO1 and CEP128), and results support an important role for activation of the PI3K-Akt-mTOR signaling pathway during the initial conversion of muscle to meat.ConclusionCo-localization of eQTL with pQTL identified molecular markers significantly associated with both economically important phenotypes and gene transcript abundance. This study reveals candidate genes contributing to variation in pig production traits, and provides new knowledge regarding the genetic architecture of meat quality phenotypes.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-5386-2) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Genetic control of longissimus dorsi muscle gene expression variation and joint analysis with phenotypic quantitative trait loci in pigs

et al. 2019

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“…For example, the down-regulation of CEP55 can inhibit the proliferation of breast cancer cells 23. CEP55 can promote proliferation and inhibit apoptosis of glioma cells through PI3K/Akt/p21 signal pathways 6. In addition, the expression of CEP55 is inversely correlated with poor prognosis in patients with advanced esophageal squamous cell carcinoma and NSCLC 24,25.…”

Section: Discussionmentioning

confidence: 99%

“…Centrosome-related protein CEP55 (centrosomal protein, 55 kD) is one of the coiled coil protein family members, and its main functions are to anchor the microtubule and polymerize related proteins, participate in the spindle formation, and then regulate cell proliferation 5. It has been found that CEP55 is expressed in both normal tissues and tumor cells and coupled with centrosomes and intermediates in the cell cycle which plays a role in regulating cell cycle after phosphorylation 6,7. In addition, CEP55 overexpression has been shown to be significantly correlated with tumor staging, invasiveness and metastasis of many malignant tumors,7,8 and can be used as a biomarker for tumor occurrence, progression and prognosis.…”

Section: Introductionmentioning

confidence: 99%

<p>MiR-195-5p Inhibits Proliferation and Induces Apoptosis of Non-Small Cell Lung Cancer Cells by Targeting CEP55</p>

Luo¹,

Pan²,

Jin³

et al. 2019

OTT

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Objective: This study aims to explore whether miR-195-5p can inhibit proliferation and induce apoptosis of non-small cell lung cancer (NSCLC) cells by targeting CEP55. Methods: qRT-PCR was used to measure the expression of miR-195-5p in NSCLC cells. MTT assay, colony formation assay, and flow cytometry were used to detect the role of miR-195-5p in NSCLC cells. Western blot was used to measure the protein expression of CEP55, Bax and Bcl-2 in cells. Dual-Luciferase assay was performed to verify the relationship between miR-195-5p and CEP55. Results: The expression of miR-195-5p was higher in human normal lung cell lines than in NSCLC cells. MiR-195-5p overexpression inhibited cell proliferation, which could block the cell cycle of A549 cell line in the G 0 /G 1 phase. Moreover, overexpression of miR-195-5p increased cell apoptotic rate of A549 cell lines, with the expression of pro-apoptotic protein Bax up-regulated and that of the anti-apoptotic protein Bcl-2 down-regulated. The Dual-Luciferase assay showed that miR-195-5p could specifically target CEP55. Furthermore, CEP55 was down-regulated in NSCLC cells. Overexpression of CEP55 enhanced the proliferation and colony formation ability of A549 cell line. Overexpression of CEP55 can reverse the inhibitory effect of miR-195-5p. Conclusion: MiR-195-5p inhibits proliferation and induces apoptosis of NSCLC cells by negatively regulating CEP55.

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“…High CEP55 expression results in disordered cytokinesis and an increased number of unstable binucleated cells; these are oncogenic characteristics in tumorigenesis (34,35). Numerous studies have demonstrated that overexpression of CEP55 is associated with the pathological processes of several human malignances, including hepatocellular carcinoma (36–38), colon carcinoma (39), breast cancer (40–42), lung cancer (43,44), glioma (45–47), nasopharyngeal carcinoma (48), oral cavity squamous cell carcinoma (49), osteosarcoma (50), head and neck cancer (51), anaplastic thyroid carcinoma (52), cervical cancer (53), esophageal squamous cell carcinoma (54,55), pancreatic cancer (56) and prostate cancer (57,58). In addition, high CEP55 expression in colorectal cancer downregulates the tumor susceptibility 101 gene in a post-transcriptional manner, and knockdown of CEP55 inhibits cell growth and increases apoptosis (39).…”

Section: Discussionmentioning

confidence: 99%

Diagnostic and prognostic value of CEP55 in clear cell renal cell carcinoma as determined by bioinformatics analysis

Zhou

Liu

et al. 2019

Mol Med Report

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Clear cell renal cell carcinoma (ccRCC) is one of the most common types of malignant adult kidney tumor. Tumor recurrence and metastasis is the primary cause of cancer-associated mortality in patients with ccRCC. Therefore, identification of efficient diagnostic and prognostic molecular markers may improve survival times. The GSE46699, GSE36895, GSE53000 and GSE53757 gene datasets were downloaded from the Gene Expression Omnibus database and contained 196 ccRCC samples and 164 adjacent normal kidney samples. Bioinformatics analysis was used to integrate the four microarray datasets to identify and analyze differentially expressed genes. Functional analysis revealed that there were 12 genes associated with cancer, based on the tumor-associated gene database. Erb-B2 receptor tyrosine kinase 4, centrosomal protein 55 (CEP55) and vascular endothelial growth factor A are oncogenes, all of which were associated with tumor stage, whereas only CEP55 was significantly associated with survival time as determined by Gene Expression Profiling Interactive Analysis. The mRNA expression levels of CEP55 in ccRCC samples were significantly higher than those observed in adjacent normal kidney tissues based on The Cancer Genome Atlas data and reverse transcription-polymerase chain reaction results. The receiver operating characteristic curve analysis revealed that CEP55 may be considered a diagnostic biomarker for ccRCC with an area under the curve of >0.85 in the training and validation sets. High CEP55 expression was strongly associated with sex, histological grade, stage, T classification, N classification and M classification. Univariate and multivariate Cox proportional hazards analyses demonstrated that CEP55 expression was an independent risk factor for poor prognosis. In addition, gene set enrichment analysis indicated that high CEP55 expression was associated with immunization, cell adhesion, inflammation, the Janus kinase/signal transducer and activator of transcription signaling pathway and cell proliferation. In conclusion, CEP55 was increased in ccRCC samples, and may be considered a potential diagnostic and prognostic biomarker for ccRCC.

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CEP55 promotes cell proliferation and inhibits apoptosis via the PI3K/Akt/p21 signaling pathway in human glioma U251 cells

Cited by 20 publications

References 38 publications

Genetic control of longissimus dorsi muscle gene expression variation and joint analysis with phenotypic quantitative trait loci in pigs

Genetic control of longissimus dorsi muscle gene expression variation and joint analysis with phenotypic quantitative trait loci in pigs

<p>MiR-195-5p Inhibits Proliferation and Induces Apoptosis of Non-Small Cell Lung Cancer Cells by Targeting CEP55</p>

Diagnostic and prognostic value of CEP55 in clear cell renal cell carcinoma as determined by bioinformatics analysis

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