Centrosomal protein 55: A new paradigm in tumorigenesis

Tandon, Divya; Banerjee, Monisha

doi:10.1016/j.ejcb.2020.151086

Cited by 12 publications

(10 citation statements)

References 67 publications

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“…As evident from recent findings, centrosomal protein ( CEP ) plays an important role in carcinogenesis. For example, CEP55 overexpression increases cancer cell stemness and enhances tumor formation by activating the PI3K/AKT pathway [ 31 ]. TACC3 , a key centrosomal protein, was upregulated in prostate cancer, and its silencing inhibited tumor growth [ 32 ].…”

Section: Discussionmentioning

confidence: 99%

Centrosomal protein 290 is a novel prognostic indicator that modulates liver cancer cell ferroptosis via the Nrf2 pathway

Shan

Yang

et al. 2022

Aging

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Ferroptosis is an iron-dependent form of cell death. In spite of its significance in pathogenesis and disease progression, ferroptotic signal transduction in HBV-HCC has not been fully explained. Here, four HCC opensource datasets were downloaded from the GEO repository. Cox regression and LASSO models were established to prioritize novel prognostic candidate biomarkers, and the results were verified in vitro and in vivo. We identified 633 common DEGs in both of the bulk RNA-Seq expression profiles. Next, based upon the TCGA-LIHC cohort, a prognostic signature consisting of nine genes was extracted from 633 shared DEGs, and the specificity and sensitivity of the signature were evaluated in both training and validation datasets. This signature showed that the high-risk group had a worse prognosis than the low-risk group. CEP290 was discovered among the prognostic signature genes, and its expression notably correlated with survival, AFP level, TNM stage and vascular invasion. We confirmed expression of CEP290 in eight pairs of HCC tissues and diverse liver cancer cell lines. CEP290 knockdown reduced proliferation, migration and invasion in Hep3B liver cancer cells while Fe2+ and malondialdehyde levels were elevated. Mechanically, co-immunoprecipitation showed an interaction between CEP290 and Nrf2 proteins, and biological phenotypes of Hep3B cells under CEP290 interference were rescued by Nrf2 activator. Furthermore, CEP290 silencing considerably blocked protein expression of Nrf2 pathway members. Finally, suppression of CEP290 effectively inhibited tumor growth in vivo. The above results shed light on the important role of CEP290 in ferroptosis and present an important implication for HCC progression.

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Section: Discussionmentioning

confidence: 99%

Centrosomal protein 290 is a novel prognostic indicator that modulates liver cancer cell ferroptosis via the Nrf2 pathway

Shan

Yang

et al. 2022

Aging

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“…Recently studies have demonstrated that CEP55 could promote cancer cell stemness and tumor formation through regulating the PI3K/AKT pathway. Clinically, Cep55 has also been found to be overexpressed in many cancer types, and its overexpression has been strikingly associated with tumor stage and metastasis ( Tandon and Banerjee, 2020 ). We demonstrated that, compared with Fn-non-infected Caco-2 cells, the relative expression of CEP55 was significantly higher in Fn-infected Caco-2 cells and knockdown of CEP55 inhibited cell proliferation and induced cell apoptosis in these cells.…”

Section: Discussionmentioning

confidence: 99%

Identification and Validation of Potential Candidate Genes of Colorectal Cancer in Response to Fusobacterium nucleatum Infection

Zhang

Wang

et al. 2021

Front. Genet.

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Objective: Recent investigations revealed the relationship between Fusobacterium nucleatum (Fn) infection and colorectal cancer (CRC). However, how the host genes changes contribute to CRC in response to Fn infection remains largely unknown.Materials and methods: In the present study, we aimed to comprehensively analyze microarray data obtained from a Caco-2 infection cell model using integrated bioinformatics analysis and further identify and validate potential candidate genes in Fn-infected Caco-2 cells and CRC specimens.Results: We identified 10 hub genes potentially involved in Fn induced tumor initiation and progression. Furthermore, we demonstrated that the expression of centrosomal protein of 55 kDa (CEP55) is significantly higher in Fn-infected Caco-2 cells. Knocking down of CEP55 could arrest the cell cycle progression and induce apoptosis in Fn-infected Caco-2 cells. The expression of CEP55 was positively correlated with the Fn amount in Fn-infected CRC patients, and these patients with high CEP55expression had an obviously poorer differentiation, worse metastasis and decreased cumulative survival rate.Conclusion: CEP55 plays an important role in Fn-infected colon cancer cell growth and cell cycle progression and could be used as a new diagnostic and prognostic biomarker for Fn-infected CRC.

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“…On the other hand, and not surprisingly, failures in the process of abscission, by leading to cells with an unstable tetraploid content, are, in general, connected with tumorigenesis [91]. Under this respect, different roles in cancer development are emerging for CEP55 [92]. Furthermore, it has been reported that when full chromosomes or part of them do not properly segregate, they can recruit their own nuclear membrane and form micronuclei (MN).…”

Section: Role Of the Escrt Machinery In Cytokinesismentioning

confidence: 99%

Why Cells and Viruses Cannot Survive without an ESCRT

Calistri

Reale

Palù

et al. 2021

Cells

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Intracellular organelles enwrapped in membranes along with a complex network of vesicles trafficking in, out and inside the cellular environment are one of the main features of eukaryotic cells. Given their central role in cell life, compartmentalization and mechanisms allowing their maintenance despite continuous crosstalk among different organelles have been deeply investigated over the past years. Here, we review the multiple functions exerted by the endosomal sorting complex required for transport (ESCRT) machinery in driving membrane remodeling and fission, as well as in repairing physiological and pathological membrane damages. In this way, ESCRT machinery enables different fundamental cellular processes, such as cell cytokinesis, biogenesis of organelles and vesicles, maintenance of nuclear–cytoplasmic compartmentalization, endolysosomal activity. Furthermore, we discuss some examples of how viruses, as obligate intracellular parasites, have evolved to hijack the ESCRT machinery or part of it to execute/optimize their replication cycle/infection. A special emphasis is given to the herpes simplex virus type 1 (HSV-1) interaction with the ESCRT proteins, considering the peculiarities of this interplay and the need for HSV-1 to cross both the nuclear-cytoplasmic and the cytoplasmic-extracellular environment compartmentalization to egress from infected cells.

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Centrosomal protein 55: A new paradigm in tumorigenesis

Cited by 12 publications

References 67 publications

Centrosomal protein 290 is a novel prognostic indicator that modulates liver cancer cell ferroptosis via the Nrf2 pathway

Centrosomal protein 290 is a novel prognostic indicator that modulates liver cancer cell ferroptosis via the Nrf2 pathway

Identification and Validation of Potential Candidate Genes of Colorectal Cancer in Response to Fusobacterium nucleatum Infection

Why Cells and Viruses Cannot Survive without an ESCRT

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