CEP5 and XIP1/CEPR1 regulate lateral root initiation in Arabidopsis

Roberts, Ianto; Smith, Stephanie; Stes, Elisabeth; Rybel, Bert De; Staes, An; Cotte, Brigitte van de; Njo, Maria; Dedeyne, Lise; Demol, Hans; Lavenus, Julien; Audenaert, Dominique; Gevaert, Kris; Beeckman, Tom; Smet, Ive De

doi:10.1093/jxb/erw231

Cited by 84 publications

(104 citation statements)

References 50 publications

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“…3a). As expected, genes previously characterized as CC-related, including SULFATE TRANSPORTER 2;1 (SULTR2;1) 9 , SODIUM POTASSIUM ROOT DEFECTIVE 1 (NaKR1) 10 , C-TERMINALLY ENCODED 11,12 and MYB-RELATED PROTEIN 1 (MYR1) 13 , showed similar expression patterns to SUC2 and SAPL (Fig. 2d).…”

Section: Visual-cc Can Induce Known Cc-related Genes Expressionsupporting

confidence: 82%

GSK3 activity is a cell fate switch that balances the ratio of vascular cell type

Tamaki

Oya

Naito

et al. 2020

Preprint

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The phloem transports photosynthetic assimilates and signalling molecules. It mainly consists of sieve elements (SEs), which act as "highways" for transport, and companion cells (CCs), which serve as "gates" to load/unload cargos. Though SEs and CCs function together, it remains unknown what determines the ratio of SE /CC in the phloem. In this study, we develop a novel culture system for CC differentiation named VISUAL-CC, which reconstitutes the SE-CC complex formation. Comparative expression analysis in VISUAL-CC reveals that SE and CC differentiation tends to show negative correlation, while total phloem differentiation is unchanged. This varying SE/CC ratio is largely dependent on GSK3 kinase activity. Indeed, gsk3 hextuple mutants possess much more SEs and less CCs in planta. Conversely, gsk3 gain-of-function mutants induced by phloem-specific promoter partially increased the CC ratio. Taken together, GSK3 activity appears to function as a cell fate switch in the phloem, thereby balancing the SE/CC ratio.

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Section: Visual-cc Can Induce Known Cc-related Genes Expressionsupporting

confidence: 82%

GSK3 activity is a cell fate switch that balances the ratio of vascular cell type

Tamaki

Oya

Naito

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…3a). As expected, genes previously characterized as CC-related, including SULFATE TRANSPORTER 2;1 (SULTR2;1) 9 , SODIUM POTASSIUM ROOT DEFECTIVE 1 (NaKR1) 10 , C-TERMINALLY ENCODED PEP-TIDE RECEPTOR (CEPR1)/XYLEM INTERMIXED WITH PHLOEM 1 (XIP1) 11,12 , and MYB-RELATED PROTEIN 1 (MYR1) 13 , showed similar expression patterns to SUC2 and SAPL ( Fig. 2d).…”

Section: Visual-cc Can Induce Known Cc-related Gene Expressionsupporting

confidence: 76%

VISUAL-CC system uncovers the role of GSK3 as an orchestrator of vascular cell type ratio in plants

et al. 2020

View full text Add to dashboard Cite

The phloem transports photosynthetic assimilates and signalling molecules. It mainly consists of sieve elements (SEs), which act as "highways" for transport, and companion cells (CCs), which serve as "gates" to load/unload cargos. Though SEs and CCs function together, it remains unknown what determines the ratio of SE/CC in the phloem. Here we develop a new culture system for CC differentiation in Arabidopsis named VISUAL-CC, which almost mimics the process of the SE-CC complex formation. Comparative expression analysis in VISUAL-CC reveals that SE and CC differentiation tends to show negative correlation, while total phloem differentiation is unchanged. This varying SE/CC ratio is largely dependent on GSK3 kinase activity. Indeed, gsk3 hextuple mutants possess many more SEs and fewer CCs, whereas gsk3 gain-of-function mutants partially increase the CC number. Taken together, GSK3 activity appears to function as a cell-fate switch in the phloem, thereby balancing the SE/CC ratio.

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“…The low N-produced CEPs act as a long-distance signal through shoot-located XYLEM INTERMIXED WITH PHLOEM 1 (XIP1)/CEP RECEPTOR 1(CEPR1) to up-regulate high-affinity nitrate transporter genes in roots under local high N (Tabata et al, 2014). Further study reveals that auxin-repressive CEP5 inhibits LR initiation probably depending on XIP1/CEPR1 under local high N (Roberts et al, 2016). However, due to restrictions of the extract technique, adaptable methods and approaches on CEPs as well as a lack of a XIP1/CEPR1 knockout mutant, it is much more difficult to gain a complete understanding of CEP signal regulating LR development.…”

Section: Introductionmentioning

confidence: 91%

Nitrate: A Crucial Signal during Lateral Roots Development

2017

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Root plasticity is an important trait for plants to forage nutrient and adapt to survival in a complicated environment. Lateral roots (LRs) are generally more sensitive than primary roots in response to changing environmental conditions. As the main source of nitrogen for most higher plants, nitrate acting as a signal has received great attention in the regulation of LR development. In general, there are dual effects including stimulatory and inhibitory of low nitrate on LR development; while high nitrate supply has an inhibitory effect on LR development; nitrate heterogeneity also has a stimulatory effect on LR development in NO3–- rich zone. Here, we focus on recent progresses in the role of a nitrate signal in the regulation of the LRs development.

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CEP5 and XIP1/CEPR1 regulate lateral root initiation in Arabidopsis

Abstract: HighlightWe identified C-TERMINALLY ENCODED PEPTIDE 5 (CEP5) as a novel, auxin-repressed and phloem pole-expressed signal assisting in the formation of lateral roots.

Cited by 84 publications

References 50 publications

GSK3 activity is a cell fate switch that balances the ratio of vascular cell type

GSK3 activity is a cell fate switch that balances the ratio of vascular cell type

VISUAL-CC system uncovers the role of GSK3 as an orchestrator of vascular cell type ratio in plants

Nitrate: A Crucial Signal during Lateral Roots Development

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