Centrosome to autophagosome signaling: Specific GABARAP regulation by centriolar satellites

Joachim, Justin; Tooze, Sharon A.

doi:10.1080/15548627.2017.1385677

Cited by 8 publications

(6 citation statements)

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“…As prophase progresses to prometaphase, the nuclear envelope dismantles, microtubules are extended to the exposed chromatin and the centrosomes, the nucleation sites from where the tubulin filaments grow and travel to the opposing ends of the chromatin to form the mitotic spindle (Dey and Baum, 2021). Centrosomes serve as the main microtubuleorganizing centers of the cells and several autophagy-regulating proteins including microtubule-associated proteins 1 light chain 3B (LC3B), gamma-aminobutyric acid receptor-associated protein (GABARAP), sequestesome-1 and WD repeat domain phosphoinositide-interacting protein 2 (WIPI2) and have been detected at the centrosome region in mitotic cells (Joachim and Tooze, 2017;Holdgaard et al, 2019). Interestingly, a FIGURE 1 | Cell cycle-dependent switch of autophagic master regulator.…”

Section: Targeted Autophagy Stabilizes Centrosomesmentioning

confidence: 99%

They Might Cut It—Lysosomes and Autophagy in Mitotic Progression

Hämälistö

Stahl-Meyer

Jäättelä

2021

Front. Cell Dev. Biol.

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The division of one cell into two looks so easy, as if it happens without any control at all. Mitosis, the hallmark of mammalian life is, however, tightly regulated from the early onset to the very last phase. Despite the tight control, errors in mitotic division occur frequently and they may result in various chromosomal instabilities and malignancies. The flow of events during mitotic progression where the chromosomes condensate and rearrange with the help of the cytoskeletal network has been described in great detail. Plasma membrane dynamics and endocytic vesicle movement upon deadhesion and reattachment of dividing cells are also demonstrated to be functionally important for the mitotic integrity. Other cytoplasmic organelles, such as autophagosomes and lysosomes, have until recently been considered merely as passive bystanders in this process. Accordingly, at the onset of nuclear envelope breakdown in prometaphase, the number of autophagic structures and lysosomes is reduced and the bulk autophagic machinery is suppressed for the duration of mitosis. This is believed to ensure that the exposed nuclear components are not unintentionally delivered to autophagic degradation. With the evolving technologies that allow the detection of subtle alterations in cytoplasmic organelles, our understanding of the small-scale regulation of intracellular organelles has deepened rapidly and we discuss here recent discoveries revealing unexpected roles for autophagy and lysosomes in the preservation of genomic integrity during mitosis.

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Section: Targeted Autophagy Stabilizes Centrosomesmentioning

confidence: 99%

They Might Cut It—Lysosomes and Autophagy in Mitotic Progression

Hämälistö

Stahl-Meyer

Jäättelä

2021

Front. Cell Dev. Biol.

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“…In this context, it is tempting to speculate that CS are able to sequester compromised or superfluous centrosomal proteins that are subsequently targeted for autophagic destruction. However, additional work suggested that PCM1 retains GABARAP in an inactive state at CS, and it is also plausible that CS act to adjust autophagic flux by releasing the “dormant” CS-associated GABARAP pool to the centrosome [ 12 , 36 ].…”

Section: Introductionmentioning

confidence: 99%

Osmotic Stress Blocks Mobility and Dynamic Regulation of Centriolar Satellites

Nielsen

Nordgaard

Tollenaere

et al. 2018

Cells

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Centriolar satellites (CS) are small proteinaceous granules that cluster around the centrosome and serve as cargo vehicles for centrosomal proteins. It is generally accepted that CS support a number of canonical and specialized centrosome functions. Consequently, these highly dynamic structures are the target of regulation by several cellular signalling pathways. Two decades of research have led to the identification of a large number of molecular components and new biological roles of CS. Here, we summarize the latest advances in the continuous efforts to uncover the compositional, functional, dynamic and regulatory aspects of CS. We also report on our discovery that osmotic stress conditions render CS immobile and insensitive to remodelling. Upon a range of p38-activating stimuli, MK2 phosphorylates the CS component CEP131, resulting in 14-3-3 binding and a block to CS formation. This normally manifests as a rapid cellular depletion of satellites. In the case of osmotic stress, a potent inducer of p38 activity, CS translocation and dissolution is blocked, with the net result that satellites persist in an immobile state directly adjacent to the centrosome. Our results highlight a unique scenario where p38 activation and CS depletion is uncoupled, with potential implications for physiological and pathological osmotic stress responses.

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“…It has also been suggested that CS, at a distance from the centrosome, keep protein degradation machinery away from centrosome to prevent untimely ubiquitination and degradation of centrosomal components 14 . In fact, CS have been linked to ubiquitination, autophagy and the control of proteolysis in the cell [50][51][52][53][54][55][56] . Consequently, we believe that positioning CS closer to the centrosome in Kif9 depleted cells, in turn, positions the proteolytic machinery closer to centrosome-associated substrates leading to excess proteolysis.…”

Section: Kif9 Depletion Phenotypes In Mitosis Can Be Rescued By Inhib...mentioning

confidence: 99%

The kinesin motor Kif9 regulates centriolar satellite positioning and mitotic progression

Vicente,

Wagenbach,

Decarreau

et al. 2024

Preprint

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Centrosomes are the principal microtubule-organizing centers of the cell and play an essential role in mitotic spindle function. Centrosome biogenesis is achieved by strict control of protein acquisition and phosphorylation prior to mitosis. Defects in this process promote fragmentation of pericentriolar material culminating in multipolar spindles and chromosome missegregation. Centriolar satellites, membrane- less aggrupations of proteins involved in the trafficking of proteins toward and away from the centrosome, are thought to contribute to centrosome biogenesis. Here we show that the microtubule plus-end directed kinesin motor Kif9 localizes to centriolar satellites and regulates their pericentrosomal localization during interphase. Lack of Kif9 leads to aggregation of satellites closer to the centrosome and increased centrosomal protein degradation that disrupts centrosome maturation and results in chromosome congression and segregation defects during mitosis. Our data reveal roles for Kif9 and centriolar satellites in the regulation of cellular proteostasis and mitosis.

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Centrosome to autophagosome signaling: Specific GABARAP regulation by centriolar satellites

Cited by 8 publications

References 0 publications

They Might Cut It—Lysosomes and Autophagy in Mitotic Progression

They Might Cut It—Lysosomes and Autophagy in Mitotic Progression

Osmotic Stress Blocks Mobility and Dynamic Regulation of Centriolar Satellites

The kinesin motor Kif9 regulates centriolar satellite positioning and mitotic progression

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